PCID1 (EIF3M) (NM_006360) Human Tagged ORF Clone

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SKU
RC205694
EIF3M (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit M (EIF3M)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PCID1
Synonyms B5; GA17; hfl-B5; PCID1; TANGO7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205694 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGTCCCGGCCTTCATCGACATCAGTGAAGAAGATCAGGCTGCTGAGCTTCGTGCTTATCTGAAAT
CTAAAGGAGCTGAGATTTCAGAAGAGAACTCGGAAGGTGGACTTCATGTTGATTTAGCTCAAATTATTGA
AGCCTGTGATGTGTGTCTGAAGGAGGATGATAAAGATGTTGAAAGTGTGATGAACAGTGTGGTATCCCTA
CTCTTGATCCTGGAACCAGACAAGCAAGAAGCTTTGATTGAAAGCCTATGTGAAAAGCTGGTCAAATTTC
GCGAAGGTGAACGCCCGTCTCTGAGACTGCAGTTGTTAAGCAACCTTTTCCACGGGATGGATAAGAATAC
TCCTGTAAGATACACAGTGTATTGCAGCCTTATTAAAGTGGCAGCATCTTGTGGGGCCATCCAGTACATC
CCAACTGAGCTGGATCAAGTTAGAAAATGGATTTCTGACTGGAATCTCACCACTGAAAAAAAGCACACCC
TTTTAAGACTACTTTATGAGGCACTTGTGGATTGTAAGAAGAGTGATGCTGCTTCAAAAGTCATGGTGGA
ATTGCTCGGAAGTTACACAGAGGACAATGCTTCCCAGGCTCGAGTTGATGCCCACAGGTGTATTGTACGA
GCATTGAAAGATCCAAATGCATTTCTTTTTGACCACCTTCTTACTTTAAAACCAGTCAAGTTTTTGGAAG
GCGAGCTTATTCATGATCTTTTAACCATTTTTGTGAGTGCTAAATTGGCATCATATGTCAAGTTTTATCA
GAATAATAAAGACTTCATTGATTCACTTGGCCTGTTACATGAACAGAATATGGCAAAAATGAGACTACTT
ACTTTTATGGGAATGGCAGTAGAAAATAAGGAAATTTCTTTTGACACAATGCAGCAAGAACTTCAGATTG
GAGCTGATGATGTTGAAGCATTTGTTATTGACGCCGTAAGAACTAAAATGGTCTACTGCAAAATTGATCA
GACCCAGAGAAAAGTAGTTGTCAGTCATAGCACACATCGGACATTTGGAAAACAGCAGTGGCAACAACTG
TATGACACACTTAATGCCTGGAAACAAAATCTGAACAAAGTGAAAAACAGCCTTTTGAGTCTTTCTGATA
CC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC205694 protein sequence
Red=Cloning site Green=Tags(s)

MSVPAFIDISEEDQAAELRAYLKSKGAEISEENSEGGLHVDLAQIIEACDVCLKEDDKDVESVMNSVVSL
LLILEPDKQEALIESLCEKLVKFREGERPSLRLQLLSNLFHGMDKNTPVRYTVYCSLIKVAASCGAIQYI
PTELDQVRKWISDWNLTTEKKHTLLRLLYEALVDCKKSDAASKVMVELLGSYTEDNASQARVDAHRCIVR
ALKDPNAFLFDHLLTLKPVKFLEGELIHDLLTIFVSAKLASYVKFYQNNKDFIDSLGLLHEQNMAKMRLL
TFMGMAVENKEISFDTMQQELQIGADDVEAFVIDAVRTKMVYCKIDQTQRKVVVSHSTHRTFGKQQWQQL
YDTLNAWKQNLNKVKNSLLSLSDT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006360
ORF Size 1122 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006360.6
RefSeq Size 1338 bp
RefSeq ORF 1125 bp
Locus ID 10480
UniProt ID Q7L2H7
Cytogenetics 11p13
Domains PCI
MW 42.5 kDa
Summary This gene encodes a protein that is part of the eurkaryotic translation initiation factor 3 complete (eIF-3) required for protein synthesis. Elevated levels of the encoded protein are present in cancer cell lines. Inactivation of the encoded protein has been shown to interfere with translation of herpes virus mRNAs by preventing the association of mRNAs with the ribosomes. A pseudogene of this gene is located on the X chromosome. provided by RefSeq, Dec 2011
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Documents
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"NM_006360" in other vectors (7)
SKU Description Size Price
RC205694L1 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit M (EIF3M), Myc-DDK-tagged 10 ug
$986.00
RC205694L2 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit M (EIF3M), mGFP tagged 10 ug
$986.00
RC205694L3 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit M (EIF3M), Myc-DDK-tagged 10 ug
$986.00
RC205694L4 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit M (EIF3M), mGFP tagged 10 ug
$986.00
RG205694 EIF3M (tGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit M (EIF3M) 10 ug
$886.00
SC116147 EIF3M (untagged)-Human eukaryotic translation initiation factor 3, subunit M (EIF3M) 10 ug
$686.00
SC320414 EIF3M (untagged)-Human eukaryotic translation initiation factor 3, subunit M (EIF3M) 10 ug
$686.00

Plasmid Map for RC205694

Download sequence for

Cloning scheme for RC205694

Circular map for RC205694

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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