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PDZD3 (NM_024791) Human Tagged ORF Clone

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SKU
RC206415
PDZD3 (Myc-DDK-tagged)-Human PDZ domain containing 3 (PDZD3), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "PDZD3 or NM_024791"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PDZD3
Synonyms IKEPP; NHERF4; PDZK2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206415 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAAGCCGCAGATCTGCAGGACACAGCCTCGTTAACTCTGAAGTTTAAGTTTAACCCAAAGCTGG
GCATTGATAATCCTGTCCTCTCCCTGGCCGAAGACCACGACCCCTATGATCCCTGGAGCCTGGAGCGGCC
TCGCTTCTGTTTACTGAGCAAAGAGGAGGGCAAGAGTTTTGGCTTCCACCTGCAGCAGGAGCTGGGCAGG
GCTGGGCATGTGGTGTGCAGGGTGGACCCAGGCACCTCTGCCCAGCGCCAGGGTCTTCAGGAAGGAGACA
GGATCCTGGCGGTGAACAATGATGTTGTGGAACACGAAGACTATGCGGTGGTGGTACGCCGCATCCGGGC
CAGCAGCCCTCGGGTGTTGCTGACAGTATTGGCACGGCATGCACATGACGTGGCCCGAGCTCAGCTGGGA
GAAGATGCCCACCTCTGTCCCACCCTAGGCCCAGGGGTCCGGCCCCGGCTGTGCCACATAGTGAAAGATG
AGGGTGGTTTTGGCTTCAGTGTCACCCATGGCAATCAGGGTCCTTTCTGGTTGGTGCTAAGTACTGGAGG
AGCAGCTGAGCGGGCAGGGGTGCCCCCCGGGGCCCGGCTGCTGGAAGTGAATGGGCTTTGGCAGAGTGGA
CAGCAGGTGACCTTGCTGGTGGCAGGGCCAGAGGTGGAAGAACAGTGTCGCCAGCTGGGATTGCCCCTGG
CTGCACCCCTGGCAGAGGGCTGGGCACTGCCCACCAAGCCCCGCTGCCTGCACCTGGAGAAAGGGCCCCA
GGGTTTTGGGTTCCTGCTCCGGGAGGAAAAGGGCCTTGACGGTCGCCCTGGACAGTTCCTGTGGGAGGTG
GACCCGGGACTGCCAGCCAAGAAGGCTGGGATGCAGGCTGGGGACCGGCTGGTGGCTGTGGCTGGGGAGA
GCGTGGAGGGGCTGGGCCATGAGGAGACAGTGTCCAGGATCCAGGGGCAGGGCTCCTGTGTCTCCCTCAC
TGTCGTCGACCCTGAGGCGGACCGCTTCTTCAGCATGGTTCGCCTGTCCCCACTCCTCTTCTTGGAGAAC
ACAGAGGCTCCCGCCTCGCCCCAGGGCAGCAGCTCAGCCTCACTGGTTGAGACAGAGGACCCTTCACTTG
AAGACACAAGCGTGCCTTCTGTCCCTCTTGGCTCCCGACAGTGCTTCCTGTACCCTGGGCCTGGTGGCAG
CTATGGCTTCCGACTCAGTTGTGTGGCCAGTGGGCCTCGTCTCTTCATCTCCCAGGTGACTCCAGGAGGC
TCAGCTGCCCGGGCTGGGCTGCAAGTGGGAGACGTGATTCTGGAAGTGAACGGGTATCCTGTTGGGGGAC
AGAATGACCTGGAGAGGCTTCAGCAGCTGCCTGAGGCTGAGCCACCCCTCTGCCTGAAGCTGGCAGCCAG
GTCTCTGCGGGGCTTGGAAGCCTGGATTCCCCCTGGGGCTGCAGAGGACTGGGCTCTGGCCTCGGATCTA
CTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206415 protein sequence
Red=Cloning site Green=Tags(s)

MEKAADLQDTASLTLKFKFNPKLGIDNPVLSLAEDHDPYDPWSLERPRFCLLSKEEGKSFGFHLQQELGR
AGHVVCRVDPGTSAQRQGLQEGDRILAVNNDVVEHEDYAVVVRRIRASSPRVLLTVLARHAHDVARAQLG
EDAHLCPTLGPGVRPRLCHIVKDEGGFGFSVTHGNQGPFWLVLSTGGAAERAGVPPGARLLEVNGLWQSG
QQVTLLVAGPEVEEQCRQLGLPLAAPLAEGWALPTKPRCLHLEKGPQGFGFLLREEKGLDGRPGQFLWEV
DPGLPAKKAGMQAGDRLVAVAGESVEGLGHEETVSRIQGQGSCVSLTVVDPEADRFFSMVRLSPLLFLEN
TEAPASPQGSSSASLVETEDPSLEDTSVPSVPLGSRQCFLYPGPGGSYGFRLSCVASGPRLFISQVTPGG
SAARAGLQVGDVILEVNGYPVGGQNDLERLQQLPEAEPPLCLKLAARSLRGLEAWIPPGAAEDWALASDL
L

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024791
ORF Size 1473 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024791.2, NP_079067.2
RefSeq Size 2245 bp
RefSeq ORF 1476 bp
Locus ID 79849
UniProt ID Q86UT5, B0YJ61
Cytogenetics 11q23.3
Protein Families Druggable Genome
MW 52.5 kDa
Summary Guanylyl cyclase C (GCC, or GUCY2C; MIM 601330) produces cGMP following the binding of either endogenous ligands or heat-stable enterotoxins secreted by E. coli and other enteric bacteria. Activation of GCC initiates a signaling cascade that leads to phosphorylation of the cystic fibrosis transmembrane conductance regulator (CFTR; MIM 602421), followed by a net efflux of ions and water into the intestinal lumen. IKEPP is a regulatory protein that associates with GCC and regulates the amount of cGMP produced following receptor stimulation (Scott et al., 2002 PubMed 11950846).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_024791" in other vectors (7)
SKU Description Size Price
RC206415L1 Lenti ORF clone of Human PDZ domain containing 3 (PDZD3), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC206415L2 Lenti ORF clone of Human PDZ domain containing 3 (PDZD3), transcript variant 2, mGFP tagged 10 ug
$757.00
RC206415L3 Lenti ORF clone of Human PDZ domain containing 3 (PDZD3), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC206415L4 Lenti ORF clone of Human PDZ domain containing 3 (PDZD3), transcript variant 2, mGFP tagged 10 ug
$757.00
RG206415 PDZD3 (tGFP-tagged) - Human PDZ domain containing 3 (PDZD3), transcript variant 2 10 ug
$489.00 $657.00
SC122970 PDZD3 (untagged)-Human PDZ domain containing 3 (PDZD3), transcript variant 2 10 ug
$300.00
SC317823 PDZD3 (untagged)-Human PDZ domain containing 3 (PDZD3), transcript variant 2 10 ug
$503.00

Plasmid Map for RC206415

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206415
Circular map for RC206415

file-alt Citations

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