CBLB (NM_170662) Human Tagged ORF Clone
SKU
RC206047
CBLB (Myc-DDK-tagged)-Human Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB)
$1,348.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CBLB |
| Synonyms | Cbl-b; Nbla00127; RNF56 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_170662 |
| ORF Size | 2946 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_170662.2 |
| RefSeq Size | 3976 bp |
| RefSeq ORF | 2949 bp |
| Locus ID | 868 |
| UniProt ID | Q13191 |
| Cytogenetics | 3q13.11 |
| Protein Families | Druggable Genome |
| Protein Pathways | Chronic myeloid leukemia, Endocytosis, ErbB signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, Pathways in cancer, T cell receptor signaling pathway, Ubiquitin mediated proteolysis |
| MW | 109.4 kDa |
| Summary | This gene encodes an E3 ubiquitin-protein ligase which promotes proteosome-mediated protein degradation by transferring ubiquitin from an E2 ubiquitin-conjugating enzyme to a substrate. The encoded protein is involved in the regulation of immune response by limiting T-cell receptor, B-cell receptor, and high affinity immunoglobulin epsilon receptor activation. Studies in mouse suggest that this gene is involved in antifungal host defense and that its inhibition leads to increased fungal killing. Manipulation of this gene may be beneficial in implementing immunotherapies for a variety of conditions, including cancer, autoimmune diseases, allergies, and infections. provided by RefSeq, Sep 2017 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_170662" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC206047L1 | Lenti ORF clone of Human Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB), Myc-DDK-tagged | 10 ug |
$1,648.00
|
|
| RC206047L2 | Lenti ORF clone of Human Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB), mGFP tagged | 10 ug |
$1,648.00
|
|
| RC206047L3 | Lenti ORF clone of Human Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB), Myc-DDK-tagged | 10 ug |
$1,648.00
|
|
| RC206047L4 | Lenti ORF clone of Human Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB), mGFP tagged | 10 ug |
$1,648.00
|
|
| RG206047 | CBLB (tGFP-tagged) - Human Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB) | 10 ug |
$1,548.00
|
|
| SC107022 | CBLB (untagged)-Human Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB) | 10 ug |
$1,350.00
|
|
Citations
LncRNA DUXAP9-206 directly binds with Cbl-b to augment EGFR signaling and promotes non-small cell lung cancer progression
Zhu, An, Choy et al
J. Cell. Mol. Med. (2018)
Zhu, An, Choy et al
J. Cell. Mol. Med. (2018)
AI Summary: OriGene products pCMV6-Entry-EGFR and pCMV6-Entry-Cbl-b were used in this study to overexpress EGFR and Cbl-b, enabling the investigation of how lncRNA DUXAP9-206 modulates EGFR signaling and promotes non-small cell lung cancer progression.
Snippet: oligonucleotides were annealed and then ligated into the pSuper‐retro‐puro plasmid to generate pSuper‐DUXAP9‐206‐ RNAi#1 and pSuper‐DUXAP9‐206‐RNAi#2. pCMV6‐Entry‐EGFR and pCMV6‐Entry‐Cbl‐b were purchased from Origene Technologies
CBL mutations in myeloproliferative neoplasms are also found in the gene's proline-rich domain and in patients with the V617FJAK2
Aranaz, Hurtado, Erquiaga et al
Haematologica (2012) 97 (8), 1234-41 DOI: 10.3324/haematol.2011.052605
Aranaz, Hurtado, Erquiaga et al
Haematologica (2012) 97 (8), 1234-41 DOI: 10.3324/haematol.2011.052605
Cited by 23
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
