TRIP6 (NM_003302) Human Tagged ORF Clone

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SKU
RC205778
TRIP6 (Myc-DDK-tagged)-Human thyroid hormone receptor interactor 6 (TRIP6)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TRIP6
Synonyms OIP-1; OIP1; TRIP-6; TRIP6i2; ZRP-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205778 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGGGCCCACCTGGCTGCCCCCGAAGCAGCCGGAGCCCGCCAGAGCCCCTCAGGGGAGGGCGATCC
CCCGCGGCACCCCGGGGCCACCACCGGCCCACGGAGCAGCACTCCAGCCCCACCCCAGGGTCAATTTTTG
CCCCCTTCCATCTGAGCAGTGTTACCAGGCCCCAGGGGGACCGGAGGATCGGGGGCCGGCGTGGGTGGGG
TCCCATGGAGTACTCCAGCACACGCAGGGGCTCCCTGCAGACAGGGGGGGCCTTCGCCCTGGAAGCCTGG
ACGCCGAGATAGACTTGCTGAGCAGCACGCTGGCCGAGCTGAATGGGGGTCGGGGTCATGCGTCACGGCG
ACCAGACCGACAGGCATATGAGCCCCCGCCACCTCCTGCCTACCGCACGGGCTCCCTGAAGCCAAATCCA
GCCTCGCCGCTCCCAGCGTCTCCCTATGGGGGCCCCACTCCAGCCTCTTACACTACCGCCAGCACCCCGG
CTGGCCCAGCCTTCCCCGTGCAAGTGAAGGTGGCACAGCCAGTGAGGGGCTGCGGCCCACCCAGGCGGGG
AGCCTCTCAGGCCTCTGGGCCCCTCCCGGGCCCCCACTTTCCTCTCCCAGGCCGAGGTGAAGTCTGGGGG
CCTGGCTATAGGAGCCAGAGAGAGCCAGGGCCAGGGGCCAAAGAGGAAGCTGCTGGGGTCTCTGGCCCTG
CAGGAAGAGGAAGAGGAGGCGAGCACGGGCCCCAGGTGCCCCTGAGCCAGCCTCCAGAGGATGAGCTGGA
TAGGCTGACGAAGAAGCTGGTTCACGACATGAACCACCCGCCCAGCGGGGAGTACTTTGGCCAGTGTGGT
GGCTGCGGAGAAGATGTGGTTGGGGATGGGGCTGGGGTTGTGGCCCTTGATCGCGTCTTTCACGTGGGCT
GCTTTGTATGTTCTACATGCCGGGCCCAGCTTCGCGGCCAGCATTTCTACGCCGTGGAGAGGAGGGCATA
TTGCGAGGGCTGCTACGTGGCCACCCTGGAGAAATGTGCCACGTGCTCCCAGCCCATCCTGGACCGGATC
CTGCGGGCTATGGGGAAGGCCTACCACCCTGGCTGCTTCACCTGCGTGGTGTGTCACCGCGGCCTCGACG
GCATCCCCTTCACAGTGGATGCTACGAGCCAGATCCACTGCATTGAGGACTTTCACAGGAAGTTTGCCCC
AAGATGCTCAGTGTGCGGTGGGGCCATAATGCCTGAGCCAGGTCAGGAGGAGACTGTGAGAATTGTTGCT
CTGGATCGAAGTTTTCACATTGGCTGTTACAAGTGCGAGGAGTGTGGGCTGCTGCTCTCCTCTGAGGGCG
AGTGTCAGGGCTGCTACCCGCTGGATGGGCACATCTTGTGCAAGGCCTGCAGCGCCTGGCGCATCCAGGA
GCTCTCAGCCACCGTCACCACTGACTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC205778 protein sequence
Red=Cloning site Green=Tags(s)

MSGPTWLPPKQPEPARAPQGRAIPRGTPGPPPAHGAALQPHPRVNFCPLPSEQCYQAPGGPEDRGPAWVG
SHGVLQHTQGLPADRGGLRPGSLDAEIDLLSSTLAELNGGRGHASRRPDRQAYEPPPPPAYRTGSLKPNP
ASPLPASPYGGPTPASYTTASTPAGPAFPVQVKVAQPVRGCGPPRRGASQASGPLPGPHFPLPGRGEVWG
PGYRSQREPGPGAKEEAAGVSGPAGRGRGGEHGPQVPLSQPPEDELDRLTKKLVHDMNHPPSGEYFGQCG
GCGEDVVGDGAGVVALDRVFHVGCFVCSTCRAQLRGQHFYAVERRAYCEGCYVATLEKCATCSQPILDRI
LRAMGKAYHPGCFTCVVCHRGLDGIPFTVDATSQIHCIEDFHRKFAPRCSVCGGAIMPEPGQEETVRIVA
LDRSFHIGCYKCEECGLLLSSEGECQGCYPLDGHILCKACSAWRIQELSATVTTDC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003302
ORF Size 1428 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003302.3
RefSeq Size 1762 bp
RefSeq ORF 1431 bp
Locus ID 7205
UniProt ID Q15654
Cytogenetics 7q22.1
Domains LIM
Protein Families Druggable Genome
Protein Pathways NOD-like receptor signaling pathway
MW 50.3 kDa
Summary This gene is a member of the zyxin family and encodes a protein with three LIM zinc-binding domains. This protein localizes to focal adhesion sites and along actin stress fibers. Recruitment of this protein to the plasma membrane occurs in a lysophosphatidic acid (LPA)-dependent manner and it regulates LPA-induced cell migration. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_003302" in other vectors (7)
SKU Description Size Price
RC205778L1 Lenti ORF clone of Human thyroid hormone receptor interactor 6 (TRIP6), Myc-DDK-tagged 10 ug
$757.00
RC205778L2 Lenti ORF clone of Human thyroid hormone receptor interactor 6 (TRIP6), mGFP tagged 10 ug
$757.00
RC205778L3 Lenti ORF clone of Human thyroid hormone receptor interactor 6 (TRIP6), Myc-DDK-tagged 10 ug
$757.00
RC205778L4 Lenti ORF clone of Human thyroid hormone receptor interactor 6 (TRIP6), mGFP tagged 10 ug
$757.00
RG205778 TRIP6 (tGFP-tagged) - Human thyroid hormone receptor interactor 6 (TRIP6) 10 ug
$489.00 $657.00
SC100311 TRIP6 (untagged)-Human thyroid hormone receptor interactor 6 (TRIP6) 10 ug
$457.00
SC322549 TRIP6 (untagged)-Human thyroid hormone receptor interactor 6 (TRIP6) 10 ug
$457.00

Plasmid Map for RC205778

Download sequence for

Cloning scheme for RC205778

Circular map for RC205778

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