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P2X6 (P2RX6) (NM_005446) Human Tagged ORF Clone

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SKU
RC206536
P2RX6 (Myc-DDK-tagged)-Human purinergic receptor P2X, ligand-gated ion channel, 6 (P2RX6), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol P2X6
Synonyms P2RXL1; P2X6; P2XM
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206536 representing NM_005446
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGCCCGCAGCTAGCAGGAGCTGGCAGCATGGGCTCCCCAGGGGCTACGACAGGCTGGGGGCTTCTGG
ATTATAAGACGGAGAAGTATGTGATGACCAGGAACTGGCGGGTGGGCGCCCTGCAGAGGCTGCTGCAGTT
TGGGATCGTGGTCTATGTGGTAGGGTGGGCTCTCCTCGCCAAAAAAGGCTACCAGGAGCGGGACCTGGAA
CCCCAGTTTTCCATCATCACCAAACTCAAAGGGGTTTCCGTCACTCAGATCAAGGAGCTTGGAAACCGGC
TGTGGGATGTGGCCGACTTCGTGAAGCCACCTCAGGGAGAGAACGTGTTCTTCTTGGTGACCAACTTCCT
TGTGACGCCAGCCCAAGTTCAGGGCAGATGCCCAGAGCACCCGTCCGTCCCACTGGCTAACTGCTGGGTC
GACGAGGACTGCCCCGAAGGGGAGGGAGGCACACACAGCCACGGTGTAAAAACAGGCCAGTGTGTGGTGT
TCAATGGGACCCACAGGACCTGTGAGATCTGGAGTTGGTGCCCAGTGGAGAGTGGCGTTGTGCCCTCGAG
GCCCCTGCTGGCCCAGGCCCAGAACTTCACACTGTTCATCAAAAACACAGTCACCTTCAGCAAGTTCAAC
TTCTCTAAGTCCAATGCCTTGGAGACCTGGGACCCCACCTATTTTAAGCACTGCCGCTATGAACCACAAT
TCAGCCCCTACTGTCCCGTGTTCCGCATTGGGGACCTCGTGGCCAAGGCTGGAGGGACCTTCGAGGACCT
GGCGTTGCTGGGTGGCTCTGTAGGCATCAGAGTTCACTGGGATTGTGACCTGGACACCGGGGACTCTGGC
TGCTGGCCTCACTACTCCTTCCAGCTGCAGGAGAAGAGCTACAACTTCAGGACAGCCACTCACTGGTGGG
AGCAACCGGGTGTGGAGGCCCGCACCCTGCTCAAGCTCTATGGAATCCGCTTCGACATCCTCGTCACCGG
GCAGGCAGGGAAGTTCGGGCTCATCCCCACGGCCGTCACACTGGGCACCGGGGCAGCTTGGCTGGGCGTG
GTCACCTTTTTCTGTGACCTGCTACTGCTGTATGTGGATAGAGAAGCCCATTTCTACTGGAGGACAAAGT
ATGAGGAGGCCAAGGCCCCGAAAGCAACCGCCAACTCTGTGTGGAGGGAGCTGGCCCTTGCATCCCAAGC
CCGACTGGCCGAGTGCCTCAGACGGAGCTCAGCACCTGCACCCACGGCCACTGCTGCTGGGAGTCAGACA
CAGACACCAGGATGGCCCTGTCCAAGTTCTGACACCCACTTGCCAACCCATTCCGGGAGCCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206536 representing NM_005446
Red=Cloning site Green=Tags(s)

MCPQLAGAGSMGSPGATTGWGLLDYKTEKYVMTRNWRVGALQRLLQFGIVVYVVGWALLAKKGYQERDLE
PQFSIITKLKGVSVTQIKELGNRLWDVADFVKPPQGENVFFLVTNFLVTPAQVQGRCPEHPSVPLANCWV
DEDCPEGEGGTHSHGVKTGQCVVFNGTHRTCEIWSWCPVESGVVPSRPLLAQAQNFTLFIKNTVTFSKFN
FSKSNALETWDPTYFKHCRYEPQFSPYCPVFRIGDLVAKAGGTFEDLALLGGSVGIRVHWDCDLDTGDSG
CWPHYSFQLQEKSYNFRTATHWWEQPGVEARTLLKLYGIRFDILVTGQAGKFGLIPTAVTLGTGAAWLGV
VTFFCDLLLLYVDREAHFYWRTKYEEAKAPKATANSVWRELALASQARLAECLRRSSAPAPTATAAGSQT
QTPGWPCPSSDTHLPTHSGSL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005446
ORF Size 1323 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005446.4
RefSeq Size 2754 bp
RefSeq ORF 1326 bp
Locus ID 9127
UniProt ID O15547
Cytogenetics 22q11.21
Protein Families Druggable Genome, Ion Channels: ATP Receptors, Transmembrane
Protein Pathways Calcium signaling pathway, Neuroactive ligand-receptor interaction
MW 48.6 kDa
Summary The protein encoded by this gene belongs to the family of P2X receptors, which are ATP-gated ion channels and mediate rapid and selective permeability to cations. This gene is predominantly expressed in skeletal muscle, and regulated by p53. The encoded protein is associated with VE-cadherin at the adherens junctions of human umbilical vein endothelial cells. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 22, has been identified. provided by RefSeq, Apr 2009
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"NM_005446" in other vectors (5)
SKU Description Size Price
RC206536L3 Lenti ORF clone of Human purinergic receptor P2X, ligand-gated ion channel, 6 (P2RX6), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC206536L4 Lenti ORF clone of Human purinergic receptor P2X, ligand-gated ion channel, 6 (P2RX6), transcript variant 1, mGFP tagged 10 ug
$757.00
RG206536 P2RX6 (tGFP-tagged) - Human purinergic receptor P2X, ligand-gated ion channel, 6 (P2RX6), transcript variant 1 10 ug
$489.00 $657.00
SC317723 P2RX6 (untagged)-Human purinergic receptor P2X, ligand-gated ion channel, 6 (P2RX6), transcript variant 1 10 ug
$457.00
SC317724 P2RX6 (untagged)-Human purinergic receptor P2X, ligand-gated ion channel, 6 (P2RX6), transcript variant 1 10 ug
$503.00

Plasmid Map for RC206536

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206536
Circular map for RC206536

file-alt Citations

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