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Ensconsin (MAP7) (NM_003980) Human Tagged ORF Clone

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SKU
RC206388
MAP7 (Myc-DDK-tagged)-Human microtubule-associated protein 7 (MAP7), transcript variant 4
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $686.00
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Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Ensconsin
Synonyms E-MAP-115; EMAP115
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206388 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGCTAGGAGCTGGCGGCGACGGCCACAGGGGCGGCGACGGCGCAGTGCGAAGCGAAACAGCAC
CCGACAGCTACAAAGTGCAAGATAAGAAAAATGCCTCCAGCCGCCCTGCCTCTGCAATTTCAGGACAAAA
TAACAACCACTCAGGAAATAAACCAGACCCTCCGCCTGTGTTACGTGTTGATGACCGGCAGCGGCTGGCC
CGGGAGCGACGTGAGGAACGGGAGAAACAGCTAGCTGCAAGAGAAATAGTGTGGTTAGAAAGAGAAGAGC
GAGCCAGGCAGCACTACGAGAAGCACCTGGAAGAGCGGAAGAAGAGGTTGGAGGAGCAGAGGCAGAAGGA
GGAGCGGAGGAGGGCTGCTGTGGAGGAGAAGCGGAGGCAGAGACTTGAGGAGGACAAAGAACGCCACGAA
GCTGTTGTACGGCGCACAATGGAAAGGAGCCAGAAGCCAAAACAGAAGCATAACCGTTGGTCGTGGGGAG
GCTCTCTCCATGGGAGCCCTAGCATCCACAGTGCAGATCCAGACAGGCGGTCAGTTTCCACCATGAATCT
TTCGAAATATGTTGATCCCGTCATTAGCAAGCGGCTCTCCTCTTCATCTGCAACTTTACTAAATTCTCCA
GATAGAGCTCGCCGCCTGCAGCTCAGCCCATGGGAGAGCAGCGTTGTTAACAGACTCCTGACGCCCACAC
ATTCGTTCCTGGCCAGAAGTAAAAGCACAGCTGCCTTGTCTGGAGAAGCAGCATCTTGCAGCCCCATCAT
CATGCCCTACAAAGCTGCACACTCTAGAAATTCGATGGATCGACCAAAACTCTTTGTAACACCACCTGAG
GGCTCTTCTCGCAGGAGGATCATTCATGGCACAGCGAGCTATAAAAAAGAAAGAGAGAGAGAAAATGTAC
TCTTCCTCACATCTGGCACCCGAAGGGCTGTATCTCCATCTAATCCCAAAGCAAGACAACCAGCTCGCTC
CCGACTTTGGCTTCCGTCCAAGTCTCTTCCTCATTTGCCTGGCACACCCAGACCGACATCCTCCTTGCCA
CCCGGCTCAGTCAAAGCTGCTCCTGCTCAGGTCCGGCCCCCATCCCCCGGCAACATCCGCCCTGTCAAGA
GGGAAGTCAAAGTGGAGCCTGAGAAGAAAGATCCTGAGAAGGAACCTCAGAAAGTTGCCAATGAGCCCTC
ACTAAAGGGCAGAGCACCTTTAGTGAAGGTAGAAGAAGCCACAGTTGAAGAGCGGACACCTGCTGAACCA
GAAGTTGGCCCTGCTGCTCCAGCCATGGCCCCAGCTCCAGCCTCGGCCCCAGCTCCAGCCTCGGCCCCAG
CTCCAGCCCCGGTCCCCACCCCAGCCATGGTCTCAGCCCCGTCATCCACTGTGAATGCCAGTGCTTCTGT
TAAGACTTCTGCAGGCACCACCGACCCAGAGGAGGCCACAAGGCTTCTAGCTGAGAAGAGGCGGCTGGCC
CGAGAGCAGAGAGAAAAGGAAGAAAGGGAGAGGAGGGAGCAGGAAGAGCTTGAAAGACAAAAGAGAGAGG
AATTGGCTCAACGTGTGGCTGAAGAGAGGACGACTCGCCGTGAGGAGGAGTCGCGCAGGCTGGAAGCCGA
GCAGGCCCGGGAGAAGGAGGAGCAGCTGCAGCGGCAGGCGGAGGAGCGGGCGCTGCGCGAGTGGGAGGAG
GCAGAGCGCGCCCAGAGGCAGAAAGAAGAAGAAGCTCGCGTTCGTGAAGAAGCAGAGAGGGTCCGGCAGG
AACGAGAGAAGCATTTCCAGAGAGAAGAGCAAGAGCGCCTGGAGAGAAAGAAGCGACTTGAGGAGATTAT
GAAAAGAACCAGGAGAACAGAAGCTACAGATAAGAAAACCAGTGATCAGAGAAACGGTGATATAGCCAAG
GGAGCTCTCACTGGAGGAACAGAGGTGTCTGCACTTCCATGTACAACAAACGCTCCGGGAAATGGAAAGC
CAGTTGGTAGCCCACATGTGGTTACCTCACACCAGTCAAAAGTGACAGTGGAGAGCACTCCCGATTTGGA
AAAACAACCAAATGAAAATGGTGTATCTGTTCAGAATGAAAATTTTGAAGAAATTATAAACTTACCCATT
GGATCTAAACCATCCAGATTAGATGTCACCAACAGTGAGAGCCCAGAAATTCCTTTGAATCCAATTTTGG
CCTTTGATGATGAAGGGACACTTGGGCCCCTGCCTCAGGTAGATGGTGTTCAGACACAGCAGACTGCAGA
AGTTATA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206388 protein sequence
Red=Cloning site Green=Tags(s)

