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PDZD3 (NM_024791) Human Untagged Clone

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SKU
SC317823
PDZD3 (untagged)-Human PDZ domain containing 3 (PDZD3), transcript variant 2
  $503.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PDZD3
Synonyms IKEPP; NHERF4; PDZK2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC317823 representing NM_024791.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGAGAAAGCCGCAGATCTGCAGGACACAGCCTCGTTAACTCTGAAGTTTAAGTTTAACCCAAAGCTG
GGCATTGATAATCCTGTCCTCTCCCTGGCCGAAGACCACGACCCCTATGATCCCTGGAGCCTGGAGCGG
CCTCGCTTCTGTTTACTGAGCAAAGAGGAGGGCAAGAGTTTTGGCTTCCACCTGCAGCAGGAGCTGGGC
AGGGCTGGGCATGTGGTGTGCAGGGTGGACCCAGGCACCTCTGCCCAGCGCCAGGGTCTTCAGGAAGGA
GACAGGATCCTGGCGGTGAACAATGATGTTGTGGAACACGAAGACTATGCGGTGGTGGTACGCCGCATC
CGGGCCAGCAGCCCTCGGGTGTTGCTGACAGTATTGGCACGGCATGCACATGACGTGGCCCGAGCTCAG
CTGGGAGAAGATGCCCACCTCTGTCCCACCCTAGGCCCAGGGGTCCGGCCCCGGCTGTGCCACATAGTG
AAAGATGAGGGTGGTTTTGGCTTCAGTGTCACCCATGGCAATCAGGGTCCTTTCTGGTTGGTGCTAAGT
ACTGGAGGAGCAGCTGAGCGGGCAGGGGTGCCCCCCGGGGCCCGGCTGCTGGAAGTGAATGGGCTTTGG
CAGAGTGGACAGCAGGTGACCTTGCTGGTGGCAGGGCCAGAGGTGGAAGAACAGTGTCGCCAGCTGGGA
TTGCCCCTGGCTGCACCCCTGGCAGAGGGCTGGGCACTGCCCACCAAGCCCCGCTGCCTGCACCTGGAG
AAAGGGCCCCAGGGTTTTGGGTTCCTGCTCCGGGAGGAAAAGGGCCTTGACGGTCGCCCTGGACAGTTC
CTGTGGGAGGTGGACCCGGGACTGCCAGCCAAGAAGGCTGGGATGCAGGCTGGGGACCGGCTGGTGGCT
GTGGCTGGGGAGAGCGTGGAGGGGCTGGGCCATGAGGAGACAGTGTCCAGGATCCAGGGGCAGGGCTCC
TGTGTCTCCCTCACTGTCGTCGACCCTGAGGCGGACCGCTTCTTCAGCATGGTTCGCCTGTCCCCACTC
CTCTTCTTGGAGAACACAGAGGCTCCCGCCTCGCCCCGGGGCAGCAGCTCAGCCTCACTGGTTGAGACA
GAGGACCCTTCACTTGAAGACACAAGCGTGCCTTCTGTCCCTCTTGGCTCCCGACAGTGCTTCCTGTAC
CCTGGGCCTGGTGGCAGCTATGGCTTCCGACTCAGTTGTGTGGCCAGTGGGCCTCGTCTCTTCATCTCC
CAGGTGACTCCAGGAGGCTCAGCTGCCCGGGCTGGGCTGCAAGTGGGAGACGTGATTCTGGAAGTGAAC
GGGTATCCTGTTGGGGGACAGAATGACCTGGAGAGGCTTCAGCAGCTGCCTGAGGCTGAGCCACCCCTC
TGCCTGAAGCTGGCAGCCAGGTCTCTGCGGGGCTTGGAAGCCTGGATTCCCCCTGGGGCTGCAGAGGAC
TGGGCTCTGGCCTCGGATCTACTGTAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_024791
Insert Size 1476 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024791.3
RefSeq Size 2245 bp
RefSeq ORF 1476 bp
Locus ID 79849
UniProt ID Q86UT5
Cytogenetics 11q23.3
Protein Families Druggable Genome
MW 52.5 kDa
Summary Guanylyl cyclase C (GCC, or GUCY2C; MIM 601330) produces cGMP following the binding of either endogenous ligands or heat-stable enterotoxins secreted by E. coli and other enteric bacteria. Activation of GCC initiates a signaling cascade that leads to phosphorylation of the cystic fibrosis transmembrane conductance regulator (CFTR; MIM 602421), followed by a net efflux of ions and water into the intestinal lumen. IKEPP is a regulatory protein that associates with GCC and regulates the amount of cGMP produced following receptor stimulation (Scott et al., 2002 PubMed 11950846).supplied by OMIM, Mar 2008
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.
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Documents
Resources
"NM_024791" in other vectors (7)
SKU Description Size Price
RC206415 PDZD3 (Myc-DDK-tagged)-Human PDZ domain containing 3 (PDZD3), transcript variant 2 10 ug
$457.00
RC206415L1 Lenti ORF clone of Human PDZ domain containing 3 (PDZD3), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC206415L2 Lenti ORF clone of Human PDZ domain containing 3 (PDZD3), transcript variant 2, mGFP tagged 10 ug
$757.00
RC206415L3 Lenti ORF clone of Human PDZ domain containing 3 (PDZD3), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC206415L4 Lenti ORF clone of Human PDZ domain containing 3 (PDZD3), transcript variant 2, mGFP tagged 10 ug
$757.00
RG206415 PDZD3 (tGFP-tagged) - Human PDZ domain containing 3 (PDZD3), transcript variant 2 10 ug
$489.00 $657.00
SC122970 PDZD3 (untagged)-Human PDZ domain containing 3 (PDZD3), transcript variant 2 10 ug
$300.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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