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TC2N (NM_152332) Human Tagged ORF Clone

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SKU
RC206331
TC2N (Myc-DDK-tagged)-Human tandem C2 domains, nuclear (TC2N), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TC2N
Synonyms C2CD1; C14orf47; MTAC2D1; Tac2-N
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206331 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAACAGAATTTATAAAGAGTTGCTGTGGAGGATGTTTCTATGGTGAAACAGAAAAACACAACTTTT
CTGTGGAAAGAGATTTTAAAGCAGCAGTCCCAAATAGTCAAAATGCTACTATCTCTGTACCTCCATTGAC
TTCTGTTTCTGTAAAGCCTCAGCTTGGCTGTACTGAGGATTATTTGCTTTCCAAATTACCATCTGATGGC
AAAGAAGTACCATTTGTGGTGCCCAAGTTTAAGTTATCTTACATTCAACCCAGGACACAAGAAACTCCTT
CACATCTGGAAGAACTTGAAGGATCTGCCAGAGCATCTTTTGGAGATCGAAAGGTAGAACTTTCCAGTTC
ATCCCAGCACGGACCTAGCTATGATGTGTATAACCCATTCTATATGTATCAGCACATTTCACCTGATTTG
AGTCGACGCTTTCCTCCCCGTTCAGAAGTGACGAGACTGTATGGATCGGTTTGTGATTTAAGGACGAACA
AACTTCCCGGTTCCCCTGGGCTAAGCAAATCTATGTTTGATCTTACAAACTCATCTCAGCGATTCATCCA
GAGACATGATTCATTGTCCAGTGTACCCAGTAGTTCTTCTTCAAGGAAAAATTCTCAGGGGAGTAACAGA
AGCCTGGATACAATTACTCTATCAGGAGATGAAAGGGACTTTGGGAGACTGAATGTGAAATTGTTTTATA
ATTCTTCAGTAGAACAGATCTGGATCACAGTTTTACAGTGCAGAGATTTAAGTTGGCCCTCTAGTTATGG
AGACACTCCTACTGTTTCTATAAAAGGAATACTTACATTGCCCAAACCAGTGCATTTCAAATCTTCAGCC
AAGGAAGGTTCCAACGCTATTGAATTTATGGAAACGTTTGTATTTGCTATTAAACTTCAAAATCTACAAA
CTGTAAGACTTGTATTTAAGATTCAAACCCAGACTCCCAGGAAGAAAACCATTGGAGAATGCTCAATGTC
ACTCAGAACCCTTAGCACACAGGAAATGGATTACTCTTTGGATATAACACCACCTTCAAAAATTTCTGTT
TGCCATGCAGAACTTGAATTGGGGACTTGTTTTCAAGCAGTAAATAGCAGAATTCAGTTACAAATTCTTG
AGGCACGGTACCTTCCAAGCTCATCAACACCTCTGACTTTGAGTTTTTTCGTGAAGGTGGGAATGTTTAG
CTCGGGAGAGTTGATTTATAAGAAAAAGACACGCTTACTGAAGGCCTCCAATGGAAGAGTCAAGTGGGGA
GAGACTATGATTTTTCCACTTATACAGAGTGAAAAAGAAATTGTTTTTCTCATTAAGCTTTACAGTCGAA
GCTCTGTAAGAAGAAAACACTTTGTGGGCCAGATTTGGATAAGTGAAGACAGTAATAACATTGAAGCAGT
GAACCAGTGGAAAGAGACAGTAATAAATCCAGAAAAGGTTGTTATCAGGTGGCACAAATTAAATCCATCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206331 protein sequence
Red=Cloning site Green=Tags(s)

MATEFIKSCCGGCFYGETEKHNFSVERDFKAAVPNSQNATISVPPLTSVSVKPQLGCTEDYLLSKLPSDG
KEVPFVVPKFKLSYIQPRTQETPSHLEELEGSARASFGDRKVELSSSSQHGPSYDVYNPFYMYQHISPDL
SRRFPPRSEVTRLYGSVCDLRTNKLPGSPGLSKSMFDLTNSSQRFIQRHDSLSSVPSSSSSRKNSQGSNR
SLDTITLSGDERDFGRLNVKLFYNSSVEQIWITVLQCRDLSWPSSYGDTPTVSIKGILTLPKPVHFKSSA
KEGSNAIEFMETFVFAIKLQNLQTVRLVFKIQTQTPRKKTIGECSMSLRTLSTQEMDYSLDITPPSKISV
CHAELELGTCFQAVNSRIQLQILEARYLPSSSTPLTLSFFVKVGMFSSGELIYKKKTRLLKASNGRVKWG
ETMIFPLIQSEKEIVFLIKLYSRSSVRRKHFVGQIWISEDSNNIEAVNQWKETVINPEKVVIRWHKLNPS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152332
ORF Size 1470 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152332.5
RefSeq Size 5222 bp
RefSeq ORF 1473 bp
Locus ID 123036
UniProt ID Q8N9U0
Cytogenetics 14q32.12
Domains C2
MW 55.3 kDa
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Documents
Resources
"NM_152332" in other vectors (6)
SKU Description Size Price
RC206331L1 Lenti ORF clone of Human tandem C2 domains, nuclear (TC2N), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC206331L2 Lenti ORF clone of Human tandem C2 domains, nuclear (TC2N), transcript variant 1, mGFP tagged 10 ug
$757.00
RC206331L3 Lenti ORF clone of Human tandem C2 domains, nuclear (TC2N), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC206331L4 Lenti ORF clone of Human tandem C2 domains, nuclear (TC2N), transcript variant 1, mGFP tagged 10 ug
$757.00
RG206331 TC2N (tGFP-tagged) - Human tandem C2 domains, nuclear (TC2N), transcript variant 1 10 ug
$489.00 $657.00
SC108327 TC2N (untagged)-Human tandem C2 domains, nuclear (TC2N), transcript variant 1 10 ug
$457.00

Plasmid Map for RC206331

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206331
Circular map for RC206331

file-alt Citations

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