MX2 (NM_002463) Human Tagged ORF Clone
$982.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | MX2 |
| Synonyms | MXB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002463 |
| ORF Size | 2145 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002463.2 |
| RefSeq Size | 2961 bp |
| RefSeq ORF | 2148 bp |
| Locus ID | 4600 |
| UniProt ID | P20592 |
| Cytogenetics | 21q22.3 |
| Domains | dynamin, dynamin_2, GED |
| Protein Families | Druggable Genome |
| MW | 82.1 kDa |
| Summary | The protein encoded by this gene has a nuclear and a cytoplasmic form and is a member of both the dynamin family and the family of large GTPases. The nuclear form is localized in a granular pattern in the heterochromatin region beneath the nuclear envelope. A nuclear localization signal (NLS) is present at the amino terminal end of the nuclear form but is lacking in the cytoplasmic form due to use of an alternate translation start codon. This protein is upregulated by interferon-alpha but does not contain the antiviral activity of a similar myxovirus resistance protein 1. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002463" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC206437L1 | Lenti ORF clone of Human myxovirus (influenza virus) resistance 2 (mouse) (MX2), Myc-DDK-tagged | 10 ug |
$1,282.00
|
|
| RC206437L2 | Lenti ORF clone of Human myxovirus (influenza virus) resistance 2 (mouse) (MX2), mGFP tagged | 10 ug |
$1,282.00
|
|
| RC206437L3 | Lenti ORF clone of Human myxovirus (influenza virus) resistance 2 (mouse) (MX2), Myc-DDK-tagged | 10 ug |
$1,282.00
|
|
| RC206437L4 | Lenti ORF clone of Human myxovirus (influenza virus) resistance 2 (mouse) (MX2), mGFP tagged | 10 ug |
$1,282.00
|
|
| RG206437 | MX2 (tGFP-tagged) - Human myxovirus (influenza virus) resistance 2 (mouse) (MX2) | 10 ug |
$1,182.00
|
|
| SC127459 | MX2 (untagged)-Human myxovirus (influenza virus) resistance 2 (mouse) (MX2) | 10 ug |
$983.00
|
|
Citations
Massively parallel reporter assays of melanoma risk variants identify MX2 as a gene promoting melanoma
Choi, Zhang, Vu et al
Nat Commun (2020) 11 (1), 2718 DOI: 10.1038/s41467-020-16590-1
Choi, Zhang, Vu et al
Nat Commun (2020) 11 (1), 2718 DOI: 10.1038/s41467-020-16590-1
Cited by 144
AI Summary: OriGene’s MX2 cDNA clone (RC206437, pCMV6-entry backbone) was used as the source of full-length MX2 sequence for functional studies in this paper, enabling the authors to investigate the role of MX2 in melanoma susceptibility.
Snippet: entiviral transduction of MX2 cDNA under the control of tetracycline-inducible promoter using pINDUCER20 vector (Addgene). The MX2 cDNA clone (RC206437) in the pCMV6-entry backbone was purchased from Origene and full-length MX2 cDNA sequence was sub-cloned to pENTR-1A vector by introducing stop codons and removing 3’ Myc-DDK tag before being transferred to pINDUCER20 vector (adapter sequence is
Naturally Occurring Mutations in HIV-1 CRF01_AE Capsid Affect Viral Sensitivity to Restriction Factors
Nakayama, Saito, Sultana et al
AIDS Res. Hum. Retroviruses (2018) 34 (4), 382-392 DOI: 10.1089/AID.2017.0212
Nakayama, Saito, Sultana et al
AIDS Res. Hum. Retroviruses (2018) 34 (4), 382-392 DOI: 10.1089/AID.2017.0212
AI Summary: OriGene’s human MxB cDNA was used as a template to generate recombinant Sendai virus vectors expressing HA-tagged MxB, enabling the study of how HIV-1 capsid mutations affect viral sensitivity to the MxB restriction factor.
Snippet: Sendai viruses (SeV) encoding human TRIM5α or cynomolgus monkey (CM) TRIM5α without the SPRY domain [CM-SPRY (−)] were described previously.21,27,28 Sequences encoding human MxB were purchased from Origene and used as a template to generate a recombinant SeV vector expressing MxB with an HA-tag in its C-terminus
Massively parallel reporter assays combined with cell-type specific eQTL informed multiple melanoma loci and identified a pleiotropic function of HIV-1 restriction gene, MX2, in melanoma promotion
Choi, Zhang, Vu et al
BioRxiv (2019)
Choi, Zhang, Vu et al
BioRxiv (2019)
Snippet: The MX2 cDNA clone (RC206437) in the pCMV6-entry backbone was purchased from Origene and full-length MX2 cDNA sequence was sub-cloned to pENTR-1A vector by introducing stop codons and removing 3’ Myc-DDK tag before being transferred to pINDUCER20 vector (adapter sequence is listed in Supplementary Table 15)
Showing 1-3 of 3 papers.
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