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STAC (NM_003149) Human Tagged ORF Clone

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SKU
RC205851
STAC (Myc-DDK-tagged)-Human SH3 and cysteine rich domain (STAC)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol STAC
Synonyms STAC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205851 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCCCTCCGAGCAGCCCCCGCGAGGACGGCGTGGACGGGCTGCCCAAGGAGGCGGTGGGCGCCGAGC
AACCGCCCTCTCCTGCATCCACCAGCAGCCAGGAATCCAAGCTCCAGAAACTAAAACGATCACTTTCTTT
CAAGACCAAGAGTTTACGGAGCAAAAGTGCTGACAACTTCTTCCAGCGAACCAACAGCGAAGACATGAAA
CTGCAAGCACACATGGTGGCTGAGATCAGCCCCAGCTCCAGCCCACTCCCTGCTCCAGGAAGCCTGACGT
CCACACCCGCCAGGGCTGGTCTGCATCCAGGTGGCAAGGCTCATGCCTTTCATGAATACATCTTCAAGAA
GCCCACTTTCTGTGATGTCTGCAACCACATGATAGTGGGAACAAATGCTAAGCATGGACTGCGCTGCAAA
GCCTGTAAGATGAGCATCCACCACAAGTGCACAGATGGCCTGGCACCCCAGCGGTGCATGGGCAAGCTGC
CAAAGGGGTTTCGGCGTTACTACAGCTCCCCCTTGCTCATTCATGAACAGTTTGGCTGCATTAAAGAAGT
TATGCCCATTGCCTGTGGCAATAAGGTGGACCCTGTCTACGAGACCCTCCGCTTCGGCACCTCCCTGGCC
CAGAGGACAAAGAAGGGCAGCTCCGGCAGTGGCTCTGACTCACCTCACAGAACCTCTACTTCAGATCTTG
TGGAGGTTCCTGAGGAAGCCAATGGGCCAGGAGGCGGGTATGACCTAAGGAAACGCAGCAACAGCGTGTT
TACATATCCAGAAAATGGCACTGATGATTTCAGAGATCCAGCGAAGAACATAAACCACCAGGGATCTCTT
TCCAAAGACCCATTACAGATGAACACCTATGTTGCCTTGTACAAATTTGTACCACAGGAGAATGAAGATT
TGGAAATGAGGCCAGGAGACATAATTACTCTTTTAGAGGATTCCAATGAAGACTGGTGGAAAGGGAAAAT
TCAAGACAGAATTGGCTTCTTTCCAGCCAACTTTGTTCAGAGACTACAACAAAATGAGAAGATTTTTAGA
TGTGTTAGAACCTTCATTGGGTGTAAGGAACAGGGGCAGATAACACTGAAAGAGAATCAGATCTGCGTGA
GTTCTGAAGAAGAACAAGATGGTTTTATCAGAGTCCTCAGTGGAAAAAAGAAAGGCCTCATCCCCCTTGA
TGTACTAGAAAACATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205851 protein sequence
Red=Cloning site Green=Tags(s)

MIPPSSPREDGVDGLPKEAVGAEQPPSPASTSSQESKLQKLKRSLSFKTKSLRSKSADNFFQRTNSEDMK
LQAHMVAEISPSSSPLPAPGSLTSTPARAGLHPGGKAHAFHEYIFKKPTFCDVCNHMIVGTNAKHGLRCK
ACKMSIHHKCTDGLAPQRCMGKLPKGFRRYYSSPLLIHEQFGCIKEVMPIACGNKVDPVYETLRFGTSLA
QRTKKGSSGSGSDSPHRTSTSDLVEVPEEANGPGGGYDLRKRSNSVFTYPENGTDDFRDPAKNINHQGSL
SKDPLQMNTYVALYKFVPQENEDLEMRPGDIITLLEDSNEDWWKGKIQDRIGFFPANFVQRLQQNEKIFR
CVRTFIGCKEQGQITLKENQICVSSEEEQDGFIRVLSGKKKGLIPLDVLENI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003149
ORF Size 1206 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003149.2
RefSeq Size 3098 bp
RefSeq ORF 1209 bp
Locus ID 6769
UniProt ID Q8WUK8, Q99469
Cytogenetics 3p22.3-p22.2
Domains DAG_PE-bind, SH3
MW 44.6 kDa
Summary Promotes expression of the ion channel CACNA1H at the cell membrane, and thereby contributes to the regulation of channel activity. Plays a minor and redundant role in promoting the expression of calcium channel CACNA1S at the cell membrane, and thereby contributes to increased channel activity. Slows down the inactivation rate of the calcium channel CACNA1C.UniProtKB/Swiss-Prot Function
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"NM_003149" in other vectors (4)
SKU Description Size Price
RC205851L3 Lenti ORF clone of Human SH3 and cysteine rich domain (STAC), Myc-DDK-tagged 10 ug
$757.00
RC205851L4 Lenti ORF clone of Human SH3 and cysteine rich domain (STAC), mGFP tagged 10 ug
$757.00
RG205851 STAC (tGFP-tagged) - Human SH3 and cysteine rich domain (STAC) 10 ug
$489.00 $657.00
SC319517 STAC (untagged)-Human SH3 and cysteine rich domain (STAC) 10 ug
$457.00

Plasmid Map for RC205851

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205851
Circular map for RC205851

file-alt Citations

Allosteric regulators selectively prevent Ca2+-feedback of CaV and NaV channels
Niu, Dick, Yang et al
Elife (2018) 7 DOI: 10.7554/eLife.35222
AI Summary: OriGene products, specifically stac1, stac2, and stac3, were used as sources of gene constructs to investigate how these proteins selectively modulate Ca2+/calmodulin regulation of CaV and NaV channels in the study.
Snippet: aV2.2 PMID: 1321501, 10233069 GenBank: M94172.1 Gene (rat) CaV2.3 PMID: 8388125, 18400181 NCBI: NM_019294.2 Gene (rat) NaV1.4 PMID: 2175278 Gene (human) stac1 Origene NCBI: NP_003140.1 Gene (mouse) stac2 Origene NCBI: NP_666140.1 Gene (human) stac3 Origene NCBI: NP_659501.1 Gene (human) fhf PMID: 8790420 Laboratory
FigureFigureFigure
Showing 1-1 of 1 papers.
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