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C2CD6 (NM_152525) Human Tagged ORF Clone

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SKU
RC206143
ALS2CR11 (Myc-DDK-tagged)-Human amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 11 (ALS2CR11), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $580.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol C2CD6
Synonyms ALS2CR11
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206143 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCACCCCAAGAGACAAATAGGCCTTTCAGCACACTTGATAACCGCAGCGGGCAGGTCCAAGTCC
TGTCCGCCACCCCGCTATTGCAGAGGAATCCCTACAGCAGCCCGGATATCATGCACATTAAAGGGTCGGA
GGCTTCTTCGGTCCCTTACGCCCTGAACCAGGGCACGACGGCCCTGCCTAAGAACAAGAACCAGGAGGGC
ACCGGGCACCGGCTGCTGAACATGCTGAGAAAAACTCTTAAAGAGTCTGATAGTGAAGAACTGGAAATAA
CACAGGAGACACCAAATTTGGTGCCATTTGGGGATGTGGTTGGCTGCCTGGGCATTCATATAAAGAACTG
CAGACATTTTATGCCTAAGATCAGTTTACAACATTATGCTAATTTATTCATTCGCATCTCTATAAACAAA
GCTGTGAAATGTACAAAAATGTGTAGCTTGCTATCCAAAAACGATGAGAAGAACACTGTAATTAAGTTCG
ATGAAGTGAAGTATTTTTCTGTACAGGTTCCCAGACGTTATGATGATAAGCGGAATAATATTTTATTGGA
ACTCATACAATATGACAATAGAGAAAAACGTGCTTTCTTGTTAGGGAGTGTTCAGATACATCTTTATGAG
GTAATTCAGAAAGGGTGCTTCATTGAAGAGGTCCAAGTGTTGCATGGAAACATATTTGTCTGCAGGCTGG
AGGTGGAATTTATGTTCTCCTATGGAAACTTTGGTTATGGATTTTCACATCAGTTAAAACCTCTTCAGAA
AATTACTGAGCCATCCATGTTTATGAATCTTGCACCACCTCCAGAAAGAACAGATCCCGTGACAAAAGTT
ATTACACCACAGACAGTAGAATATCCAGCATTCTTATCCCCAGACCTGAATGTTACTGTTGGGACTCCAG
CTGTGCAATCCTCCAACCAGCCATCTGTAGTGCGACTTGAAAAACTTCAGCAACAACCCCGGGAAAGGCT
GGAAAAGATGAAAAAAGAATATAGAAATCTGAATACATGGATAGATAAAGCTAACTATTTAGAAAGCATT
CTAATGCCAAAATTGGAACATAAGGATTCTGAGGAAACTAATATAGATGAGGCATCTGAAAACACGAAAA
GTAATCAACCTGAAGAGGAGCTTGAAAATATTGTAGGAGTAGATATCCCTCTTGTAAATGAGGAAGCTGA
AACTACTGCAAATGAGTTACTGGATAATGATTCTGAGAAAGGCCTGACTATACCAACTTTGAACCAATCG
GACCAAGATAATTCAACTGCTGATGCTTCTAAAAATGATGAATCAACACCTTCACCCACAGAAGTGCACT
CATTGTGCACTATTTCTAACCAGGAAACAATAAAAGCAGGCAGAATACCACCTTTAGGTGAACGTCAATC
AGAAAGCATGCCAGACAGAAAAATGAAAAATGTATTTTTTCCATTAGAAGTAAAATTAAAAGATAACTAT
CCAAGCATTTTAAAAGCAGACTCATCTCTATCAGAGGTGGCTTTTTCACCAAAAGAATATAATTCACCAT
CTTTCAGACCAGAATATATAGAATTCAAACCAAAATTTCAGGATTGTTCAGATAAATTTGAGGATTTACA
TGACATGACCTCATTTACACACCTTAAAAAAGTAAAATCAAGATCAAGGCTTTTAGGAAAAAGCTCAGAT
GATATCCATAACCATGCCAGACACTCTGCAAGACCATATACTGCTCCGGAGGTTAACAAGCAACGAGAAA
GCTACAGTGGAAAGTTTACAAGTCGTCGAATGGTTTCATCAGGCTTAGTTCATATAAATGATAAAACATC
AGATTATGAAATGCATAAAATGCGGCCAAAAAAAATTAAAAGAGGATAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206143 protein sequence
Red=Cloning site Green=Tags(s)

MEPPQETNRPFSTLDNRSGQVQVLSATPLLQRNPYSSPDIMHIKGSEASSVPYALNQGTTALPKNKNQEG
TGHRLLNMLRKTLKESDSEELEITQETPNLVPFGDVVGCLGIHIKNCRHFMPKISLQHYANLFIRISINK
AVKCTKMCSLLSKNDEKNTVIKFDEVKYFSVQVPRRYDDKRNNILLELIQYDNREKRAFLLGSVQIHLYE
VIQKGCFIEEVQVLHGNIFVCRLEVEFMFSYGNFGYGFSHQLKPLQKITEPSMFMNLAPPPERTDPVTKV
ITPQTVEYPAFLSPDLNVTVGTPAVQSSNQPSVVRLEKLQQQPRERLEKMKKEYRNLNTWIDKANYLESI
LMPKLEHKDSEETNIDEASENTKSNQPEEELENIVGVDIPLVNEEAETTANELLDNDSEKGLTIPTLNQS
DQDNSTADASKNDESTPSPTEVHSLCTISNQETIKAGRIPPLGERQSESMPDRKMKNVFFPLEVKLKDNY
PSILKADSSLSEVAFSPKEYNSPSFRPEYIEFKPKFQDCSDKFEDLHDMTSFTHLKKVKSRSRLLGKSSD
DIHNHARHSARPYTAPEVNKQRESYSGKFTSRRMVSSGLVHINDKTSDYEMHKMRPKKIKRGY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152525
ORF Size 1869 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152525.3, NP_689738.2
RefSeq Size 2123 bp
RefSeq ORF 1872 bp
Locus ID 151254
UniProt ID Q53TS8, A0A140VJE2
Cytogenetics 2q33.1
MW 71.1 kDa
Summary An autosomal recessive form of juvenile amyotrophic lateral sclerosis was originally mapped to a region of chromosome 2 that includes this gene. The encoded protein contains a calcium-dependent membrane targeting C2 domain. This domain is often found in proteins that are involved in membrane trafficking and signal transduction. provided by RefSeq, Jun 2016
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Documents
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"NM_152525" in other vectors (4)
SKU Description Size Price
RC206143L3 Lenti ORF clone of Human amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 11 (ALS2CR11), transcript variant 2, Myc-DDK-tagged 10 ug
$880.00
RC206143L4 Lenti ORF clone of Human amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 11 (ALS2CR11), transcript variant 2, mGFP tagged 10 ug
$880.00
RG206143 ALS2CR11 (tGFP-tagged) - Human amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 11 (ALS2CR11), transcript variant 2 10 ug
$489.00 $780.00
SC123257 ALS2CR11 (untagged)-Human amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 11 (ALS2CR11), transcript variant 2 10 ug
$581.00

Plasmid Map for RC206143

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206143
Circular map for RC206143

file-alt Citations

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