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SECISBP2 (NM_024077) Human Tagged ORF Clone

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SKU
RC206346
SECISBP2 (Myc-DDK-tagged)-Human SECIS binding protein 2 (SECISBP2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $782.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SECISBP2
Synonyms SBP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206346 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCGGAGGGGCCGCGGGAGCCCGAAAGCGAGGGCATCAAGTTATCAGCAGATGTCAAACCATTTG
TCCCCAGATTTGCCGGGCTCAATGTGGCATGGTTAGAGTCCTCAGAAGCATGTGTCTTCCCCAGCTCTGC
AGCCACATACTATCCGTTTGTTCAGGAACCACCAGTGACAGAGCAGAAAATATATACTGAAGACATGGCC
TTTGGAGCTTCAACTTTTCCACCTCAGTATTTATCTTCTGAGATAACTCTTCATCCATATGCCTATTCTC
CTTATACCCTTGACTCCACACAGAATGTTTACTCAGTGCCTGGCTCCCAGTATCTTTATAACCAACCCAG
TTGTTACCGAGGTTTTCAAACAGTGAAGCATCGAAATGAGAACACATGCCCTCTCCCACAAGAAATGAAA
GCTCTGTTTAAGAAGAAAACCTATGATGAGAAAAAAACGTATGATCAGCAAAAGTTTGACAGTGAAAGGG
CTGATGGAACTATATCATCTGAGATAAAATCAGCTAGAGGTTCACATCATTTGTCCATTTACGCTGAGAA
TAGTTTGAAATCAGATGGTTACCATAAGCGAACAGACAGGAAATCCAGAATCATTGCAAAAAATGTATCT
ACCTCCAAACCTGAGTTTGAATTTACCACACTGGACTTTCCTGAACTGCAAGGTGCAGAGAACAATATGT
CAGAGATACAGAAGCAACCCAAGTGGGGACCTGTCCACTCTGTCTCTACCGACATTTCTCTTCTAAGAGA
AGTAGTAAAACCAGCTGCAGTGTTATCAAAGGGTGAAATAGTGGTGAAAAATAACCCAAATGAATCTGTA
ACTGCTAATGCCGCTACCAATTCTCCTTCATGTACAAGAGAGTTATCTTGGACACCAATGGGTTATGTTG
TTCGACAGACATTATCTACAGAACTGTCAGCAGCCCCTAAAAATGTTACTTCTATGATAAACTTAAAGAC
CATTGCTTCATCAGCAGATCCTAAAAATGTTAGTATACCATCTTCTGAAGCTTTATCTTCGGATCCTTCC
TACAACAAAGAAAAACACATTATTCATCCTACCCAAAAGTCTAAAGCATCACAAGGTAGTGACCTTGAAC
AAAATGAAGCCTCAAGAAAGAATAAGAAAAAGAAAGAAAAATCTACATCAAAATATGAAGTCCTGACAGT
TCAAGAGCCTCCAAGGATTGAAGATGCCGAGGAATTTCCCAACCTGGCAGTTGCATCTGAAAGAAGAGAC
AGAATAGAGACACCGAAATTTCAATCTAAGCAGCAGCCACAGGATAATTTTAAAAATAATGTAAAGAAGA
GCCAGCTTCCAGTGCAGTTGGACTTGGGGGGCATGCTGACAGCCCTGGAGAAGAAGCAGCACTCTCAGCA
TGCAAAGCAGTCCTCCAAACCAGTGGTAGTCTCAGTTGGAGCAGTGCCAGTCCTTTCCAAAGAATGTGCA
TCAGGGGAGAGAGGCCGCCGCATGAGTCAAATGAAGACCCCGCACAATCCCTTGGACTCCAGCGCCCCAC
TGATGAAGAAAGGGAAGCAGAGGGAGATCCCCAAGGCCAAGAAGCCAACCTCACTGAAGAAGATTATTTT
GAAAGAACGGCAAGAGAGAAAGCAGCGTCTCCAAGAAAATGCTGTGGGTCCAGCTTTTACCAGTGATGAC
ACACAAGATGGAGAGAGTGGTGGTGATGACCAGTTTCCCGAGCAGGCAGAGCTGTCAGGGCCAGAGGGGA
TGGACGAACTGATCTCCACTCCTTCGGTTGAGGACAAGTCTGAAGAGCCACCAGGCACAGAGCTCCAGAG
GGACACAGAGGCCTCCCACCTTGCTCCCAATCACACCACCTTCCCTAAGATCCACAGCCGCAGATTCAGG
GATTACTGCAGCCAGATGCTTAGTAAAGAAGTGGATGCTTGTGTTACCGACCTACTCAAAGAACTGGTCC
GTTTCCAAGACCGTATGTACCAGAAAGATCCAGTCAAGGCCAAGACTAAACGTCGACTTGTGTTGGGGTT
GAGGGAGGTTCTCAAACACCTGAAGCTCAAAAAACTGAAATGTGTCATTATTTCTCCCAACTGTGAGAAG
ATACAGTCAAAAGGTGGGCTGGATGACACTTTGCACACAATTATTGATTATGCCTGTGAGCAGAACATTC
CCTTTGTGTTTGCTCTCAACCGCAAAGCTCTGGGGCGCAGTTTGAATAAGGCAGTTCCTGTCAGTGTGGT
GGGGATCTTCAGCTATGATGGGGCCCAGGATCAGTTCCACAAGATGGTTGAGCTGACAGTGGCGGCCCGA
CAGGCGTACAAGACCATGCTGGAGAATGTGCAGCAGGAGCTGGTGGGAGAGCCCAGGCCTCAGGCACCTC
CCAGCCTACCCACACAGGGCCCCAGCTGCCCTGCAGAAGATGGCCCCCCAGCCCTGAAAGAAAAAGAAGA
GCCACACTACATTGAAATCTGGAAAAAACATCTGGAAGCATACAGTGGATGTACCCTGGAGCTAGAAGAA
TCCTTGGAGGCTTCAACCTCTCAAATGATGAATTTGAATTTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206346 protein sequence
Red=Cloning site Green=Tags(s)

