CLRN3 (NM_152311) Human Tagged ORF Clone

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SKU
RC205944
CLRN3 (Myc-DDK-tagged)-Human clarin 3 (CLRN3)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CLRN3
Synonyms TMEM12; USH3AL1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205944 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTACCACAAAGAAGACATTGATGTTCTTATCAAGCTTTTTCACCAGCCTTGGGTCCTTCATTGTAA
TTTGCTCTATTCTTGGGACACAAGCATGGATCACCAGTACGATTGCTGTTAGAGACTCTGCTTCAAATGG
GAGCATTTTCATCACTTACGGACTTTTTCGTGGGGAGAGTAGTGAAGAATTGAGTCACGGACTTGCAGAA
CCAAAGAAAAAGTTTGCAGTTTTAGAGATACTGAATAATTCTTCCCAAAAAACTCTGCATTCGGTGACTA
TCCTGTTCCTGGTCCTGAGTTTGATCACGTCGCTGCTGAGCTCTGGGTTTACCTTCTACAACAGCATCAG
CAACCCTTACCAGACATTCCTGGGGCCGACGGGGGTGTACACCTGGAACGGGCTCGGTGCATCCTTCGTT
TTTGTGACCATGATACTGTTTGTGGCGAACACGCAGTCCAACCAACTCTCCGAAGAGTTGTTCCAAATGC
TTTACCCGGCAACCACCAGTAAAGGAACGACCCACAGTTACGGATACTCGTTCTGGCTCATACTGCTCGT
CATTCTTCTAAATATAGTCACTGTAACCATCATCATTTTCTACCAGAAGGCCAGATACCAGCGGAAGCAG
GAGCAGAGAAAGCCAATGGAATATGCTCCAAGGGACGGAATTTTATTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC205944 protein sequence
Red=Cloning site Green=Tags(s)

MPTTKKTLMFLSSFFTSLGSFIVICSILGTQAWITSTIAVRDSASNGSIFITYGLFRGESSEELSHGLAE
PKKKFAVLEILNNSSQKTLHSVTILFLVLSLITSLLSSGFTFYNSISNPYQTFLGPTGVYTWNGLGASFV
FVTMILFVANTQSNQLSEELFQMLYPATTSKGTTHSYGYSFWLILLVILLNIVTVTIIIFYQKARYQRKQ
EQRKPMEYAPRDGILF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152311
ORF Size 678 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152311.2
RefSeq Size 1174 bp
RefSeq ORF 681 bp
Locus ID 119467
UniProt ID Q8NCR9
Cytogenetics 10q26.2
Protein Families Transmembrane
MW 25.3 kDa
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Documents
Resources
"NM_152311" in other vectors (4)
SKU Description Size Price
RC205944L3 Lenti ORF clone of Human clarin 3 (CLRN3), Myc-DDK-tagged 10 ug
$600.00
RC205944L4 Lenti ORF clone of Human clarin 3 (CLRN3), mGFP tagged 10 ug
$600.00
RG205944 CLRN3 (tGFP-tagged) - Human clarin 3 (CLRN3) 10 ug
$489.00 $500.00
SC306363 CLRN3 (untagged)-Human clarin 3 (CLRN3) 10 ug
$330.00

Plasmid Map for RC205944

Download sequence for

Cloning scheme for RC205944

Circular map for RC205944

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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