beta COP (COPB1) (NM_016451) Human Tagged ORF Clone
SKU
RC206009
COPB1 (Myc-DDK-tagged)-Human coatomer protein complex, subunit beta 1 (COPB1), transcript variant 1
$872.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | beta COP |
| Synonyms | BARMACS; COPB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_016451 |
| ORF Size | 2859 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_016451.4 |
| RefSeq Size | 3490 bp |
| RefSeq ORF | 2862 bp |
| Locus ID | 1315 |
| UniProt ID | P53618 |
| Cytogenetics | 11p15.2 |
| Domains | Adaptin_N |
| MW | 107.1 kDa |
| Summary | This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described. provided by RefSeq, Jan 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_016451" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC206009L1 | Lenti ORF clone of Human coatomer protein complex, subunit beta 1 (COPB1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,172.00
|
|
| RC206009L2 | Lenti ORF clone of Human coatomer protein complex, subunit beta 1 (COPB1), transcript variant 1, mGFP tagged | 10 ug |
$1,172.00
|
|
| RC206009L3 | Lenti ORF clone of Human coatomer protein complex, subunit beta 1 (COPB1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,172.00
|
|
| RC206009L4 | Lenti ORF clone of Human coatomer protein complex, subunit beta 1 (COPB1), transcript variant 1, mGFP tagged | 10 ug |
$1,172.00
|
|
| RG206009 | COPB1 (tGFP-tagged) - Human coatomer protein complex, subunit beta 1 (COPB1), transcript variant 1 | 10 ug |
$789.00
$1,072.00
|
|
| SC108296 | COPB1 (untagged)-Human coatomer protein complex, subunit beta 1 (COPB1), transcript variant 1 | 10 ug |
$873.00
|
|
Citations
Biallelic variants in COPB1 cause a novel, severe intellectual disability syndrome with cataracts and variable microcephaly
Macken, Godwin, Wheway et al
Genome Med (2021) 13 (1), 34 DOI: 10.1186/s13073-021-00850-w
Macken, Godwin, Wheway et al
Genome Med (2021) 13 (1), 34 DOI: 10.1186/s13073-021-00850-w
Cited by 37
AI Summary: OriGene's Myc-DDK-tagged COPB1 transcript 1 (NM_016451) expression vector was used to introduce and study patient-specific COPB1 mutations in cell lines, enabling functional analysis of the disease-associated variants described in the paper.
Snippet: fGAP2 uncoating factor (pdb file 5NZS). Site-directed mutagenesis of COPB1 constructs Specific patient mutation was introduced into a Myc-DDK-tagged COPB1 transcript 1 (NM_016451) expression vector (Origene, MD, USA) using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs, MA, USA) following manufacturer’s instructions with customised primers designed using NEB Base Changer [45] (COPB1_
Showing 1-1 of 1 papers.
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