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FBXW2 (NM_012164) Human Untagged Clone

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SKU
SC115470
FBXW2 (untagged)-Human F-box and WD repeat domain containing 2 (FBXW2)
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol FBXW2
Synonyms FBW2; Fwd2; Md6
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC115470 sequence for NM_012164 edited (data generated by NextGen Sequencing)
ATGGAGAGAAAGGACTTTGAGACATGGCTTGATAACATTTCTGTTACATTTCTTTCTCTG
ACGGACTTGCAGAAAAATGAAACTCTGGATCACCTGATTAGTCTGAGTGGGGCAGTCCAG
CTCAGGCATCTCTCCAATAACCTAGAGACTCTCCTCAAGCGGGACTTCCTCAAACTCCTT
CCCCTGGAGCTCAGTTTTTATTTGTTAAAATGGCTCGATCCTCAGACTTTACTCACATGC
TGCCTCGTCTCTAAACAGTGGAATAAGGTGATAAGTGCCTGTACAGAGGTGTGGCAGACT
GCATGTAAAAATTTGGGCTGGCAGATAGATGATTCTGTTCAGGACGCTTTGCACTGGAAG
AAGGTTTATTTGAAGGCTATTTTGAGAATGAAGCAACTGGAGGACCATGAAGCCTTTGAA
ACCTCGTCATTAATTGGACACAGTGCCAGAGTGTATGCACTTTACTACAAAGATGGACTT
CTCTGTACAGGGTCAGATGACTTGTCTGCAAAGCTGTGGGATGTGAGCACAGGGCAGTGC
GTTTATGGCATCCAGACCCACACTTGTGCAGCGGTGAAGTTTGATGAACAGAAGCTTGTG
ACAGGCTCCTTTGACAACACTGTGGCTTGCTGGGAATGGAGTTCCGGAGCCAGGACCCAG
CACTTTCGGGGGCACACGGGGGCGGTATTTAGCGTGGACTACAATGATGAACTGGATATC
TTGGTGAGCGGCTCTGCAGACTTCACTGTGAAAGTATGGGCTTTATCTGCTGGGACATGC
CTGAACACACTCACCGGGCACACGGAATGGGTCACCAAGGTAGTTTTGCAGAAGTGCAAA
GTCAAGTCTCTCTTGCACAGTCCTGGAGACTACATCCTCTTAAGTGCAGACAAATATGAG
ATTAAGATTTGGCCAATTGGGAGAGAAATCAACTGTAAGTGCTTAAAGACATTGTCTGTC
TCTGAGGATAGAAGTATCTGCCTGCAGCCAAGACTTCATTTTGATGGCAAATACATTGTC
TGTAGTTCAGCACTTGGTCTCTACCAGTGGGACTTTGCCAGTTATGATATTCTCAGGGTC
ATCAAGACTCCTGAGATAGCAAACTTGGCCTTGCTTGGCTTTGGAGATATCTTTGCCCTG
CTGTTTGACAACCGCTACCTGTACATCATGGACTTGCGGACAGAGAGCCTGATTAGTCGC
TGGCCTCTGCCAGAGTACAGGAAATCAAAGAGAGGCTCAAGCTTCCTGGCAGGCGAAGCA
TCCTGGCTGAATGGACTGGATGGGCACAATGACACGGGCTTGGTCTTTGCCACCAGCATG
CCTGACCACAGTATTCACCTGGTGTTGTGGAAGGAGCACGGCTGA

