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Ensconsin (MAP7) (NM_003980) Human Untagged Clone

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SKU
SC322593
MAP7 (untagged)-Human microtubule-associated protein 7 (MAP7), transcript variant 4
  $687.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Ensconsin
Synonyms E-MAP-115; EMAP115
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for SC322593 GAGCACCATGGCGGAGCTAGGAGCTGGCGGCGACGGCCACAGGGGCGGCGACGGCGCAGT
GCGAAGCGAAACAGCACCCGACAGCTACAAAGTGCAAGATAAGAAAAATGCCTCCAGCCG
CCCTGCCTCTGCAATTTCAGGACAAAATAACAACCACTCAGGAAATAAACCAGACCCTCC
GCCTGTGTTACGTGTTGATGACCGGCAGCGGCTGGCCCGGGAGCGACGTGAGGAACGGGA
GAAACAGCTAGCTGCAAGAGAAATAGTGTGGTTAGAAAGAGAAGAGCGAGCCAGGCAGCA
CTACGAGAAGCACCTGGAAGAGCGGAAGAAGAGGTTGGAGGAGCAGAGGCAGAAGGAGGA
GCGGAGGAGGGCTGCTGTGGAGGAGAAGCGGAGGCAGAGACTTGAGGAGGACAAAGAACG
CCACGAAGCTGTTGTACGGCGCACAATGGAAAGGAGCCAGAAGCCAAAACAGAAGCATAA
CCGTTGGTCGTGGGGAGGCTCTCTCCATGGGAGCCCTAGCATCCACAGTGCAGATCCAGA
CAGGCGGTCAGTTTCCACCATGAATCTTTCGAAATATGTTGATCCCGTCATTAGCAAGCG
GCTCTCCTCTTCATCTGCAACTTTACTAAATTCTCCAGATAGAGCTCGCCGCCTGCAGCT
CAGCCCATGGGAGAGCAGCGTTGTTAACAGACTCCTGACGCCCACACATTCGTTCCTGGC
CAGAAGTAAAAGCACAGCTGCCTTGTCTGGAGAAGCAGCATCTTGCAGCCCCATCATCAT
GCCCTACAAAGCTGCACACTCTAGAAATTCGATGGATCGACCAAAACTCTTTGTAACACC
ACCTGAGGGCTCTTCTCGCAGGAGGATCATTCATGGCACAGCGAGCTATAAAAAAGAAAG
AGAGAGAGAAAATGTACTCTTCCTCACATCTGGCACCCGAAGGGCTGTATCTCCATCTAA
TCCCAAAGCAAGACAACCAGCTCGCTCCCGACTTTGGCTTCCGTCCAAGTCTCTTCCTCA
TTTGCCTGGCACACCCAGACCGACATCCTCCTTGCCACCCGGCTCAGTCAAAGCTGCTCC
TGCTCAGGTCCGGCCCCCATCCCCCGGCAACATCCGCCCTGTCAAGAGGGAAGTCAAAGT
GGAGCCTGAGAAGAAAGATCCTGAGAAGGAACCTCAGAAAGTTGCCAATGAGCCCTCACT
AAAGGGCAGAGCACCTTTAGTGAAGGTAGAAGAAGCCACAGTTGAAGAGCGGACACCTGC
TGAACCAGAAGTTGGCCCTGCTGCTCCAGCCATGGCCCCAGCTCCAGCCTCGGCCCCAGC
TCCAGCCTCGGCCCCAGCTCCAGCCCCGGTCCCCACCCCAGCCATGGTCTCAGCCCCGTC
ATCCACTGTGAATGCCAGTGCTTCTGTTAAGACTTCTGCAGGCACCACCGACCCAGAGGA
GGCCACAAGGCTTCTAGCTGAGAAGAGGCGGCTGGCCCGAGAGCAGAGAGAAAAGGAAGA
AAGGGAGAGGAGGGAGCAGGAAGAGCTTGAAAGACAAAAGAGAGAGGAATTGGCTCAACG
TGTGGCTGAAGAGAGGACGACTCGCCGTGAGGAGGAGTCGCGCAGGCTGGAAGCCGAGCA
GGCCCGGGAGAAGGAGGAGCAGCTGCAGCGGCAGGCGGAGGAGCGGGCGCTGCGCGAGTG
GGAGGAGGCAGAGCGCGCCCAGAGGCAGAAAGAAGAAGAAGCTCGCGTTCGTGAAGAAGC
AGAGAGGGTCCGGCAGGAACGAGAGAAGCATTTCCAGAGAGAAGAGCAAGAGCGCCTGGA
GAGAAAGAAGCGACTTGAGGAGATTATGAAAAGAACCAGGAGAACAGAAGCTACAGATAA
GAAAACCAGTGATCAGAGAAACGGTGATATAGCCAAGGGAGCTCTCACTGGAGGAACAGA
GGTGTCTGCACTTCCATGTACAACAAACGCTCCGGGAAATGGAAAGCCAGTTGGTAGCCC
ACATGTGGTTACCTCACACCAGTCAAAAGTGACAGTGGAGAGCACTCCCGATTTGGAAAA
ACAACCAAATGAAAATGGTGTATCTGTTCAGAATGAAAATTTTGAAGAAATTATAAACTT
ACCCATTGGATCTAAACCATCCAGATTAGATGTCACCAACAGTGAGAGCCCAGAAATTCC
TTTGAATCCAATTTTGGCCTTTGATGATGAAGGGACACTTGGGCCCCTGCCTCAGGTAGA
TGGTGTTCAGACACAGCAGACTGCAGAAGTTATATGAGTGTTTCTTCTGAAGAACCAAAG
CTGAAATTTAATGAGAATTTCTACAATTAATGGAATTCCTTTCCTGCTATAAAGGAGCAT
CCCCTCCACCCGTTTTCTAGAGTTCTTGACCATCATTTTGAAAAGATTTATTAAAACTAG
CTAAAGACAACAGACTGGATAGCTTTTCTAATAATTTTCATCAATAGGAAAAAAGAAATA
CGTCTCATTCTTCAATACTTTAAAATGGCTTTTTCCAGTGTGCTCCTTCTTAGCAATCAA
TATTTTTCTGCATTCTTTAAAAGACAAGAGAATTTGGTTATAAAAGAAATGGGCTGACTA
GGCATGATTTTTTTGGTCTTAAAAGCTTAACATGTAAAATTGGCAAAAAAAATTTTTTAC
CTTTTATAATACTTGAAAAATAAGTACCTCTTTGTTCTACAAGTAGAATGAATAGGAGAA
GAGTTTAAGCCTGTTTTTTTAAAATATTATTGCAAAGAGCTCTATTTGTAGAAGCAAATT
ATAGGCAGATTACCAGGTTCTTATAAATACAGCTTGTACATGGACATTCTGCAAACCCAG
CTGTCACATTTTTCTTGCAACTCCTTTTGCAAAAGCAGACTAAAATGTTTTAAAATGTGA
AAAAACATTATTTTTTCAAAGCAAGAAAATATATAAAGTTTTTCCAGATAAACTAATCAA
ATAAATTAGAATAATGTGACAACATTACAAATTTAATTTGTTAGCTGCATTCCTTCTGAT
