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TFE3 (NM_006521) Human Tagged ORF Clone

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SKU
RC206840
TFE3 (Myc-DDK-tagged)-Human transcription factor binding to IGHM enhancer 3 (TFE3)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $535.00
In Stock*
Size
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TFE3
Synonyms bHLHe33; RCCP2; RCCX1; TFEA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206840 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCATGCGGCCGAACCAGCTCGGGATGGCGTAGAGGCCAGCGCGGAGGGCCCTCGAGCCGTGTTCG
TGCTGTTGGAGGAGCGCAGGCCGGCCGACTCGGCTCAGCTGCTCAGCCTGAACTCTTTGCTTCCGGAATC
CGGGATTGTTGCTGACATAGAATTAGAAAACGTCCTTGATCCTGACAGCTTCTACGAGCTCAAAAGCCAA
CCCTTACCCCTTCGCTCAAGCCTCCCAATATCACTGCAGGCCACACCAGCCACCCCAGCTACACTCTCTG
CATCGTCTTCTGCAGGGGGCTCCAGGACCCCTGCCATGTCGTCATCTTCTTCATCGAGGGTCTTGCTGCG
GCAGCAGCTAATGCGGGCCCAGGCGCAGGAGCAGGAGAGGCGTGAGCGTCGGGAACAGGCCGCCGCGGCT
CCCTTCCCCAGTCCTGCACCTGCCTCTCCTGCCATCTCTGTGGTTGGCGTCTCTGCTGGGGGCCACACAT
TGAGCCGTCCACCCCCTGCTCAGGTGCCCAGGGAGGTGCTCAAGGTGCAGACCCATCTGGAGAACCCAAC
GCGCTACCACCTGCAGCAGGCGCGCCGGCAGCAGGTGAAACAGTACCTGTCCACCACACTCGGGCCCAAG
CTGGCTTCCCAGGCCCTCACCCCACCGCCGGGGCCCGCAAGTGCCCAGCCACTGCCTGCCCCTGAGGCTG
CCCACACTACCGGCCCCACAGGCAGTGCGCCCAACAGCCCCATGGCGCTGCTCACCATCGGGTCCAGCTC
AGAGAAGGAGATTGATGATGTCATTGATGAGATCATCAGCCTGGAGTCCAGTTACAATGATGAAATGCTC
AGCTATCTGCCCGGAGGCACCACAGGACTGCAGCTCCCCAGCACGCTGCCTGTGTCAGGGAATCTGCTTG
ATGTGTACAGTAGTCAAGGCGTGGCCACACCAGCCATCACTGTCAGCAACTCCTGCCCAGCTGAGCTGCC
CAACATCAAACGGGAGATCTCTGAGACCGAGGCAAAGGCCCTTTTGAAGGAACGGCAGAAGAAAGACAAT
CACAACCTAATTGAGCGTCGCAGGCGATTCAACATTAACGACAGGATCAAGGAACTGGGCACTCTCATCC
CTAAGTCCAGTGACCCGGAGATGCGCTGGAACAAGGGCACCATCCTGAAGGCCTCTGTGGATTATATCCG
CAAGCTGCAGAAGGAGCAGCAGCGCTCCAAAGACCTGGAGAGCCGGCAGCGATCCCTGGAGCAGGCCAAC
CGCAGCCTGCAGCTCCGAATTCAGGAACTAGAACTGCAGGCCCAGATCCATGGCCTGCCAGTGCCTCCCA
CTCCAGGGCTGCTTTCCTTGGCCACGACTTCGGCTTCTGACAGCCTCAAGCCAGAGCAGCTGGACATTGA
GGAGGAGGGCAGGCCAGGCGCAGCAACGTTCCATGTAGGGGGGGGACCTGCCCAGAATGCTCCCCATCAG
CAGCCCCCTGCACCGCCCTCAGATGCCCTTCTGGACCTGCACTTTCCCAGCGACCACCTGGGGGACCTGG
GAGACCCCTTCCACCTGGGGCTGGAGGACATTCTGATGGAGGAGGAGGAGGGGGTGGTGGGAGGACTGTC
GGGGGGTGCCCTGTCCCCACTGCGGGCTGCCTCCGATCCCCTGCTCTCTTCAGTGTCCCCTGCTGTCTCC
AAGGCCAGCAGCCGCCGCAGCAGCTTCAGCATGGAAGAGGAGTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206840 protein sequence
Red=Cloning site Green=Tags(s)

