PDGF Receptor beta (PDGFRB) (NM_002609) Human Tagged ORF Clone
- TrueORF®
SKU
RC206377
PDGFRB (Myc-DDK-tagged)-Human platelet-derived growth factor receptor, beta polypeptide (PDGFRB)
$1,444.00
5 Days*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PDGF Receptor beta |
| Synonyms | CD140B; IBGC4; IMF1; JTK12; KOGS; PDGFR; PDGFR-1; PDGFR1; PENTT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002609 |
| ORF Size | 3318 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002609.4 |
| RefSeq Size | 5718 bp |
| RefSeq ORF | 3321 bp |
| Locus ID | 5159 |
| UniProt ID | P09619 |
| Cytogenetics | 5q32 |
| Domains | ig, IG, IGc2, pkinase, S_TKc, TyrKc |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane |
| Protein Pathways | Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton |
| MW | 123.8 kDa |
| Summary | The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This gene is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the ETV6 gene, results in chronic myeloproliferative disorder with eosinophilia. provided by RefSeq, Aug 2017 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002609" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC206377L1 | Lenti ORF clone of Human platelet-derived growth factor receptor, beta polypeptide (PDGFRB), Myc-DDK-tagged | 10 ug |
$1,744.00
|
|
| RC206377L2 | Lenti ORF clone of Human platelet-derived growth factor receptor, beta polypeptide (PDGFRB), mGFP tagged | 10 ug |
$1,744.00
|
|
| RC206377L3 | Lenti ORF clone of Human platelet-derived growth factor receptor, beta polypeptide (PDGFRB), Myc-DDK-tagged | 10 ug |
$1,744.00
|
|
| RC206377L4 | Lenti ORF clone of Human platelet-derived growth factor receptor, beta polypeptide (PDGFRB), mGFP tagged | 10 ug |
$1,744.00
|
|
| RC600017 | PDGFRB (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens platelet-derived growth factor receptor, beta polypeptide, Signal peptide (1-32) plus EC domain (33-532) | 10 ug |
$743.00
|
|
| RG206377 | PDGFRB (tGFP-tagged) - Human platelet-derived growth factor receptor, beta polypeptide (PDGFRB) | 10 ug |
$789.00
$1,644.00
|
|
| SC309979 | PDGFRB (untagged)-Human platelet-derived growth factor receptor, beta polypeptide (PDGFRB) | 10 ug |
$1,445.00
|
|
Citations
LncRNA Hmrhl regulates expression of cancer related genes in chronic myelogenous leukemia through chromatin association
Choudhury, Dutta, Bhaduri et al
NAR Cancer (2021) 3 (4), zcab042 DOI: 10.1093/narcan/zcab042
Choudhury, Dutta, Bhaduri et al
NAR Cancer (2021) 3 (4), zcab042 DOI: 10.1093/narcan/zcab042
Cited by 14
AI Summary: In this study, OriGene products—specifically the Hmrhl shRNA (OriGene, Cat# TL320691) and TAL1 overexpression plasmid (OriGene, Cat# RC200446)—were used for gene silencing and overexpression experiments to investigate the functional role of Hmrhl lncRNA and its regulation in chronic myelogenous leukemia cells.
P68 RNA Helicase facilitates Breast Cancer progression by promoting Proliferation and Migration via PDGFR-β/AR axis
Panchbhai, Turaga, Sharma et al
J. Cancer (2021) 12 (21), 6543-6552 DOI: 10.7150/jca.61505
Panchbhai, Turaga, Sharma et al
J. Cancer (2021) 12 (21), 6543-6552 DOI: 10.7150/jca.61505
Cited by 2
AI Summary: OriGene’s PDGFR-β plasmid (RC206377) was used to overexpress PDGFR-β in breast cancer cell lines, enabling the study of its role in proliferation and migration in this research.
Snippet: To overexpress PDGFR−β in MDAMB231 and BT549 cells, 2.5 µg of PDGFR-β plasmid (Origene RC206377) was transfected for 48 h using Lipofectamine 3000 Transfection Reagent kit (Thermo Fisher scientific). Immunoblotting
P68 RNA Helicase facilitates Breast Cancer progression by promoting Proliferation and Migration via PDGFR-β/AR axis
Panchbhai, Turaga, Sharma et al
J Cancer (2021) 12 (21), 6543-6552 DOI: 10.7150/jca.61505
Panchbhai, Turaga, Sharma et al
J Cancer (2021) 12 (21), 6543-6552 DOI: 10.7150/jca.61505
Cited by 2
AI Summary: In this study, OriGene’s human DDX5 (p68) shRNA lentiviral particles and PDGFRB (PDGFR-β) antibody were used to knockdown p68 expression and detect PDGFR-β protein levels, respectively, enabling the authors to elucidate the role of p68 in promoting breast cancer progression via the PDGFR-β/AR axis.
RNA HELICASE DDX5 REGULATES PLATELET DERIVED GROWTH FACTOR RECEPTORAND ANDROGEN RECEPTOR (AR) EXPRESSION IN BREAST CANCER
Panchbhai
Thesis (2020)
Panchbhai
Thesis (2020)
Snippet: To knock down p68, PDGFR β, in MDA MB 231 and BT549 cells, si RNA p68 (Santa cruz sc37141) or si RNA PDGFR β (ScBT- sc29442) respectively was transfected with 20–50 nM of siRNAs using Lipofectamine RNAiMAX Transfection Reagent kit from Thermo Fisher scientific. To overexpress PDGFRβ in MDA MB 231 and BT549 cells, 2.5 µg of PDGFRβ plasmid (Origene - RC206377) Lipofectamine 3000 Transfection Reagent kit from
Analysis of gene expression and functional characterization of XPR1: a pathogenic gene for primary familial brain calcification
Yao, Zhao, Wang et al
Cell Tissue Res. (2017)
Yao, Zhao, Wang et al
Cell Tissue Res. (2017)
Showing 1-5 of 5 papers.
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