DEDD2 (NM_133328) Human Tagged ORF Clone

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SKU
RC206798
DEDD2 (Myc-DDK-tagged)-Human death effector domain containing 2 (DEDD2)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DEDD2
Synonyms FLAME-3; FLAME3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206798 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCTATCCGGGTCGACCCCGGCCCCGTGCTGGGAGGAGGATGAGTGCCTGGACTACTACGGGATGC
TGTCGCTTCACCGTATGTTCGAGGTGGTGGGCGGGCAACTGACCGAGTGCGAGCTGGAGCTCCTGGCCTT
TCTGCTGGATGAGGCTCCTGGCGCCGCCGGAGGCTTAGCCCGGGCCCGCAGCGGCCTAGAGCTCCTGCTG
GAGCTGGAGCGCCGCGGGCAGTGCGACGAGAGCAACCTGCGGCTGCTGGGGCAACTCCTGCGCGTGCTGG
CCCGCCACGACCTGCTGCCGCACCTGGCGCGCAAGCGGCGCCGGCCAGTGTCTCCAGAACGCTATAGCTA
TGGCACCTCCAGCTCTTCAAAGAGGACAGAGGGTAGCTGCCGTCGCCGTCGGCAGTCAAGCAGTTCTGCA
AATTCTCAGCAGGGTCAGTGGGAGACAGGCTCCCCCCCAACCAAGCGGCAGCGGCGGAGTCGGGGCCGGC
CCAGTGGTGGTGCCAGACGGCGGCGGAGAGGGGCCCCAGCCGCACCCCAGCAGCAGTCAGAGCCCGCCAG
ACCTTCCTCTGAAGGCAAAGTGACCTGTGACATCCGGCTCCGGGTTCGAGCAGAGTACTGCGAGCATGGG
CCAGCCTTGGAGCAGGGCGTGGCATCCCGGCGGCCCCAGGCGCTGGCGCGGCAGCTGGACGTGTTTGGGC
AGGCCACCGCAGTGCTGCGCTCAAGGGACCTGGGCTCCGTGGTTTGTGACATCAAGTTCTCAGAGCTCTC
CTATCTGGACGCCTTCTGGGGCGACTACCTGAGTGGTGCCCTGCTGCAGGCCCTGCGGGGCGTGTTCCTG
ACTGAGGCCCTGCGAGAGGCTGTGGGCCGGGAGGCTGTTCGCCTGCTGGTCAGTGTGGATGAGGCTGACT
ATGAGGCTGGCCGGCGCCGCCTGTTGCTGATGGAGGAGGAAGGGGGGCGGCGCCCGACAGAGGCCTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC206798 protein sequence
Red=Cloning site Green=Tags(s)

MALSGSTPAPCWEEDECLDYYGMLSLHRMFEVVGGQLTECELELLAFLLDEAPGAAGGLARARSGLELLL
ELERRGQCDESNLRLLGQLLRVLARHDLLPHLARKRRRPVSPERYSYGTSSSSKRTEGSCRRRRQSSSSA
NSQQGQWETGSPPTKRQRRSRGRPSGGARRRRRGAPAAPQQQSEPARPSSEGKVTCDIRLRVRAEYCEHG
PALEQGVASRRPQALARQLDVFGQATAVLRSRDLGSVVCDIKFSELSYLDAFWGDYLSGALLQALRGVFL
TEALREAVGREAVRLLVSVDEADYEAGRRRLLLMEEEGGRRPTEAS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133328
ORF Size 978 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133328.2, NP_579874.1
RefSeq Size 2025 bp
RefSeq ORF 981 bp
Locus ID 162989
UniProt ID Q8WXF8
Cytogenetics 19q13.2
Domains DED
Protein Families Druggable Genome, Transcription Factors
MW 36.2 kDa
Summary This gene encodes a nuclear-localized protein containing a death effector domain (DED). The encoded protein may regulate the trafficking of caspases and other proteins into the nucleus during death receptor-induced apoptosis. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2012
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Documents
Resources
"NM_133328" in other vectors (7)
SKU Description Size Price
RC206798L1 Lenti ORF clone of Human death effector domain containing 2 (DEDD2), Myc-DDK-tagged 10 ug
$600.00
RC206798L2 Lenti ORF clone of Human death effector domain containing 2 (DEDD2), mGFP tagged 10 ug
$600.00
RC206798L3 Lenti ORF clone of Human death effector domain containing 2 (DEDD2), Myc-DDK-tagged 10 ug
$600.00
RC206798L4 Lenti ORF clone of Human death effector domain containing 2 (DEDD2), mGFP tagged 10 ug
$600.00
RG206798 DEDD2 (tGFP-tagged) - Human death effector domain containing 2 (DEDD2) 10 ug
$500.00
SC108318 DEDD2 (untagged)-Human death effector domain containing 2 (DEDD2) 10 ug
$300.00
SC323825 DEDD2 (untagged)-Human death effector domain containing 2 (DEDD2) 10 ug
$300.00

Plasmid Map for RC206798

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Cloning scheme for RC206798

Circular map for RC206798

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