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MPP7 (NM_173496) Human Tagged ORF Clone

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SKU
RC206096
MPP7 (Myc-DDK-tagged)-Human membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7) (MPP7)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $536.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MPP7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC206096 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAGCTTTGTCAACGGGATCTGGGAGTGACACTGGTCTGTATGAGCTGTTGGCTGCTCTGCCAGCCC
AGCTGCAGCCACATGTGGATAGCCAGGAAGACCTGACCTTCCTCTGGGATATGTTTGGTGAAAAAAGCCT
GCATTCATTGGTAAAGATTCATGAAAAACTACACTACTATGAGAAGCAGAGTCCGGTGCCCATTCTCCAT
GGTGCGGCGGCCTTGGCCGATGATCTGGCCGAAGAGCTTCAGAACAAGCCATTAAACAGTGAGATCAGAG
AGCTGTTGAAACTACTGTCAAAACCCAATGTGAAGGCTTTGCTCTCTGTACATGATACTGTGGCTCAGAA
GAATTACGACCCAGTGTTGCCTCCTATGCCTGAAGATATTGACGATGAGGAAGACTCAGTAAAAATAATC
CGTCTGGTCAAAAATAGAGAACCACTGGGAGCTACCATTAAGAAGGATGAACAGACCGGGGCGATCATTG
TGGCCAGAATCATGAGAGGAGGAGCTGCAGATAGAAGTGGTCTTATTCATGTTGGTGATGAACTTAGGGA
AGTCAACGGGATACCAGTGGAGGATAAAAGGCCTGAGGAAATAATACAGATTTTGGCTCAGTCTCAGGGA
GCAATTACATTTAAGATTATACCCGGCAGCAAAGAGGAGACACCATCAAAAGAAGGCAAGATGTTTATCA
AAGCCCTCTTTGACTATAATCCTAATGAGGATAAGGCAATTCCATGTAAGGAAGCTGGGCTTTCTTTCAA
AAAGGGAGATATTCTTCAGATTATGAGCCAAGATGATGCAACTTGGTGGCAAGCGAAACACGAAGCTGAT
GCCAACCCCAGGGCAGGCTTGATCCCCTCAAAGCATTTCCAGGAAAGGAGATTGGCTTTGAGACGACCAG
AAATATTGGTTCAGCCCCTGAAAGTTTCCAACAGGAAATCATCTGGTTTTAGAAGAAGTTTTCGTCTTAG
TAGAAAAGATAAGAAAACAAATAAATCCATGTATGAATGCAAGAAGAGTGATCAGTACGACACAGCTGAC
GTACCCACATACGAAGAAGTGACACCGTATCGGCGACAAACTAATGAAAAATACAGACTCGTTGTCTTGG
TTGGTCCCGTGGGAGTAGGGCTGAATGAACTGAAACGAAAGCTGCTGATCAGTGACACACAGCACTATGG
CGTGACAGTGCCCCATACCACCAGAGCAAGAAGAAGCCAGGAGAGTGATGGTGTTGAATACATTTTCATT
TCCAAGCATTTGTTTGAGACAGATGTACAAAATAACAAGTTTATTGAATATGGAGAATATAAAAACAACT
ACTACGGCACAAGTATAGACTCAGTTCGGTCTGTCCTTGCTAAAAACAAAGTTTGTTTGTTGGATGTTCA
GCCTCATACAGTGAAGCATTTAAGGACACTAGAATTTAAGCCCTATGTGATATTTATAAAGCCTCCATCA
ATAGAGCGTTTGAGAGAAACAAGAAAAAATGCAAAGATTATTTCAAGCAGAGATGACCAAGGTGCTGCAA
AACCCTTCACAGAAGAAGATTTTCAAGAAATGATTAAATCTGCACAGATAATGGAAAGTCAATATGGTCA
TCTTTTTGACAAAATTATAATAAATGATGACCTCACTGTGGCATTTAATGAGCTCAAAACAACTTTTGAC
AAATTAGAGACAGAGACCCATTGGGTGCCAGTGAGCTGGTTACATTCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC206096 protein sequence
Red=Cloning site Green=Tags(s)

MPALSTGSGSDTGLYELLAALPAQLQPHVDSQEDLTFLWDMFGEKSLHSLVKIHEKLHYYEKQSPVPILH
GAAALADDLAEELQNKPLNSEIRELLKLLSKPNVKALLSVHDTVAQKNYDPVLPPMPEDIDDEEDSVKII
RLVKNREPLGATIKKDEQTGAIIVARIMRGGAADRSGLIHVGDELREVNGIPVEDKRPEEIIQILAQSQG
AITFKIIPGSKEETPSKEGKMFIKALFDYNPNEDKAIPCKEAGLSFKKGDILQIMSQDDATWWQAKHEAD
ANPRAGLIPSKHFQERRLALRRPEILVQPLKVSNRKSSGFRRSFRLSRKDKKTNKSMYECKKSDQYDTAD
VPTYEEVTPYRRQTNEKYRLVVLVGPVGVGLNELKRKLLISDTQHYGVTVPHTTRARRSQESDGVEYIFI
SKHLFETDVQNNKFIEYGEYKNNYYGTSIDSVRSVLAKNKVCLLDVQPHTVKHLRTLEFKPYVIFIKPPS
IERLRETRKNAKIISSRDDQGAAKPFTEEDFQEMIKSAQIMESQYGHLFDKIIINDDLTVAFNELKTTFD
KLETETHWVPVSWLHS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173496
ORF Size 1728 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173496.2, NP_775767.1
RefSeq Size 5207 bp
RefSeq ORF 1731 bp
Locus ID 143098
UniProt ID Q5T2T1
Cytogenetics 10p12.1
Protein Families Druggable Genome
MW 65.6 kDa
Summary The protein encoded by this gene is a member of the p55 Stardust family of membrane-associated guanylate kinase (MAGUK) proteins, which function in the establishment of epithelial cell polarity. This family member forms a complex with the polarity protein DLG1 (discs, large homolog 1) and facilitates epithelial cell polarity and tight junction formation. Polymorphisms in this gene are associated with variations in site-specific bone mineral density (BMD). Alternative splicing results in multiple transcript variants. provided by RefSeq, Dec 2015
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"NM_173496" in other vectors (4)
SKU Description Size Price
RC206096L3 Lenti ORF clone of Human membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7) (MPP7), Myc-DDK-tagged 10 ug
$836.00
RC206096L4 Lenti ORF clone of Human membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7) (MPP7), mGFP tagged 10 ug
$836.00
RG206096 MPP7 (tGFP-tagged) - Human membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7) (MPP7) 10 ug
$489.00 $736.00
SC101157 MPP7 (untagged)-Human membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7) (MPP7) 10 ug
$537.00

Plasmid Map for RC206096

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC206096
Circular map for RC206096

file-alt Citations

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