CD72 (NM_001782) Human Tagged ORF Clone

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SKU
RC206609
CD72 (Myc-DDK-tagged)-Human CD72 molecule (CD72)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CD72
Synonyms CD72b; LYB2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206609 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGAGGCCATCACCTATGCAGATCTGAGGTTTGTGAAGGCTCCCCTGAAGAAGAGCATCTCCAGCC
GGTTAGGACAGGACCCAGGGGCTGATGATGATGGGGAAATCACCTACGAGAATGTTCAAGTGCCCGCAGT
CCTAGGGGTGCCCTCAAGCTTGGCTTCTTCTGTACTAGGGGACAAAGCAGCGGTCAAGTCGGAGCAGCCA
ACTGCGTCCTGGAGAGCCGTGACGTCACCAGCTGTCGGGCGGATTCTCCCCTGCCGCACAACCTGCCTGC
GATACCTCCTGCTCGGCCTGCTCCTCACCTGCCTGCTGTTAGGAGTGACCGCCATCTGCCTGGGAGTGCG
CTATCTGCAGGTGTCTCAGCAGCTCCAGCAGACGAACAGGGTTCTGGAAGTCACTAACAGCAGCCTGAGG
CAGCAGCTCCGCCTCAAGATAACGCAGCTGGGACAGAGTGCAGAGGATCTGCAGGGGTCCAGGAGAGAGC
TGGCGCAGAGTCAGGAAGCACTACAGGTGGAACAGAGGGCTCATCAGGCGGCCGAAGGGCAGCTACAGGC
CTGCCAGGCAGACAGACAGAAGACGAAGGAGACCTTGCAAAGTGAGGAGCAACAGAGGAGGGCCTTGGAG
CAGAAGCTGAGCAACATGGAGAACAGACTGAAGCCCTTCTTCACATGCGGCTCAGCAGACACCTGCTGTC
CGTCGGGATGGATAATGCATCAGAAAAGCTGCTTTTACATCTCACTTACTTCAAAAAATTGGCAGGAGAG
CCAAAAACAATGTGAAACTCTGTCTTCCAAGCTGGCCACATTCAGTGAAATTTATCCACAATCACACTCT
TACTACTTCTTAAATTCACTGTTGCCAAATGGTGGTTCAGGGAATTCATATTGGACTGGCCTCAGCTCTA
ACAAGGATTGGAAGTTGACTGATGATACACAACGCACTAGGACTTATGCTCAAAGCTCAAAATGTAACAA
GGTACATAAAACTTGGTCATGGTGGACACTGGAGTCAGAGTCATGTAGAAGTTCTCTTCCCTACATCTGT
GAGATGACAGCTTTCAGGTTTCCAGAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC206609 protein sequence
Red=Cloning site Green=Tags(s)

MAEAITYADLRFVKAPLKKSISSRLGQDPGADDDGEITYENVQVPAVLGVPSSLASSVLGDKAAVKSEQP
TASWRAVTSPAVGRILPCRTTCLRYLLLGLLLTCLLLGVTAICLGVRYLQVSQQLQQTNRVLEVTNSSLR
QQLRLKITQLGQSAEDLQGSRRELAQSQEALQVEQRAHQAAEGQLQACQADRQKTKETLQSEEQQRRALE
QKLSNMENRLKPFFTCGSADTCCPSGWIMHQKSCFYISLTSKNWQESQKQCETLSSKLATFSEIYPQSHS
YYFLNSLLPNGGSGNSYWTGLSSNKDWKLTDDTQRTRTYAQSSKCNKVHKTWSWWTLESESCRSSLPYIC
EMTAFRFPD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001782
ORF Size 1077 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001782.3
RefSeq Size 1548 bp
RefSeq ORF 1080 bp
Locus ID 971
UniProt ID P21854, Q5TLG3
Cytogenetics 9p13.3
Domains CLECT
Protein Families Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways B cell receptor signaling pathway
MW 40.2 kDa
Summary Plays a role in B-cell proliferation and differentiation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001782" in other vectors (6)
SKU Description Size Price
RC206609L1 Lenti ORF clone of Human CD72 molecule (CD72), Myc-DDK-tagged 10 ug
$757.00
RC206609L2 Lenti ORF clone of Human CD72 molecule (CD72), mGFP tagged 10 ug
$757.00
RC206609L3 Lenti ORF clone of Human CD72 molecule (CD72), Myc-DDK-tagged 10 ug
$757.00
RC206609L4 Lenti ORF clone of Human CD72 molecule (CD72), mGFP tagged 10 ug
$757.00
RG206609 CD72 (tGFP-tagged) - Human CD72 molecule (CD72) 10 ug
$489.00 $657.00
SC321779 CD72 (untagged)-Human CD72 molecule (CD72) 10 ug
$457.00

Plasmid Map for RC206609

Download sequence for

Cloning scheme for RC206609

Circular map for RC206609

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