KLHDC8A (NM_018203) Human Tagged ORF Clone

  • Product Brand Image
SKU
RC206258
KLHDC8A (Myc-DDK-tagged)-Human kelch domain containing 8A (KLHDC8A)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KLHDC8A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206258 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTGCCTAACGTCAAGGACTTCCAGTGGAAGCGCCTGGCGCCACTGCCCAGCCGCCGGGTCTACT
GCTCCCTGCTGGAGACCGGGGGCCAGGTCTATGCCATCGGGGGATGTGACGACAACGGCGTCCCCATGGA
CTGCTTCGAGGTCTACTCCCCGGAGGCCGACCAGTGGACCGCCTTGCCCCGGCTGCCCACAGCACGGGCG
GGGGTGGCCGTCACCGCCCTGGGGAAGCGGATCATGGTGATTGGGGGCGTGGGCACCAATCAGCTGCCCC
TGAAGGTCGTGGAGATGTACAACATCGATGAGGGCAAGTGGAAGAAGAGGAGCATGCTGCGTGAGGCCGC
CATGGGCATTTCTGTCACGGCCAAAGATTACCGAGTATATGCGGCAGGCGGGATGGGCCTGGACCTACGT
CCACACAACCACCTCCAACACTATGACATGCTGAAGGACATGTGGGTGTCCCTAGCACACATGCCCACCC
CGAGATATGCTGCCACCTCCTTCCTCCGAGGCTCCAAGATCTACGTGCTGGGGGGACGACAGTCCAAGTA
CGCGGTCAACGCTTTCGAGGTCTTTGACATCGAGACTCGCTCCTGGACCAAGTTTCCCAACATTCCCTAT
AAGCGGGCCTTCTCCAGCTTTGTGACCCTGGACAACCACTTGTACAGCCTAGGAGGCCTGCGGCAAGGTC
GCCTCTACCGGCAGCCAAAGTTCCTGCGGACGATGGACGTGTTCGACATGGAACAGGGGGGATGGCTGAA
GATGGAACGATCGTTCTTCCTCAAGAAGCGGCGGGCAGATTTTGTGGCTGGCTCTCTGAGTGGACGGGTC
ATAGTGGCTGGGGGACTTGGGAATCAACCCACTGTCCTGGAGACGGCGGAAGCATTCCACCCAGGGAAGA
ACAAATGGGAGATCCTCCCTGCCATGCCCACACCCCGCTGTGCCTGCTCCAGCATAGTCGTCAAGAACTG
CCTCCTCGCCGTGGGAGGTGTCAACCAGGGTCTGAGTGACGCAGTGGAGGCCCTGTGTGTCTCTGACTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC206258 protein sequence
Red=Cloning site Green=Tags(s)

MEVPNVKDFQWKRLAPLPSRRVYCSLLETGGQVYAIGGCDDNGVPMDCFEVYSPEADQWTALPRLPTARA
GVAVTALGKRIMVIGGVGTNQLPLKVVEMYNIDEGKWKKRSMLREAAMGISVTAKDYRVYAAGGMGLDLR
PHNHLQHYDMLKDMWVSLAHMPTPRYAATSFLRGSKIYVLGGRQSKYAVNAFEVFDIETRSWTKFPNIPY
KRAFSSFVTLDNHLYSLGGLRQGRLYRQPKFLRTMDVFDMEQGGWLKMERSFFLKKRRADFVAGSLSGRV
IVAGGLGNQPTVLETAEAFHPGKNKWEILPAMPTPRCACSSIVVKNCLLAVGGVNQGLSDAVEALCVSDS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018203
ORF Size 1050 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018203.3
RefSeq Size 3044 bp
RefSeq ORF 1053 bp
Locus ID 55220
UniProt ID Q8IYD2, A0A024R981
Cytogenetics 1q32.1
Domains Kelch
MW 38.9 kDa
Summary This gene encodes a kelch domain-containing protein which is upregulated in cancer. Upregulated expression of the encoded protein may provide an alternative pathway for tumors to maintain aggressiveness in the absence of epidermal growth factor receptor dependence. Alternative splicing results in multiple transcript variants. provided by RefSeq, Dec 2012
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_018203" in other vectors (4)
SKU Description Size Price
RC206258L3 Lenti ORF clone of Human kelch domain containing 8A (KLHDC8A), Myc-DDK-tagged 10 ug
$757.00
RC206258L4 Lenti ORF clone of Human kelch domain containing 8A (KLHDC8A), mGFP tagged 10 ug
$757.00
RG206258 KLHDC8A (tGFP-tagged) - Human kelch domain containing 8A (KLHDC8A) 10 ug
$489.00 $657.00
SC113641 KLHDC8A (untagged)-Human kelch domain containing 8A (KLHDC8A) 10 ug
$457.00

Plasmid Map for RC206258

Download sequence for

Cloning scheme for RC206258

Circular map for RC206258

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products