MAELGAGGDGHRGGDGAVRSETAPDSYKVQDKKNASSRPASAISGQNNNHSGNKPDPPPVLRVDDRQRLA
RERREEREKQLAAREIVWLEREERARQHYEKHLEERKKRLEEQRQKEERRRAAVEEKRRQRLEEDKERHE
AVVRRTMERSQKPKQKHNRWSWGGSLHGSPSIHSADPDRRSVSTMNLSKYVDPVISKRLSSSSATLLNSP
DRARRLQLSPWESSVVNRLLTPTHSFLARSKSTAALSGEAASCSPIIMPYKAAHSRNSMDRPKLFVTPPE
GSSRRRIIHGTASYKKERERENVLFLTSGTRRAVSPSNPKARQPARSRLWLPSKSLPHLPGTPRPTSSLP
PGSVKAAPAQVRPPSPGNIRPVKREVKVEPEKKDPEKEPQKVANEPSLKGRAPLVKVEEATVEERTPAEP
EVGPAAPAMAPAPASAPAPASAPAPAPVPTPAMVSAPSSTVNASASVKTSAGTTDPEEATRLLAEKRRLA
REQREKEERERREQEELERQKREELAQRVAEERTTRREEESRRLEAEQAREKEEQLQRQAEERALREWEE
AERAQRQKEEEARVREEAERVRQEREKHFQREEQERLERKKRLEEIMKRTRRTEATDKKTSDQRNGDIAK
GALTGGTEVSALPCTTNAPGNGKPVGSPHVVTSHQSKVTVESTPDLEKQPNENGVSVQNENFEEIINLPI
GSKPSRLDVTNSESPEIPLNPILAFDDEGTLGPLPQVDGVQTQQTAEVI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003980
ORF Size 2247 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003980.4
RefSeq Size 4608 bp
RefSeq ORF 2250 bp
Locus ID 9053
UniProt ID Q14244
Cytogenetics 6q23.3
Protein Families Druggable Genome
MW 84.1 kDa
Summary The product of this gene is a microtubule-associated protein that is predominantly expressed in cells of epithelial origin. Microtubule-associated proteins are thought to be involved in microtubule dynamics, which is essential for cell polarization and differentiation. This protein has been shown to be able to stabilize microtubules, and may serve to modulate microtubule functions. Studies of the related mouse protein also suggested an essential role in microtubule function required for spermatogenesis. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Oct 2010
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Documents
Resources
"NM_003980" in other vectors (6)
SKU Description Size Price
RC206388L1 Lenti ORF clone of Human microtubule-associated protein 7 (MAP7), transcript variant 4, Myc-DDK-tagged 10 ug
$986.00
RC206388L2 Lenti ORF clone of Human microtubule-associated protein 7 (MAP7), transcript variant 4, mGFP tagged 10 ug
$986.00
RC206388L3 Lenti ORF clone of Human microtubule-associated protein 7 (MAP7), transcript variant 4, Myc-DDK-tagged 10 ug
$986.00
RC206388L4 Lenti ORF clone of Human microtubule-associated protein 7 (MAP7), transcript variant 4, mGFP tagged 10 ug
$986.00
RG206388 MAP7 (tGFP-tagged) - Human microtubule-associated protein 7 (MAP7), transcript variant 4 10 ug
$886.00
SC322593 MAP7 (untagged)-Human microtubule-associated protein 7 (MAP7), transcript variant 4 10 ug
$687.00

Plasmid Map for RC206388

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206388
Circular map for RC206388

file-alt Citations

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