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MRPL2 (NM_015950) Human Tagged ORF Clone

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SKU
RC205836
MRPL2 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L2 (MRPL2), nuclear gene encoding mitochondrial protein
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MRPL2
Synonyms CGI-22; MRP-L14; RPML14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205836 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTGTGCGCACTGACCCGCGCTCTGCGCTCTCTGAACCTGGCGCCCCCGACCGTCGCCGCCCCTG
CCCCGAGTCTGTTCCCCGCCGCCCAGATGATGAACAATGGCCTCCTCCAACAGCCCTCTGCCTTGATGTT
GCTCCCCTGCCGCCCAGTTCTTACTTCTGTGGCCCTTAATGCCAACTTTGTGTCCTGGAAGAGTCGTACC
AAGTACACCATTACACCAGTGAAGATGAGGAAGTCTGGGGGCCGAGACCACACAGGCCGAATCCGGGTGC
ATGGTATTGGCGGGGGCCACAAGCAACGTTATCGAATGATTGACTTTCTGCGTTTCCGGCCTGAGGAGAC
CAAGTCAGGACCCTTTGAGGAGAAGGTTATCCAAGTCCGCTATGATCCCTGTAGGTCAGCAGACATAGCT
CTGGTTGCTGGGGGCAGCCGGAAACGCTGGATCATCGCCACAGAAAACATGCAGGCTGGAGATACAATCT
TGAACTCTAACCACATAGGCCGAATGGCAGTTGCTGCTCGGGAAGGGGATGCGCATCCTCTTGGGGCTCT
GCCTGTGGGGACCCTCATCAACAACGTGGAAAGTGAGCCAGGCCGGGGTGCCCAATATATCCGAGCTGCA
GGGACGTGTGGTGTGCTACTGCGGAAGGTGAATGGCACAGCCATTATCCAGCTGCCCTCTAAGAGGCAGA
TGCAGGTGCTGGAAACGTGCGTAGCAACAGTAGGCCGAGTATCCAACGTTGATCATAACAAACGGGTCAT
TGGCAAGGCAGGTCGCAACCGCTGGCTGGGCAAGAGGCCTAACAGTGGGCGGTGGCACCGCAAGGGGGGC
TGGGCTGGCCGAAAGATTCGGCCACTACCCCCCATGAAGAGTTACGTGAAGCTGCCTTCTGCTTCTGCCC
AAAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205836 protein sequence
Red=Cloning site Green=Tags(s)

MALCALTRALRSLNLAPPTVAAPAPSLFPAAQMMNNGLLQQPSALMLLPCRPVLTSVALNANFVSWKSRT
KYTITPVKMRKSGGRDHTGRIRVHGIGGGHKQRYRMIDFLRFRPEETKSGPFEEKVIQVRYDPCRSADIA
LVAGGSRKRWIIATENMQAGDTILNSNHIGRMAVAAREGDAHPLGALPVGTLINNVESEPGRGAQYIRAA
GTCGVLLRKVNGTAIIQLPSKRQMQVLETCVATVGRVSNVDHNKRVIGKAGRNRWLGKRPNSGRWHRKGG
WAGRKIRPLPPMKSYVKLPSASAQS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015950
ORF Size 915 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015950.5
RefSeq Size 1333 bp
RefSeq ORF 918 bp
Locus ID 51069
UniProt ID Q5T653
Cytogenetics 6p21.1
MW 33.3 kDa
Summary Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 12q. Alternative splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Jul 2014
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"NM_015950" in other vectors (5)
SKU Description Size Price
RC205836L3 Lenti ORF clone of Human mitochondrial ribosomal protein L2 (MRPL2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$600.00
RC205836L4 Lenti ORF clone of Human mitochondrial ribosomal protein L2 (MRPL2), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$600.00
RG205836 MRPL2 (tGFP-tagged) - Human mitochondrial ribosomal protein L2 (MRPL2), nuclear gene encoding mitochondrial protein 10 ug
$500.00
SC304370 MRPL2 (untagged)-Human mitochondrial ribosomal protein L2 (MRPL2), nuclear gene encoding mitochondrial protein 10 ug
$330.00
SC320944 MRPL2 (untagged)-Human mitochondrial ribosomal protein L2 (MRPL2), nuclear gene encoding mitochondrial protein 10 ug
$300.00

Plasmid Map for RC205836

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205836
Circular map for RC205836

file-alt Citations

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