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DUOXA1 (NM_144565) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC206754
DUOXA1 (Myc-DDK-tagged)-Human dual oxidase maturation factor 1 (DUOXA1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $686.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DUOXA1
Synonyms mol; NIP; NUMBIP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206754 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTACTTTGGGACACACATTCCCCTTCTATGCTGGCCCCAAGCCAACCTTCCCGATGGACACCACTT
TGGCCAGCATCATCATGATCTTTCTGACTGCACTGGCCACGTTCATCGTCATCCTGCCTGGCATTCGGGG
AAAGACGAGGCTGTTCTGGCTGCTTCGGGTGGTGACCAGCTTATTCATCGGGGCTGCAATCCTGGCTGTG
AATTTCAGTTCTGAGTGGTCTGTGGGCCAGGTCAGCACCAACACATCATACAAGGCCTTCAGTTCTGAGT
GGATCAGCGCTGATATTGGGCTGCAGGTCGGGCTGGGTGGAGTCAACATCACACTCACAGGGACCCCCGT
GCAGCAGCTGAATGAGACCATCAATTACAACGAGGAGTTCACCTGGCGCCTGGGTGAGAACTATGCTGAG
GAGTATGCAAAGGCTCTGGAGAAGGGGCTGCCAGACCCTGTGTTGTACCTAGCTGAGAAGTTCACTCCAA
GAAGCCCATGTGGCCTATACCGCCAGTACCGCCTGGCGGGACACTACACCTCAGCCATGCTATGGGTGGC
ATTCCTCTGCTGGCTGCTGGCCAATGTGATGCTCTCCATGCCTGTGCTGGTATATGGTGGCTACATGCTA
TTGGCCACGGGCATCTTCCAGCTGTTGGCTCTGCTCTTCTTCTCCATGGCCACATCACTCACCTCACCCT
GTCCCCTGCACCTGGGCGCTTCTGTGCTGCATACTCACCATGGGCCTGCCTTCTGGATCACATTGACCAC
AGGACTGCTGTGTGTGCTGCTGGGCCTGGCTATGGCGGTGGCCCACAGGATGCAGCCTCACAGGCTGAAG
GCTTTCTTCAACCAGAGTGTGGATGAAGACCCCATGCTGGAGTGGAGTCCTGAGGAAGGTGGACTCCTGA
GCCCCCGCTACCGGTCCATGGCTGACAGTCCCAAGTCCCAGGACATTCCCCTGTCAGAGGCTTCCTCCAC
CAAGGCATACTATCGCCCCAGGAGACTTTCCCTGGTGCCTGCGGATGTCCGAGGCCTCGCGCCAGCAGCG
CTCAGTGCCCTTCCTGGAGCTCTCCTGGCCCAGGCCTGGCGGGCACTGCTTCCCGGCCTGCGATGTCCCA
AGGCGGGGAAGGAGTCCAGATTGGGTCCCCCTCACAGTCCTTGGCGCTTTGGTCCAGAAGGGTGCGAAGA
GCGCTGGGCCGAACATACTGGAGACTCACCACGGCCCCTCCGAGGAAGAGGCACAGGACGCCTGTGGCGG
TGGGGATCGAAAGAAAGGAGGGCATGTGGAGTCAGGGCTATGTTGCCCAGGCTGGTCTCGAACTCTGGCC
TCAAACGACCTTCCTGCCTCGACCTCCCAAAGTGCTGGGATTACAGGCGTGATGCCCGGGCCTTCTTCCA
TCTTTTGGAGCCTACCCCTTGTGTTACCTCCCGCCACACACCTCTAATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206754 protein sequence
Red=Cloning site Green=Tags(s)

MATLGHTFPFYAGPKPTFPMDTTLASIIMIFLTALATFIVILPGIRGKTRLFWLLRVVTSLFIGAAILAV
NFSSEWSVGQVSTNTSYKAFSSEWISADIGLQVGLGGVNITLTGTPVQQLNETINYNEEFTWRLGENYAE
EYAKALEKGLPDPVLYLAEKFTPRSPCGLYRQYRLAGHYTSAMLWVAFLCWLLANVMLSMPVLVYGGYML
LATGIFQLLALLFFSMATSLTSPCPLHLGASVLHTHHGPAFWITLTTGLLCVLLGLAMAVAHRMQPHRLK
AFFNQSVDEDPMLEWSPEEGGLLSPRYRSMADSPKSQDIPLSEASSTKAYYRPRRLSLVPADVRGLAPAA
LSALPGALLAQAWRALLPGLRCPKAGKESRLGPPHSPWRFGPEGCEERWAEHTGDSPRPLRGRGTGRLWR
WGSKERRACGVRAMLPRLVSNSGLKRPSCLDLPKCWDYRRDARAFFHLLEPTPCVTSRHTPLI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144565
ORF Size 1449 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144565.4
RefSeq Size 2020 bp
RefSeq ORF 1452 bp
Locus ID 90527
UniProt ID Q1HG43, A8K9Q6
Cytogenetics 15q21.1
Protein Families Transmembrane
MW 53.5 kDa
Summary Dual oxidases DUOX1 and DUOX2 are NADPH oxidases which are involved in hydrogen peroxide production necessary for thyroid hormonogenesis. They form a heterodimer with specific maturation factors DUOXA1 and DUOXA2, respectively, which is essential for the maturation and function of the DUOX enzyme complexes. This gene encodes the DUOX1 activator or maturation factor DUOXA1. Rat studies identified a bidirectional promoter which controls the transcription of the DUOX1 and DUOXA1 genes. This protein is cotransported to the cell surface when coexpressed with DUOX1 and is retained in the endoplasmic reticulum when expressed without DUOX1 protein. The expression of this gene or the DUOX1 gene is not suppressed by thyroglobulin (Tg), a macromolecular precursor in thyroid hormone synthesis, while the expression of the DUOX2 and DUOXA2 are significantly suppressed by the Tg. This protein is also a p53-regulated neurogenic factor involved in p53 dependent neuronal differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jan 2013
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Documents
Resources
"NM_144565" in other vectors (4)
SKU Description Size Price
RC206754L3 Lenti ORF clone of Human dual oxidase maturation factor 1 (DUOXA1), Myc-DDK-tagged 10 ug
$986.00
RC206754L4 Lenti ORF clone of Human dual oxidase maturation factor 1 (DUOXA1), mGFP tagged 10 ug
$986.00
RG206754 DUOXA1 (tGFP-tagged) - Human dual oxidase maturation factor 1 (DUOXA1) 10 ug
$886.00
SC123168 DUOXA1 (untagged)-Human dual oxidase maturation factor 1 (DUOXA1) 10 ug
$686.00

Plasmid Map for RC206754

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206754
Circular map for RC206754

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