AMDHD2 (NM_015944) Human Tagged ORF Clone

  • Product Brand Image
SKU
RC205838
AMDHD2 (Myc-DDK-tagged)-Human amidohydrolase domain containing 2 (AMDHD2), transcript variant 1
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol AMDHD2
Synonyms CGI-14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205838 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCGGCGAGCAGGGCGCGGCGGGGGCCCGCGTGCTCCAGTTCACTAACTGCCGGATCCTGCGCGGAG
GGAAACTGCTCAGGGAGGATCTGTGGGTGCGCGGAGGCCGCATCTTGGACCCAGAGAAGCTGTTCTTTGA
GGAGCGGCGCGTGGCCGACGAGCGGCGGGACTGCGGGGGCCGCATCTTGGCTCCCGGATTCATCGACGTG
CAGATCAACGGTGGATTTGGTGTTGACTTCTCTCAAGCCACGGAGGACGTGGGTTCGGGGGTTGCCCTCG
TGGCCCGGAGGATCCTGTCGCACGGCGTCACCTCCTTCTGCCCCACCCTGGTCACTTCCCCACCGGAGGT
TTATCACAAGGTTGTTCCTCAGATCCCTGTGAAGAGTGGTGGTCCCCATGGGGCAGGGGTCCTCGGGCTG
CACCTGGAGGGCCCCTTCATCAGCCGGGAGAAGCGGGGCGCGCACCCCGAGGCCCACCTCCGCTCCTTCG
AGGCCGATGCCTTCCAGGACTTGCTGGCCACCTACGGGCCCCTGGACAATGTCCGCATCGTGACGCTGGC
CCCAGAGTTGGGCCGTAGCCACGAAGTGATCCGGGCGCTGACGGCCCGTGGCATCTGCGTGTCCCTAGGG
CACTCAGTGGCTGACCTGCGGGCGGCAGAGGATGCTGTGTGGAGCGGAGCCACCTTCATCACCCACCTCT
TCAACGCCATGCTGCCTTTCCACCACCGCGACCCAGGCATCGTGGGGCTCCTGACCAGCGACCGGCTGCC
CGCAGGCCGCTGCATCTTCTATGGGATGATTGCAGATGGCACGCACACCAACCCCGCCGCCCTGCGGATC
GCCCACCGTGCCCATCCCCAGGGGCTGGTGCTGGTCACCGATGCCATCCCTGCCTTGGGCCTGGGCAACG
GCCGGCACACGCTGGGACAGCAGGAAGTGGAAGTGGACGGTCTGACGGCCTACGTGGCAGGTGAGCGCCC
TGACCCACTGGGTCCCAGGTCCCAGCCCGCATGCCAGGTGGCCCACGACCCCCCCAGAGCCTGCCCTCTC
TGCTCTCAAGGCACCAAGACGCTGAGTGGCAGCATAGCCCCAATGGACGTCTGTGTCCGGCACTTCCTGC
AGGCCACAGGCTGCAGCATGGAGTCGGCCCTGGAGGCTGCATCCCTGCACCCCGCCCAGTTGCTGGGGCT
GGAGAAGAGTAAGGGGACCCTGGACTTTGGTGCTGACGCAGACTTCGTGGTGCTCGACGACTCCCTTCAC
GTCCAGGCCACCTACATCTCGGGTGAGCTGGTGTGGCAGGCGGACGCAGCTAGGCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC205838 protein sequence
Red=Cloning site Green=Tags(s)

MRGEQGAAGARVLQFTNCRILRGGKLLREDLWVRGGRILDPEKLFFEERRVADERRDCGGRILAPGFIDV
QINGGFGVDFSQATEDVGSGVALVARRILSHGVTSFCPTLVTSPPEVYHKVVPQIPVKSGGPHGAGVLGL
HLEGPFISREKRGAHPEAHLRSFEADAFQDLLATYGPLDNVRIVTLAPELGRSHEVIRALTARGICVSLG
HSVADLRAAEDAVWSGATFITHLFNAMLPFHHRDPGIVGLLTSDRLPAGRCIFYGMIADGTHTNPAALRI
AHRAHPQGLVLVTDAIPALGLGNGRHTLGQQEVEVDGLTAYVAGERPDPLGPRSQPACQVAHDPPRACPL
CSQGTKTLSGSIAPMDVCVRHFLQATGCSMESALEAASLHPAQLLGLEKSKGTLDFGADADFVVLDDSLH
VQATYISGELVWQADAARQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015944
ORF Size 1317 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015944.4
RefSeq Size 1604 bp
RefSeq ORF 1320 bp
Locus ID 51005
UniProt ID Q9Y303, Q9Y303-2
Cytogenetics 16p13.3
Domains Amidohydro_1
Protein Pathways Amino sugar and nucleotide sugar metabolism
MW 46.9 kDa
Summary Hydrolyzes the N-glycolyl group from N-glycolylglucosamine 6-phosphate (GlcNGc-6-P) in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway. Although human is not able to catalyze formation of Neu5Gc due to the inactive CMAHP enzyme, Neu5Gc is present in food and must be degraded.UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_015944" in other vectors (4)
SKU Description Size Price
RC205838L3 Lenti ORF clone of Human amidohydrolase domain containing 2 (AMDHD2), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC205838L4 Lenti ORF clone of Human amidohydrolase domain containing 2 (AMDHD2), transcript variant 1, mGFP tagged 10 ug
$757.00
RG205838 AMDHD2 (tGFP-tagged) - Human amidohydrolase domain containing 2 (AMDHD2), transcript variant 1 10 ug
$489.00 $657.00
SC319557 AMDHD2 (untagged)-Human amidohydrolase domain containing 2 (AMDHD2), transcript variant 1 10 ug
$457.00

Plasmid Map for RC205838

Download sequence for

Cloning scheme for RC205838

Circular map for RC205838

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products