MASEGPREPESEGIKLSADVKPFVPRFAGLNVAWLESSEACVFPSSAATYYPFVQEPPVTEQKIYTEDMA
FGASTFPPQYLSSEITLHPYAYSPYTLDSTQNVYSVPGSQYLYNQPSCYRGFQTVKHRNENTCPLPQEMK
ALFKKKTYDEKKTYDQQKFDSERADGTISSEIKSARGSHHLSIYAENSLKSDGYHKRTDRKSRIIAKNVS
TSKPEFEFTTLDFPELQGAENNMSEIQKQPKWGPVHSVSTDISLLREVVKPAAVLSKGEIVVKNNPNESV
TANAATNSPSCTRELSWTPMGYVVRQTLSTELSAAPKNVTSMINLKTIASSADPKNVSIPSSEALSSDPS
YNKEKHIIHPTQKSKASQGSDLEQNEASRKNKKKKEKSTSKYEVLTVQEPPRIEDAEEFPNLAVASERRD
RIETPKFQSKQQPQDNFKNNVKKSQLPVQLDLGGMLTALEKKQHSQHAKQSSKPVVVSVGAVPVLSKECA
SGERGRRMSQMKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERKQRLQENAVGPAFTSDD
TQDGESGGDDQFPEQAELSGPEGMDELISTPSVEDKSEEPPGTELQRDTEASHLAPNHTTFPKIHSRRFR
DYCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLKKLKCVIISPNCEK
IQSKGGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQDQFHKMVELTVAAR
QAYKTMLENVQQELVGEPRPQAPPSLPTQGPSCPAEDGPPALKEKEEPHYIEIWKKHLEAYSGCTLELEE
SLEASTSQMMNLNL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024077
ORF Size 2562 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024077.5
RefSeq Size 3535 bp
RefSeq ORF 2565 bp
Locus ID 79048
UniProt ID Q96T21
Cytogenetics 9q22.2
Domains Ribosomal_L7Ae
MW 95.4 kDa
Summary The protein encoded by this gene is one of the essential components of the machinery involved in co-translational insertion of selenocysteine (Sec) into selenoproteins. Sec is encoded by the UGA codon, which normally signals translation termination. The recoding of UGA as Sec codon requires a Sec insertion sequence (SECIS) element; present in the 3' untranslated regions of eukaryotic selenoprotein mRNAs. This protein specifically binds to the SECIS element, which is stimulated by a Sec-specific translation elongation factor. Mutations in this gene have been associated with reduction in enzymatic activity of type II iodothyronine deiodinase (a selenoprotein) and abnormal thyroid hormone metabolism. Alternatively spliced transcript variants have been found for this gene. provided by RefSeq, Aug 2017
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"NM_024077" in other vectors (6)
SKU Description Size Price
RC206346L1 Lenti ORF clone of Human SECIS binding protein 2 (SECISBP2), Myc-DDK-tagged 10 ug
$1,082.00
RC206346L2 Lenti ORF clone of Human SECIS binding protein 2 (SECISBP2), mGFP tagged 10 ug
$1,082.00
RC206346L3 Lenti ORF clone of Human SECIS binding protein 2 (SECISBP2), Myc-DDK-tagged 10 ug
$1,082.00
RC206346L4 Lenti ORF clone of Human SECIS binding protein 2 (SECISBP2), mGFP tagged 10 ug
$1,082.00
RG206346 SECISBP2 (tGFP-tagged) - Human SECIS binding protein 2 (SECISBP2) 10 ug
$789.00 $982.00
SC112316 SECISBP2 (untagged)-Human SECIS binding protein 2 (SECISBP2) 10 ug
$783.00

Plasmid Map for RC206346

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206346
Circular map for RC206346

file-alt Citations

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