GTTACCACGATAGAATGTTACTGATGATTCAGGGCTATTTCTGAAGTCTGTATGTTGCTG
CTGTCCCCAGTGATGGTGGACTTATCTTTGCCTTACCTGATCACAAATTATGTTGGGGAA
AATAAAGATTTAATATTTCTTTAAATAGAAAAAGAATTTGGTTTTGCTCGTTTAAGAGCA
ATGAGAAAATGATGGAATGTTGACTGTGTTTGGCACACAGGACACGGACCTTCATGGAAG
TCCTTGCTCTGCGTGGCATCTGTCAGCTTTTCACCTTTCATTCTTATTCTTCACTTTTGC
TGCTGAGCCTAGCTGTACAAACTTGCACTTTCATTTGCTAATATAAATTCAGTTTTATTT
TACCATTTTAGAGACTACTAATGATTAAATGTAGAAGGAGAGGGTGCACATGTTTTTATG
TGGAGTGTTTAAAAGATAAATTTATACCACTGTAATGTGCAGCTTTTATTAAAAGAGAAA
TTGGTTGAACTGCTAGGTTGAATGAGAGACTTCATCTATTGGACTATTTTTTTTAATCCA
GGCATATGGTCTTTAGTAATGGCTTGTAATTTGTGAAAACATTAATTTGGGGGTTTTCCC
TGTTTTCAGTTGTCCATGTACACATAGTCATTATATTAGAAAAGAAAGCTGTTCAACAAA
CTTGTTTAATTTGTTTAAATCAACATAGCATGAAACACCAAATAAAATGTTTGACATAGT
TTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_003980
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003980.3, NP_003971.1
RefSeq Size 4018 bp
RefSeq ORF 2250 bp
Locus ID 9053
UniProt ID Q14244
Cytogenetics 6q23.3
Protein Families Druggable Genome
Summary The product of this gene is a microtubule-associated protein that is predominantly expressed in cells of epithelial origin. Microtubule-associated proteins are thought to be involved in microtubule dynamics, which is essential for cell polarization and differentiation. This protein has been shown to be able to stabilize microtubules, and may serve to modulate microtubule functions. Studies of the related mouse protein also suggested an essential role in microtubule function required for spermatogenesis. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Oct 2010
Transcript Variant: This variant (4) has an alternate 5' exon, as compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus, as compared to isoform 1.
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Documents
Resources
"NM_003980" in other vectors (6)
SKU Description Size Price
RC206388 MAP7 (Myc-DDK-tagged)-Human microtubule-associated protein 7 (MAP7), transcript variant 4 10 ug
$686.00
RC206388L1 Lenti ORF clone of Human microtubule-associated protein 7 (MAP7), transcript variant 4, Myc-DDK-tagged 10 ug
$986.00
RC206388L2 Lenti ORF clone of Human microtubule-associated protein 7 (MAP7), transcript variant 4, mGFP tagged 10 ug
$986.00
RC206388L3 Lenti ORF clone of Human microtubule-associated protein 7 (MAP7), transcript variant 4, Myc-DDK-tagged 10 ug
$986.00
RC206388L4 Lenti ORF clone of Human microtubule-associated protein 7 (MAP7), transcript variant 4, mGFP tagged 10 ug
$986.00
RG206388 MAP7 (tGFP-tagged) - Human microtubule-associated protein 7 (MAP7), transcript variant 4 10 ug
$886.00

file-alt Citations

Quantitative Mass Spectrometry Identifies Novel Host Binding Partners for Pathogenic Escherichia coli Type III Secretion System Effectors
Law, Law, Scurll et al
J. Proteome Res. (2016)
Cited by 12
AI Summary: OriGene’s human cDNA clone SC322593 was used as the template to amplify the map7 gene, enabling the construction of MAP7 expression plasmids that were essential for identifying and validating host protein interactions with E. coli effectors in this study.
Snippet: The map7 gene sequence was amplified from human cDNA clone SC322593 (OriGene Technologies, Inc.) and cloned into pCMVTag4A (Stratagene) to generate pmap7-FLAG. MAP7 truncations were amplified using pmap7-FLAG as a template and inserted into pCMVTag4A
Showing 1-1 of 1 papers.
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