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ZNF431 (NM_133473) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC206349
ZNF431 (Myc-DDK-tagged)-Human zinc finger protein 431 (ZNF431). Note: ORF is codon optimized
ORF Plasmid:
DDK tGFP
AAV Particle:
DDK
$289.00 $536.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZNF431
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206349 ORF sequence, codon optimized.
Due to the complexity of NM_133473, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGATCTGAAGTATGGGGTATACCCACTCAAAGAGGCTAGCGGGTGTCCGGGGGCCGAAAGGAATC
TTCTGGTGTACTCCTACTTCGAGAAGGAGACTCTCACTTTCAGGGATGTGGCAATTGAATTTTCTTTGGA
GGAGTGGGAATGTCTGAATCCCGCCCAGCAGAATCTGTATATGAATGTTATGTTGGAGAATTACAAGAAT
TTGGTGTTCCTCGGAGTGGCTGTCAGCAAGCAAGACCCCGTGACATGCCTGGAACAGGAGAAGGAACCCT
GGAATATGAAGAGACACGAAATGGTCGACGAACCCCCTGCTATGTGTTCATACTTCACCAAGGATCTTTG
GCCCGAACAAGACATTAAGGACAGCTTCCAGCAAGTAATCCTGCGGAGGTATGGGAAGTGCGAGCATGAA
AATCTTCAGTTGCGGAAAGGTTCCGCTTCCGTGGACGAGTACAAAGTACACAAGGAGGGGTACAACGAAC
TGAACCAATGCCTGACAACTACTCAGTCAAAGATTTTTCCATGCGATAAGTATGTTAAAGTCTTTCATAA
ATTCCTGAATGCGAATAGGCATAAGACAAGGCATACGGGTAAAAAGCCTTTCAAGTGCAAAAAATGTGGA
AAATCTTTCTGTATGCTCCTGCACCTCAGTCAGCACAAGAGGATTCATATCAGGGAGAACTCATACCAGT
GCGAGGAGTGCGGAAAGGCATTTAAGTGGTTTTCCACTCTTACCAGACACAAAAGGATACATACTGGAGA
AAAGCCCTTTAAGTGCGAGGAGTGCGGAAAGGCCTTTAAACAGTCCTCCACATTGACCACTCACAAAATT
ATTCATACAGGGGAAAAACCCTACAGGTGCGAGGAATGTGGGAAGGCCTTTAATAGAAGTTCTCATCTGA
CCACACATAAGATTATCCACACTGGAGAGAAACCCTACAAATGCGAGGAATGTGGAAAAGCTTTTAACCA
GTCCTCTACCCTGAGCACCCATAAATTCATACACGCTGGAGAGAAACCATACAAGTGCGAAGAGTGTGAT
AAAGCTTTCAACAGATTTAGCTACCTCACCAAGCATAAGATCATCCACACTGGAGAAAAGTCCTATAAGT
GCGAAGAGTGTGGGAAGGGGTTCAATTGGAGTTCTACCCTCACCAAGCATAAGCGAATTCACACGGGGGA
GAAGCCATACAAGTGTGAGGTATGTGGCAAGGCTTTTAATGAGAGCAGTAACCTGACCACACACAAGATG
ATTCACACCGGAGAAAAACCATACAAATGCGAGGAGTGTGGGAAAGCTTTCAACAGGAGCCCTCAGTTGA
CCGCGCATAAGATTATTCATACCGGCGAGAAGCCTTACAAATGCGAGGAGTGTGGAAAAGCTTTTAGCCA
GTCTTCAATCCTGACCACACATAAGAGAATTCACACCGGAGAAAAGCCTTATAAGTGTGAAGAGTGTGGG
AAAGCTTTCAATCGGTCATCTAACCTGACTAAACACAAAATTATCCATACCGGTGAGAAGTCATATAAGT
GCGAGGAGTGCGGCAAGGCCTTTAACCAATCTAGTACTCTTACTAAGCATCGGAAAATCCACACACGCCA
GAAGCCATATAATTGCGAAGAGTGCGATAACACTTTTAACCAGTCAAGCAACCTCATTAAGCAGAATAAT
TCTTATTGGCGGGAGACATTGCAGATGAGCCGAATGTGGGAATCACTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206349 representing NM_133473
Red=Cloning site Green=Tags(s)

MDDLKYGVYPLKEASGCPGAERNLLVYSYFEKETLTFRDVAIEFSLEEWECLNPAQQNLYMNVMLENYKN
LVFLGVAVSKQDPVTCLEQEKEPWNMKRHEMVDEPPAMCSYFTKDLWPEQDIKDSFQQVILRRYGKCEHE
NLQLRKGSASVDEYKVHKEGYNELNQCLTTTQSKIFPCDKYVKVFHKFLNANRHKTRHTGKKPFKCKKCG
KSFCMLLHLSQHKRIHIRENSYQCEECGKAFKWFSTLTRHKRIHTGEKPFKCEECGKAFKQSSTLTTHKI
IHTGEKPYRCEECGKAFNRSSHLTTHKIIHTGEKPYKCEECGKAFNQSSTLSTHKFIHAGEKPYKCEECD
KAFNRFSYLTKHKIIHTGEKSYKCEECGKGFNWSSTLTKHKRIHTGEKPYKCEVCGKAFNESSNLTTHKM
IHTGEKPYKCEECGKAFNRSPQLTAHKIIHTGEKPYKCEECGKAFSQSSILTTHKRIHTGEKPYKCEECG
KAFNRSSNLTKHKIIHTGEKSYKCEECGKAFNQSSTLTKHRKIHTRQKPYNCEECDNTFNQSSNLIKQNN
SYWRETLQMSRMWESL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133473
ORF Size 1728 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133473.1, NM_133473.2, NM_133473.3, NP_597730.1, NP_597730.2
RefSeq Size 13894 bp
RefSeq ORF 1731 bp
Locus ID 170959
UniProt ID Q8TF32
Cytogenetics 19p12
MW 67.2 kDa
Summary This gene encodes a member of the Krueppel C2H2-type zinc-finger family of proteins. The encoded protein may negatively regulate transcription of target genes, including the hedgehog signaling pathway receptor patched 1, by interacting with histone deacetylases. Mutations in this gene may be associated with non-syndromic facial clefting in human patients. provided by RefSeq, Jul 2016
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"NM_133473" in other vectors (2)
SKU Description Size Price
RG206349 ZNF431 (tGFP-tagged) - Human zinc finger protein 431 (ZNF431). Note: ORF is codon optimized 10 ug
$736.00
SC305963 ZNF431 (untagged)-Human zinc finger protein 431 (ZNF431) 10 ug
$591.00

Plasmid Map for RC206349

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206349
Circular map for RC206349

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