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TEM7 (PLXDC1) (NM_020405) Human Tagged ORF Clone

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SKU
RC206233
PLXDC1 (Myc-DDK-tagged)-Human plexin domain containing 1 (PLXDC1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TEM7
Synonyms TEM3; TEM7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206233 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGAGGCGAGCTCTGGCTCCTGGTGCTGGTGCTCAGGGAGGCTGCCCGGGCGCTGAGCCCCCAGCCCG
GAGCAGGTCACGATGAGGGCCCAGGCTCTGGATGGGCTGCCAAAGGGACCGTGCGGGGCTGGAACCGGAG
AGCCCGAGAGAGCCCTGGGCATGTGTCAGAGCCGGACAGGACCCAGCTGAGCCAGGACCTGGGTGGGGGC
ACCCTGGCCATGGACACGCTGCCAGATAACAGGACCAGGGTGGTGGAGGACAACCACAGCTATTATGTGT
CCCGTCTCTATGGCCCCAGCGAGCCCCACAGCCGGGAACTGTGGGTAGATGTGGCCGAGGCCAACCGGAG
CCAAGTGAAGATCCACACAATACTCTCCAACACCCACCGGCAGGCTTCGAGAGTGGTCTTGTCCTTTGAT
TTCCCTTTCTACGGGCATCCTCTGCGGCAGATCACCATGGCAACTGGAGGCTTCATCTTCATGGGGGACG
TGATCCATCGGATGCTCACAGCTACTCAGTATGTGGCGCCCCTGATGGCCAACTTCAACCCTGGCTACTC
CGACAACTCCACAGTTGTTTACTTTGACAATGGGACAGTCTTTGTGGTTCAGTGGGACCACGTTTATCTC
CAAGGCTGGGAAGACAAGGGCAGTTTCACCTTCCAGGCAGCTCTGCACCATGACGGCCGCATTGTCTTTG
CCTATAAAGAGATCCCTATGTCTGTCCCGGAAATCAGCTCCTCCCAGCATCCTGTCAAAACCGGCCTATC
GGATGCCTTCATGATTCTCAATCCATCCCCGGATGTGCCAGAATCTCGGCGAAGGAGCATCTTTGAATAT
CACCGCATAGAGCTGGACCCCAGCAAGGTCACCAGCATGTCGGCCGTGGAGTTCACCCCATTGCCGACCT
GCCTGCAGCATAGGAGCTGTGACGCCTGCATGTCCTCAGACCTGACCTTCAACTGCAGCTGGTGCCATGT
CCTCCAGAGATGCTCCAGTGGCTTTGACCGCTATCGCCAGGAGTGGATGGACTATGGCTGTGCACAGGAG
GCAGAGGGCAGGATGTGCGAGGACTTCCAGGATGAGGACCACGACTCAGCCTCCCCTGACACTTCCTTCA
GCCCCTATGATGGAGACCTCACCACTACCTCCTCCTCCCTCTTCATCGACAGCCTCACCACAGAAGATGA
CACCAAGTTGAATCCCTATGCAGGAGGAGACGGCCTTCAGAACAACCTGTCACCCAAGACAAAGGGCACT
CCTGTGCACCTGGGCACCATCGTGGGCATCGTGCTGGCAGTCCTCCTCGTGGCGGCCATCATCCTGGCTG
GAATTTACATCAATGGCCACCCCACATCCAATGCTGCGCTCTTCTTCATCGAGCGTAGACCTCACCACTG
GCCAGCCATGAAGTTTCGCAGCCACCCTGACCATTCCACCTATGCGGAGGTGGAGCCCTCGGGCCATGAG
AAGGAGGGCTTCATGGAGGCTGAGCAGTGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206233 protein sequence
Red=Cloning site Green=Tags(s)

MRGELWLLVLVLREAARALSPQPGAGHDEGPGSGWAAKGTVRGWNRRARESPGHVSEPDRTQLSQDLGGG
TLAMDTLPDNRTRVVEDNHSYYVSRLYGPSEPHSRELWVDVAEANRSQVKIHTILSNTHRQASRVVLSFD
FPFYGHPLRQITMATGGFIFMGDVIHRMLTATQYVAPLMANFNPGYSDNSTVVYFDNGTVFVVQWDHVYL
QGWEDKGSFTFQAALHHDGRIVFAYKEIPMSVPEISSSQHPVKTGLSDAFMILNPSPDVPESRRRSIFEY
HRIELDPSKVTSMSAVEFTPLPTCLQHRSCDACMSSDLTFNCSWCHVLQRCSSGFDRYRQEWMDYGCAQE
AEGRMCEDFQDEDHDSASPDTSFSPYDGDLTTTSSSLFIDSLTTEDDTKLNPYAGGDGLQNNLSPKTKGT
PVHLGTIVGIVLAVLLVAAIILAGIYINGHPTSNAALFFIERRPHHWPAMKFRSHPDHSTYAEVEPSGHE
KEGFMEAEQC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020405
ORF Size 1500 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020405.5
RefSeq Size 6271 bp
RefSeq ORF 1503 bp
Locus ID 57125
UniProt ID Q8IUK5
Cytogenetics 17q12
Domains PSI
Protein Families Druggable Genome, Secreted Protein, Transmembrane
MW 55.8 kDa
Summary Plays a critical role in endothelial cell capillary morphogenesis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_020405" in other vectors (6)
SKU Description Size Price
RC206233L1 Lenti ORF clone of Human plexin domain containing 1 (PLXDC1), Myc-DDK-tagged 10 ug
$757.00
RC206233L2 Lenti ORF clone of Human plexin domain containing 1 (PLXDC1), mGFP tagged 10 ug
$757.00
RC206233L3 Lenti ORF clone of Human plexin domain containing 1 (PLXDC1), Myc-DDK-tagged 10 ug
$757.00
RC206233L4 Lenti ORF clone of Human plexin domain containing 1 (PLXDC1), mGFP tagged 10 ug
$757.00
RG206233 PLXDC1 (tGFP-tagged) - Human plexin domain containing 1 (PLXDC1) 10 ug
$489.00 $657.00
SC113127 PLXDC1 (untagged)-Human plexin domain containing 1 (PLXDC1) 10 ug
$466.00

Plasmid Map for RC206233

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206233
Circular map for RC206233

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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