SNIP1 (NM_024700) Human Tagged ORF Clone

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SKU
RC205787
SNIP1 (Myc-DDK-tagged)-Human Smad nuclear interacting protein 1 (SNIP1)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SNIP1
Synonyms PML1; PMRED
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205787 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGCGGTGAAGAGCGAACGGGAGCGAGGGAGCCGGCGAAGACACCGGGACGGGGACGTGGTGCTGC
CGGCGGGGGTGGTAGTGAAGCAGGAGCGTCTCAGCCCAGAAGTCGCACCTCCCGCCCACCGCCGTCCGGA
CCACTCCGGTGGTAGCCCGTCTCCGCCGACCAGCGAGCCGGCCCGCTCGGGCCACCGCGGGAACCGAGCC
CGAGGAGTTAGCCGGTCCCCACCCAAAAAGAAAAACAAGGCCTCAGGGAGAAGAAGCAAGTCTCCTCGCA
GTAAGAGAAACCGAAGTCCTCACCACTCAACAGTCAAAGTGAAGCAGGAGCGTGAGGATCATCCCCGGAG
AGGACGGGAGGATCGGCAGCACAGGGAACCATCAGAACAGGAACACAGGAGAGCTAGGAACAGTGACCGG
GACAGACACCGGGGCCATTCCCACCAAAGGAGAACGTCTAACGAGAGGCCTGGGAGTGGGCAGGGTCAGG
GACGGGATCGAGACACTCAGAACCTGCAGGCTCAGGAAGAAGAGCGGGAGTTTTATAATGCCAGGCGACG
GGAGCATCGCCAGAGGAATGACGTTGGTGGTGGCGGCAGTGAGTCTCAGGAGTTGGTTCCTCGGCCTGGT
GGCAACAACAAAGAAAAAGAGGTGCCCGCTAAAGAAAAACCAAGCTTTGAACTTTCTGGGGCACTTCTTG
AGGACACCAACACTTTCCGGGGTGTAGTCATTAAATATAGTGAGCCCCCAGAAGCACGTATCCCCAAAAA
ACGGTGGCGTCTCTACCCATTTAAAAATGATGAGGTGCTTCCAGTCATGTACATACATCGACAGAGTGCG
TACCTACTGGGTCGACACCGCCGCATTGCAGACATTCCAATTGATCACCCGTCTTGTTCAAAGCAGCATG
CGGTCTTTCAATATCGGCTTGTGGAATATACCCGTGCTGATGGCACAGTTGGCCGAAGAGTGAAGCCCTA
CATCATTGACCTTGGCTCAGGCAATGGAACCTTCTTAAACAACAAACGTATTGAGCCACAGAGATACTAT
GAACTAAAAGAAAAGGATGTACTCAAATTTGGATTCAGTAGCAGAGAATACGTCTTGCTCCATGAGTCGT
CGGACACTTCTGAAATAGACAGGAAAGATGACGAGGATGAGGAGGAGGAGGAAGAAGTGTCTGACAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC205787 protein sequence
Red=Cloning site Green=Tags(s)

MKAVKSERERGSRRRHRDGDVVLPAGVVVKQERLSPEVAPPAHRRPDHSGGSPSPPTSEPARSGHRGNRA
RGVSRSPPKKKNKASGRRSKSPRSKRNRSPHHSTVKVKQEREDHPRRGREDRQHREPSEQEHRRARNSDR
DRHRGHSHQRRTSNERPGSGQGQGRDRDTQNLQAQEEEREFYNARRREHRQRNDVGGGGSESQELVPRPG
GNNKEKEVPAKEKPSFELSGALLEDTNTFRGVVIKYSEPPEARIPKKRWRLYPFKNDEVLPVMYIHRQSA
YLLGRHRRIADIPIDHPSCSKQHAVFQYRLVEYTRADGTVGRRVKPYIIDLGSGNGTFLNNKRIEPQRYY
ELKEKDVLKFGFSSREYVLLHESSDTSEIDRKDDEDEEEEEEVSDS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024700
ORF Size 1188 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024700.2, NP_078976.2
RefSeq Size 4605 bp
RefSeq ORF 1191 bp
Locus ID 79753
UniProt ID Q8TAD8, B1AK66
Cytogenetics 1p34.3
Domains FHA
MW 45.8 kDa
Summary This gene encodes a protein that contains a coiled-coil motif and C-terminal forkhead-associated (FHA) domain. The encoded protein functions as a transcriptional coactivator that increases c-Myc activity and inhibits transforming growth factor beta (TGF-beta) and nuclear factor kappa-B (NF-kB) signaling. The encoded protein also regulates the stability of cyclin D1 mRNA, and may play a role in cell proliferation and cancer progression. Mutations in this gene are a cause of psychomotor retardation, epilepsy, and craniofacial dysmorphism (PMRED). provided by RefSeq, Mar 2012
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Documents
Resources
"NM_024700" in other vectors (6)
SKU Description Size Price
RC205787L1 Lenti ORF clone of Human Smad nuclear interacting protein 1 (SNIP1), Myc-DDK-tagged 10 ug
$757.00
RC205787L2 Lenti ORF clone of Human Smad nuclear interacting protein 1 (SNIP1), mGFP tagged 10 ug
$757.00
RC205787L3 Lenti ORF clone of Human Smad nuclear interacting protein 1 (SNIP1), Myc-DDK-tagged 10 ug
$757.00
RC205787L4 Lenti ORF clone of Human Smad nuclear interacting protein 1 (SNIP1), mGFP tagged 10 ug
$757.00
RG205787 SNIP1 (tGFP-tagged) - Human Smad nuclear interacting protein 1 (SNIP1) 10 ug
$489.00 $657.00
SC321705 SNIP1 (untagged)-Human Smad nuclear interacting protein 1 (SNIP1) 10 ug
$457.00

Plasmid Map for RC205787

Download sequence for

Cloning scheme for RC205787

Circular map for RC205787

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