MSHAAEPARDGVEASAEGPRAVFVLLEERRPADSAQLLSLNSLLPESGIVADIELENVLDPDSFYELKSQ
PLPLRSSLPISLQATPATPATLSASSSAGGSRTPAMSSSSSSRVLLRQQLMRAQAQEQERRERREQAAAA
PFPSPAPASPAISVVGVSAGGHTLSRPPPAQVPREVLKVQTHLENPTRYHLQQARRQQVKQYLSTTLGPK
LASQALTPPPGPASAQPLPAPEAAHTTGPTGSAPNSPMALLTIGSSSEKEIDDVIDEIISLESSYNDEML
SYLPGGTTGLQLPSTLPVSGNLLDVYSSQGVATPAITVSNSCPAELPNIKREISETEAKALLKERQKKDN
HNLIERRRRFNINDRIKELGTLIPKSSDPEMRWNKGTILKASVDYIRKLQKEQQRSKDLESRQRSLEQAN
RSLQLRIQELELQAQIHGLPVPPTPGLLSLATTSASDSLKPEQLDIEEEGRPGAATFHVGGGPAQNAPHQ
QPPAPPSDALLDLHFPSDHLGDLGDPFHLGLEDILMEEEEGVVGGLSGGALSPLRAASDPLLSSVSPAVS
KASSRRSSFSMEEES

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006521
ORF Size 1725 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006521.3, NP_006512.2
RefSeq Size 3467 bp
RefSeq ORF 1728 bp
Locus ID 7030
UniProt ID P19532
Cytogenetics Xp11.23
Domains HLH
Protein Families Druggable Genome, Transcription Factors
MW 61.5 kDa
Summary This gene encodes a basic helix-loop-helix domain-containing transcription factor that binds MUE3-type E-box sequences in the promoter of genes. The encoded protein promotes the expression of genes downstream of transforming growth factor beta (TGF-beta) signaling. This gene may be involved in chromosomal translocations in renal cell carcinomas and other cancers, resulting in the production of fusion proteins. Translocation partners include PRCC (papillary renal cell carcinoma), NONO (non-POU domain containing, octamer-binding), and ASPSCR1 (alveolar soft part sarcoma chromosome region, candidate 1), among other genes. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2013
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Documents
Resources
"NM_006521" in other vectors (7)
SKU Description Size Price
RC206840L1 Lenti ORF clone of Human transcription factor binding to IGHM enhancer 3 (TFE3), Myc-DDK-tagged 10 ug
$835.00
RC206840L2 Lenti ORF clone of Human transcription factor binding to IGHM enhancer 3 (TFE3), mGFP tagged 10 ug
$835.00
RC206840L3 Lenti ORF clone of Human transcription factor binding to IGHM enhancer 3 (TFE3), Myc-DDK-tagged 10 ug
$835.00
RC206840L4 Lenti ORF clone of Human transcription factor binding to IGHM enhancer 3 (TFE3), mGFP tagged 10 ug
$835.00
RG206840 TFE3 (tGFP-tagged) - Human transcription factor binding to IGHM enhancer 3 (TFE3) 10 ug
$489.00 $735.00
SC116014 TFE3 (untagged)-Human transcription factor binding to IGHM enhancer 3 (TFE3) 10 ug
$536.00
SC320562 TFE3 (untagged)-Human transcription factor binding to IGHM enhancer 3 (TFE3) 10 ug
$536.00

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