CCDC23 (SVBP) (NM_199342) Human Tagged ORF Clone

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SKU
RC205925
CCDC23 (Myc-DDK-tagged)-Human coiled-coil domain containing 23 (CCDC23)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CCDC23
Synonyms CCDC23; NEDAHM
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205925 representing NM_199342
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCCACCTGCACGTAAAGAAAAAACCAAAGTTAAAGAATCTGTCAGCAGAGTTGAGAAGGCCAAAC
AGAAATCAGCCCAGCAGGAGCTGAAGCAGAGACAAAGAGCAGAGATCTATGCCCTCAACAGAGTCATGAC
AGAACTGGAGCAGCAGCAGTTTGATGAGTTCTGTAAACAGATGCAGCCTCCTGGAGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC205925 representing NM_199342
Red=Cloning site Green=Tags(s)

MDPPARKEKTKVKESVSRVEKAKQKSAQQELKQRQRAEIYALNRVMTELEQQQFDEFCKQMQPPGE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_199342
ORF Size 198 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_199342.4
RefSeq Size 684 bp
RefSeq ORF 201 bp
Locus ID 374969
UniProt ID Q8N300
Cytogenetics 1p34.2
MW 7.6 kDa
Summary Enhances the tyrosine carboxypeptidase activity of VASH1 and VASH2, thereby promoting the removal of the C-terminal tyrosine residue of alpha-tubulin (PubMed:29146869). Also required to enhance the solubility and secretion of VASH1 and VASH2 (PubMed:20736312, PubMed:27879017).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_199342" in other vectors (6)
SKU Description Size Price
RC205925L1 Lenti ORF clone of Human coiled-coil domain containing 23 (CCDC23), Myc-DDK-tagged 10 ug
$450.00
RC205925L2 Lenti ORF clone of Human coiled-coil domain containing 23 (CCDC23), mGFP tagged 10 ug
$450.00
RC205925L3 Lenti ORF clone of Human coiled-coil domain containing 23 (CCDC23), Myc-DDK-tagged 10 ug
$450.00
RC205925L4 Lenti ORF clone of Human coiled-coil domain containing 23 (CCDC23), mGFP tagged 10 ug
$450.00
RG205925 CCDC23 (tGFP-tagged) - Human coiled-coil domain containing 23 (CCDC23) 10 ug
$489.00
SC121611 CCDC23 (untagged)-Human coiled-coil domain containing 23 (CCDC23) 10 ug
$150.00

Plasmid Map for RC205925

Download sequence for

Cloning scheme for RC205925

Circular map for RC205925

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