EID3 (NM_001008394) Human Tagged ORF Clone

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SKU
RC205575
EID3 (Myc-DDK-tagged)-Human EP300 interacting inhibitor of differentiation 3 (EID3)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EID3
Synonyms NS4EB; NSE4B; NSMCE4B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205575 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGATGGATGTGTCAGTGAGGGCCGCGGGCTGCTCCGACGACCTCAGCTCTGGGGAGGCCGACGTAG
ACCCAAAGCTCCTGGAGCTCACCGCTGACGAGGAGAAGTGCCGCAGCATCCGCAGGCAGTACCGGCAGCT
CATGTACTGCGTGCGGCAGAACCGGGAGGACATCGTGAGCTCGGCGAACAACTCCTTAACCGAGGCTCTG
GAGGAAGCCAACGTCCTCTTTGATGGCGTGAGCCGAACCAGAGAAGCAGCCCTCGACGCCCGGTTTCTTG
TTATGGCTTCTGATTTGGGTAAAGAAAAGGCAAAGCAGTTAAACTCAGATATGAACTTCTTTAATCAGTT
AGCATTTTGTGACTTTCTGTTTCTGTTCGTGGGTCTGAATTGGATGGAAGGCGATCCTGACAAGTTGAGT
GATTGTGATGATAGCATAGCTCTTTCCTTCTGGAAGGCAATAGAAAAGGAAGCAACATCCTGGATGGTAA
AAGCTGAGACATTCCATTTTGTTTTTGGTTCATTCAAGCTAGAACGTTCTGCACCAAAGCCCCGACTTGA
ACACCAGAAAAAAGTTCGCAAGATGGAAGAAAATGGCAACATGCCTACAAAGTTGCAGAAGTTGGACCTG
AGTAGTTATCCAGAAGCGACAGAAAAAAACGTAGAAAGGATTTTGGGATTGTTGCAAACCTACTTTCGAA
AGTATCCTGATACTCCTGTGTCCTATTTTGAGTTTGTGATTGATCCAAACTCTTTTTCTCGTACTGTGGA
GAATATATTTTATGTTTCTTTTATTGTAAGAGATGGTTTTGCAAGAATAAGGCTTGATGAAGACAGGCTG
CCAATATTAGAGCCGATGAATGTTAACCAAATGGGTGAGGGAAATGATTCCAGTTGCCATGGCAGGAAAC
AGGGAGTTATATCTTTGACTTTACAGGAGTGGAAAAACATTGTGGCAGCTTTTGAAATTTCTGAGGCTAT
GATTACATACTCCTCATAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC205575 protein sequence
Red=Cloning site Green=Tags(s)

MKMDVSVRAAGCSDDLSSGEADVDPKLLELTADEEKCRSIRRQYRQLMYCVRQNREDIVSSANNSLTEAL
EEANVLFDGVSRTREAALDARFLVMASDLGKEKAKQLNSDMNFFNQLAFCDFLFLFVGLNWMEGDPDKLS
DCDDSIALSFWKAIEKEATSWMVKAETFHFVFGSFKLERSAPKPRLEHQKKVRKMEENGNMPTKLQKLDL
SSYPEATEKNVERILGLLQTYFRKYPDTPVSYFEFVIDPNSFSRTVENIFYVSFIVRDGFARIRLDEDRL
PILEPMNVNQMGEGNDSSCHGRKQGVISLTLQEWKNIVAAFEISEAMITYSSY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001008394
ORF Size 999 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001008394.3
RefSeq Size 1486 bp
RefSeq ORF 1002 bp
Locus ID 493861
UniProt ID Q8N140, A0A140VJI9
Cytogenetics 12q23.3
MW 38.2 kDa
Summary Tissue-specific component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination and mediates sumoylation of shelterin complex (telosome) components.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001008394" in other vectors (4)
SKU Description Size Price
RC205575L3 Lenti ORF clone of Human EP300 interacting inhibitor of differentiation 3 (EID3), Myc-DDK-tagged 10 ug
$600.00
RC205575L4 Lenti ORF clone of Human EP300 interacting inhibitor of differentiation 3 (EID3), mGFP tagged 10 ug
$600.00
RG205575 EID3 (tGFP-tagged) - Human EP300 interacting inhibitor of differentiation 3 (EID3) 10 ug
$489.00 $500.00
SC301286 EID3 (untagged)-Human EP300 interacting inhibitor of differentiation 3 (EID3) 10 ug
$457.00

Plasmid Map for RC205575

Download sequence for

Cloning scheme for RC205575

Circular map for RC205575

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