MAP4K5 (NM_198794) Human Tagged ORF Clone
SKU
RC205952
MAP4K5 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 2
$775.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | MAP4K5 |
| Synonyms | GCKR; KHS; KHS1; MAPKKKK5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_198794 |
| ORF Size | 2538 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_198794.3 |
| RefSeq Size | 4435 bp |
| RefSeq ORF | 2541 bp |
| Locus ID | 11183 |
| UniProt ID | Q9Y4K4, B3KWC4 |
| Cytogenetics | 14q22.1 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 95.1 kDa |
| Summary | This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_198794" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205952L1 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,075.00
|
|
| RC205952L2 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 2, mGFP tagged | 10 ug |
$1,075.00
|
|
| RC205952L3 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,075.00
|
|
| RC205952L4 | Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 2, mGFP tagged | 10 ug |
$1,075.00
|
|
| RG205952 | MAP4K5 (tGFP-tagged) - Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 2 | 10 ug |
$789.00
$975.00
|
|
| SC127493 | MAP4K5 (untagged)-Human mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 2 | 10 ug |
$776.00
|
|
Citations
Inheritance of Rare Functional GCKR Variants and Their Contribution to Triglyceride Levels in Families
Rees, Raimondo, Wang et al
Hum. Mol. Genet. (2014)
Rees, Raimondo, Wang et al
Hum. Mol. Genet. (2014)
Cited by 28
AI Summary: OriGene products contributed to this study by providing the Myc-DDK-tagged GCKR in the pCMV6 mammalian expression vector and monoclonal antibodies, which were used for functional characterization of GCKR variants.
Snippet: described previously (9, 29). Myc-DDK-tagged GCKR in the pCMV6 mammalian expression vector was obtained from Origene (Cedarlane Laboratories). Mutagenesis ... monoclonal antibody (1:2000 dilution, both from Origene Technologies) overnight. Signals ... Cite SaveSaving
Showing 1-1 of 1 papers.
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