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DR1 (NM_001938) Human Tagged ORF Clone

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SKU
RC205733
DR1 (Myc-DDK-tagged)-Human down-regulator of transcription 1, TBP-binding (negative cofactor 2) (DR1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DR1
Synonyms NC2; NC2-BETA; NC2B; NCB2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205733 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCCTCGTCTGGCAACGATGATGATCTCACTATCCCCAGAGCTGCTATCAATAAAATGATCAAAG
AGACTCTTCCTAATGTCCGGGTGGCCAACGATGCTCGAGAGCTGGTGGTGAACTGCTGCACTGAATTCAT
TCACCTTATATCTTCTGAAGCCAATGAGATTTGTAACAAATCGGAAAAGAAGACCATCTCACCAGAGCAT
GTCATACAAGCACTAGAAAGTTTGGGATTTGGCTCTTACATCAGTGAAGTAAAAGAAGTCTTGCAAGAGT
GTAAAACAGTAGCATTAAAAAGAAGAAAGGCCAGTTCTCGTTTGGAAAACCTTGGCATTCCTGAAGAAGA
GTTATTGAGACAGCAACAAGAATTATTTGCAAAAGCTAGACAGCAACAAGCAGAATTGGCCCAACAGGAA
TGGCTTCAAATGCAGCAAGCTGCCCAACAAGCCCAGCTTGCTGCTGCCTCAGCCAGTGCATCTAATCAGG
CGGGATCTTCTCAGGATGAAGAAGATGATGATGATATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205733 protein sequence
Red=Cloning site Green=Tags(s)

MASSSGNDDDLTIPRAAINKMIKETLPNVRVANDARELVVNCCTEFIHLISSEANEICNKSEKKTISPEH
VIQALESLGFGSYISEVKEVLQECKTVALKRRKASSRLENLGIPEEELLRQQQELFAKARQQQAELAQQE
WLQMQQAAQQAQLAAASASASNQAGSSQDEEDDDDI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001938
ORF Size 528 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001938.3
RefSeq Size 3222 bp
RefSeq ORF 531 bp
Locus ID 1810
UniProt ID Q01658, Q658N3
Cytogenetics 1p22.1
Domains CBFD_NFYB_HMF
Protein Families Transcription Factors
MW 19.4 kDa
Summary This gene encodes a TBP- (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The encoded protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. The binding of DR1 repressor complexes to TBP-promoter complexes may establish a mechanism in which an altered DNA conformation, together with the formation of higher order complexes, inhibits the assembly of the preinitiation complex and controls the rate of RNA polymerase II transcription. provided by RefSeq, Jul 2008
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"NM_001938" in other vectors (6)
SKU Description Size Price
RC205733L1 Lenti ORF clone of Human down-regulator of transcription 1, TBP-binding (negative cofactor 2) (DR1), Myc-DDK-tagged 10 ug
$600.00
RC205733L2 Lenti ORF clone of Human down-regulator of transcription 1, TBP-binding (negative cofactor 2) (DR1), mGFP tagged 10 ug
$600.00
RC205733L3 Lenti ORF clone of Human down-regulator of transcription 1, TBP-binding (negative cofactor 2) (DR1), Myc-DDK-tagged 10 ug
$600.00
RC205733L4 Lenti ORF clone of Human down-regulator of transcription 1, TBP-binding (negative cofactor 2) (DR1), mGFP tagged 10 ug
$600.00
RG205733 DR1 (tGFP-tagged) - Human down-regulator of transcription 1, TBP-binding (negative cofactor 2) (DR1) 10 ug
$489.00 $500.00
SC118958 DR1 (untagged)-Human down-regulator of transcription 1, TBP-binding (negative cofactor 2) (DR1) 10 ug
$300.00

Plasmid Map for RC205733

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205733
Circular map for RC205733

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