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FBXW2 (NM_012164) Human Tagged ORF Clone

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SKU
RC205840
FBXW2 (Myc-DDK-tagged)-Human F-box and WD repeat domain containing 2 (FBXW2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FBXW2
Synonyms FBW2; Fwd2; Md6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205840 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAGAAAGGACTTTGAGACATGGCTTGATAACATTTCTGTTACATTTCTTTCTCTGACGGACTTGC
AGAAAAATGAAACTCTGGATCACCTGATTAGTCTGAGTGGGGCAGTCCAGCTCAGGCATCTCTCCAATAA
CCTAGAGACTCTCCTCAAGCGGGACTTCCTCAAACTCCTTCCCCTGGAGCTCAGTTTTTATTTGTTAAAA
TGGCTCGATCCTCAGACTTTACTCACATGCTGCCTCGTCTCTAAACAGTGGAATAAGGTGATAAGTGCCT
GTACAGAGGTGTGGCAGACTGCATGTAAAAATTTGGGCTGGCAGATAGATGATTCTGTTCAGGACGCTTT
GCACTGGAAGAAGGTTTATTTGAAGGCTATTTTGAGAATGAAGCAACTGGAGGACCATGAAGCCTTTGAA
ACCTCGTCATTAATTGGACACAGTGCCAGAGTGTATGCACTTTACTACAAAGATGGACTTCTCTGTACAG
GGTCAGATGACTTGTCTGCAAAGCTGTGGGATGTGAGCACAGGGCAGTGCGTTTATGGCATCCAGACCCA
CACTTGTGCAGCGGTGAAGTTTGATGAACAGAAGCTTGTGACAGGCTCCTTTGACAACACTGTGGCTTGC
TGGGAATGGAGTTCCGGAGCCAGGACCCAGCACTTTCGGGGGCACACGGGGGCGGTATTTAGCGTGGACT
ACAATGATGAACTGGATATCTTGGTGAGCGGCTCTGCAGACTTCACTGTGAAAGTATGGGCTTTATCTGC
TGGGACATGCCTGAACACACTCACCGGGCACACGGAATGGGTCACCAAGGTAGTTTTGCAGAAGTGCAAA
GTCAAGTCTCTCTTGCACAGTCCTGGAGACTACATCCTCTTAAGTGCAGACAAATATGAGATTAAGATTT
GGCCAATTGGGAGAGAAATCAACTGTAAGTGCTTAAAGACATTGTCTGTCTCTGAGGATAGAAGTATCTG
CCTGCAGCCAAGACTTCATTTTGATGGCAAATACATTGTCTGTAGTTCAGCACTTGGTCTCTACCAGTGG
GACTTTGCCAGTTATGATATTCTCAGGGTCATCAAGACTCCTGAGATAGCAAACTTGGCCTTGCTTGGCT
TTGGAGATATCTTTGCCCTGCTGTTTGACAACCGCTACCTGTACATCATGGACTTGCGGACAGAGAGCCT
GATTAGTCGCTGGCCTCTGCCAGAGTACAGGAAATCAAAGAGAGGCTCAAGCTTCCTGGCAGGCGAAGCA
TCCTGGCTGAATGGACTGGATGGGCACAATGACACGGGCTTGGTCTTTGCCACCAGCATGCCTGACCACA
GTATTCACCTGGTGTTGTGGAAGGAGCACGGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205840 protein sequence
Red=Cloning site Green=Tags(s)

MERKDFETWLDNISVTFLSLTDLQKNETLDHLISLSGAVQLRHLSNNLETLLKRDFLKLLPLELSFYLLK
WLDPQTLLTCCLVSKQWNKVISACTEVWQTACKNLGWQIDDSVQDALHWKKVYLKAILRMKQLEDHEAFE
TSSLIGHSARVYALYYKDGLLCTGSDDLSAKLWDVSTGQCVYGIQTHTCAAVKFDEQKLVTGSFDNTVAC
WEWSSGARTQHFRGHTGAVFSVDYNDELDILVSGSADFTVKVWALSAGTCLNTLTGHTEWVTKVVLQKCK
VKSLLHSPGDYILLSADKYEIKIWPIGREINCKCLKTLSVSEDRSICLQPRLHFDGKYIVCSSALGLYQW
DFASYDILRVIKTPEIANLALLGFGDIFALLFDNRYLYIMDLRTESLISRWPLPEYRKSKRGSSFLAGEA
SWLNGLDGHNDTGLVFATSMPDHSIHLVLWKEHG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012164
ORF Size 1362 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012164.4
RefSeq Size 9186 bp
RefSeq ORF 1365 bp
Locus ID 26190
UniProt ID Q9UKT8
Cytogenetics 9q33.2
Domains F-box, WD40
Protein Families Druggable Genome
MW 51.5 kDa
Summary F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins. In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins. Mammalian F-box proteins are classified into three groups based on the presence of either WD-40 repeats, leucine-rich repeats, or the presence or absence of other protein-protein interacting domains. This gene encodes the second identified member of the F-box gene family and contains multiple WD-40 repeats. provided by RefSeq, Jul 2008
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"NM_012164" in other vectors (7)
SKU Description Size Price
RC205840L1 Lenti ORF clone of Human F-box and WD repeat domain containing 2 (FBXW2), Myc-DDK-tagged 10 ug
$757.00
RC205840L2 Lenti ORF clone of Human F-box and WD repeat domain containing 2 (FBXW2), mGFP tagged 10 ug
$757.00
RC205840L3 Lenti ORF clone of Human F-box and WD repeat domain containing 2 (FBXW2), Myc-DDK-tagged 10 ug
$757.00
RC205840L4 Lenti ORF clone of Human F-box and WD repeat domain containing 2 (FBXW2), mGFP tagged 10 ug
$757.00
RG205840 FBXW2 (tGFP-tagged) - Human F-box and WD repeat domain containing 2 (FBXW2) 10 ug
$489.00 $657.00
SC115470 FBXW2 (untagged)-Human F-box and WD repeat domain containing 2 (FBXW2) 10 ug
$457.00
SC317741 FBXW2 (untagged)-Human F-box and WD repeat domain containing 2 (FBXW2) 10 ug
$503.00

Plasmid Map for RC205840

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205840
Circular map for RC205840

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