Clone variation with respect to NM_012164.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_012164 unedited
TTCAAATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGACGCAG
GCAGCNACCGGAGCCGCTCCCCTGTGAGGCTGCGGACCGGGAGCAGCGGCCGCAGGCGCC
ATGGCTGCGGAGCGGACCCGGCCGCTGCAAGGCTCTGGCGGTCCGAGCGTGCCTAGTAGC
TGTGAACCCGGCGCGAGGTCCCGGGCCCCGGGGCGCTCGCTCAGGTAAATTTTTCCATAA
CCTTATGGAGAGAAAGGACTTTGAGACATGGCTTGATAACATTTCTGTTACATTTCTTTC
TCTGACGGACTTGCAGAAAAATGAAACTCTGGATCACCTGATTAGTCTGAGTGGGGCAGT
CCAGCTCAGGCATCTCTCCAATAACCTAGAGACTCTCCTCAAGCGGGACTTCCTCAAACT
CCTTCCCCTGGAGCTCAGTTTTTATTTGTTAAAATGGCTCGATCCTCAGACTTTACTCAC
ATGCTGCCTCGTCTCTAAACAGTGGAATAAGGTGATAAGTGCCTGTACAGAGGTGTGGCA
GACTGCATGTAAAAATTTGGGCTGGCAGATAGATGATTCTGTTCAGGACGCTTTGCACTG
GAAGAAGGTTTATTTGAAGGCTATTTTGAGAATGAAGCAACTGGAGGACCATGAAGCCTT
TGAAACCTCGTCATTAATTGGACACAGTGCCAGAGTGTATGCACTNTACTACAAAGATGG
ACTTCTCTGTACAGGGTCAGATGACTTGTCTGCANAGCTGTGGGATGTGAGCACAGGGCA
GTGCGTTTATGGCATCCAGACCCACACTTGTGCAGCGGTGAAAGTTTGATGAACAGAAGC
TTGTGACAGGCTCCTTTGACACACTGTGGCTTGCTGGNAATGGAGTCCGGAGCCAGGACC
CACACTTTCGGGGCACACGGGGCCGTATTTAGCGC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_012164 unedited
NNNNTTTTACTCTGGACCCGCGGCCGCATNCTANGANCGAGTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAATTAAACTTTTTTTACTTAA
AACCAATACTCCACTCAAAAAAAATGAACCCAAAAGGCATCCTTTAACCCTGGCCAACAT
AACTAAGTCCAAAGGAAGCCAAGGGGGGCCCCCATTAACATGCTGGGTTGTATGTAAAAA
AAAAAAGCCCTCCCCCACCCCGAGCCCTGGCCCCTGGAAAAAAATAAAAAAAGGATTGGG
CCCTGGGGGAAGAAACCATTAGGGGAAAACTTTGGTTAATGCAGTCGGTTGCCGAAAAGG
TTGCACCCAAAACCCGAAGCCCCGGCACCCAAAGTAAGTAAGGGGGGGGGGTTAATGGGG
TAAACCGGGCTCCTTCCCAACACCAGGGGAATACTGGGGTCAGGCATGCTGGGGGAAAAA
ACCAACCCCGGGTCATTGGGCCCATCCAGTCCATTCAGCCAGGATGTTTCCCCTGCCAAA
AAGCTTGAGCCTCTCTTTGATTCCCTGAACTCGGGCAGAGGCCAGCGACTAATCAGGCTC
TCTGTCCGCAAGTCCATGATGGACAGGGAAGCGGGGTGACAAACAACAGGGGCAAAGAAT
TTTCCCAAAGCCAAGCAAGGGCCAAGTTTTGCTATTTTCAGGAAGCTTGGAGGACCCCTG
GAAAATATCAAAACGGGGCAAAGCCCCCCTGGGAAGAAAACCAAGGGGCGAAAAAACAGA
ACAAGGGGTTTTGCCCTAAAAAAGAAGAATTTTGGGGGTGGGGGACAAAACCTTTTTTCC
TCGAAAACAAAAAAGGTTTTAAGACCTTCAGCGGGGGTTTCTTCCCCAAAGGGGGCCAAA
CTTAATCCTATATTGGGGCGGCCTTAAAGGGGGAGGACCCCCCAA
Restriction Sites NotI-NotI |
ACCN NM_012164
Insert Size 1910 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012164.2, NP_036296.1
RefSeq Size 1886 bp
RefSeq ORF 1269 bp
Locus ID 26190
UniProt ID Q9UKT8
Cytogenetics 9q33.2
Domains F-box, WD40
Protein Families Druggable Genome
Summary F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins. In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins. Mammalian F-box proteins are classified into three groups based on the presence of either WD-40 repeats, leucine-rich repeats, or the presence or absence of other protein-protein interacting domains. This gene encodes the second identified member of the F-box gene family and contains multiple WD-40 repeats. provided by RefSeq, Jul 2008
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"NM_012164" in other vectors (7)
SKU Description Size Price
RC205840 FBXW2 (Myc-DDK-tagged)-Human F-box and WD repeat domain containing 2 (FBXW2) 10 ug
$457.00
RC205840L1 Lenti ORF clone of Human F-box and WD repeat domain containing 2 (FBXW2), Myc-DDK-tagged 10 ug
$757.00
RC205840L2 Lenti ORF clone of Human F-box and WD repeat domain containing 2 (FBXW2), mGFP tagged 10 ug
$757.00
RC205840L3 Lenti ORF clone of Human F-box and WD repeat domain containing 2 (FBXW2), Myc-DDK-tagged 10 ug
$757.00
RC205840L4 Lenti ORF clone of Human F-box and WD repeat domain containing 2 (FBXW2), mGFP tagged 10 ug
$757.00
RG205840 FBXW2 (tGFP-tagged) - Human F-box and WD repeat domain containing 2 (FBXW2) 10 ug
$489.00 $657.00
SC317741 FBXW2 (untagged)-Human F-box and WD repeat domain containing 2 (FBXW2) 10 ug
$503.00

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