RTL6 (NM_032287) Human Tagged ORF Clone

SKU
RC206668
LDOC1L (Myc-DDK-tagged)-Human leucine zipper, down-regulated in cancer 1-like (LDOC1L)
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RTL6
Synonyms dJ1033E15.2; LDOC1L; Mar6; Mart6; SIRH3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206668 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCAGCCGCAGACGTCCAAAGCTGAAAGCCCAGCCTTGGCAGCGTCTCCGAATGCCCAGATGGATG
ACGTTATTGACACCCTGACCTCCCTGCGCCTCACCAACTCGGCGCTGAGGCGGGAGGCTTCCACCCTGCG
GGCGGAGAAGGCCAATCTCACCAACATGCTGGAGAGCGTGATGGCAGAGCTGACCTTGTTACGCACCAGG
GCGCGGATCCCGGGGGCTCTGCAGATCACCCCGCCCATCTCCTCAATTACTTCAAACGGGACTCGACCCA
TGACCACACCTCCAACCTCTCTGCCCGAGCCCTTTTCCGGGGACCCAGGCCGGTTGGCGGGGTTCCTGAT
GCAGATGGACAGATTCATGATCTTCCAGGCCTCCCGCTTCCCGGGTGAGGCCGAGCGTGTGGCCTTCCTT
GTGTCTCGACTGACTGGGGAGGCGGAGAAGTGGGCTATCCCCCACATGCAACCTGACAGCCCCTTGCGCA
ACAACTATCAGGGGTTCCTGGCAGAGTTGCGGAGAACCTACAAGTCTCCGCTCCGGCATGCGCGGCGCGC
CCAAATCAGGAAGACTTCTGCCTCTAATAGGGCTGTGCGAGAGAGGCAGATGCTCTGCCGCCAGCTGGCC
TCTGCGGGCACGGGGCCTTGCCCAGTGCATCCAGCTTCCAACGGGACTAGTCCAGCGCCAGCCCTGCCTG
CCCGAGCACGGAATCTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC206668 protein sequence
Red=Cloning site Green=Tags(s)

MVQPQTSKAESPALAASPNAQMDDVIDTLTSLRLTNSALRREASTLRAEKANLTNMLESVMAELTLLRTR
ARIPGALQITPPISSITSNGTRPMTTPPTSLPEPFSGDPGRLAGFLMQMDRFMIFQASRFPGEAERVAFL
VSRLTGEAEKWAIPHMQPDSPLRNNYQGFLAELRRTYKSPLRHARRAQIRKTSASNRAVRERQMLCRQLA
SAGTGPCPVHPASNGTSPAPALPARARNL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_032287
ORF Size 717 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032287.3
RefSeq Size 5355 bp
RefSeq ORF 720 bp
Locus ID 84247
UniProt ID Q6ICC9
Cytogenetics 22q13.31
MW 26.2 kDa
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Citations

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