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LRRN5 (LRRN2) (NM_201630) Human Tagged ORF Clone

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SKU
RC205493
LRRN2 (Myc-DDK-tagged)-Human leucine rich repeat neuronal 2 (LRRN2), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $653.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LRRN5
Synonyms FIGLER7; GAC1; LRANK1; LRRN5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205493 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGCTTCTCGTGGCCCCACTCTTGCTAGCTTGGGTGGCTGGTGCCACTGCCGCTGTGCCCGTGGTAC
CCTGGCATGTTCCCTGCCCCCCTCAGTGTGCCTGCCAGATCCGGCCCTGGTATACGCCCCGCTCGTCCTA
CCGCGAGGCTACCACTGTGGACTGCAATGACCTATTCCTGACGGCAGTCCCCCCGGCACTCCCCGCAGGC
ACACAGACCCTGCTCCTGCAGAGCAACAGCATTGTCCGTGTGGACCAGAGTGAGCTGGGCTACCTGGCCA
ATCTCACAGAGCTGGACCTGTCCCAGAACAGCTTTTCGGATGCCCGAGACTGTGATTTCCATGCCCTGCC
CCAGCTGCTGAGCCTGCACCTAGAGGAGAACCAGCTGACCCGGCTGGAGGACCACAGCTTTGCAGGGCTG
GCCAGCCTACAGGAACTCTATCTCAACCACAACCAGCTCTACCGCATCGCCCCCAGGGCCTTTTCTGGCC
TCAGCAACTTGCTGCGGCTGCACCTCAACTCCAACCTCCTGAGGGCCATTGACAGCCGCTGGTTTGAAAT
GCTGCCCAACTTGGAGATACTCATGATTGGCGGCAACAAGGTAGATGCCATCCTGGACATGAACTTCCGG
CCCCTGGCCAACCTGCGTAGCCTGGTGCTAGCAGGCATGAACCTGCGGGAGATCTCCGACTATGCCCTGG
AGGGGCTGCAAAGCCTGGAGAGCCTCTCCTTCTATGACAACCAGCTGGCCCGGGTGCCCAGGCGGGCACT
GGAACAGGTGCCCGGGCTCAAGTTCCTAGACCTCAACAAGAACCCGCTCCAGCGGGTAGGGCCGGGGGAC
TTTGCCAACATGCTGCACCTTAAGGAGCTGGGACTGAACAACATGGAGGAGCTGGTCTCCATCGACAAGT
TTGCCCTGGTGAACCTCCCCGAGCTGACCAAGCTGGACATCACCAATAACCCACGGCTGTCCTTCATCCA
CCCCCGCGCCTTCCACCACCTGCCCCAGATGGAGACCCTCATGCTCAACAACAACGCTCTCAGTGCCTTG
CACCAGCAGACGGCGGAGTCCCTGCCCAACCTGCAGGAGGTAGGTCTCCACGGCAACCCCATCCGCTGTG
ACTGTGTCATCCGCTGGGCCAATGCCACGGGCACCCGTGTCCGCTTCATCGAGCCGCAATCCACCCTGTG
TGCGGAGCCTCCGGACCTCCAGCGCCTCCCGGTCCGTGAGGTGCCCTTCCGGGAGATGACGGACCACTGT
TTGCCCCTCATCTCCCCACGAAGCTTCCCCCCAAGCCTCCAGGTAGCCAGTGGAGAGAGCATGGTGCTGC
ATTGCCGGGCACTGGCCGAACCCGAACCCGAGATCTACTGGGTCACTCCAGCTGGGCTTCGACTGACACC
TGCCCATGCAGGCAGGAGGTACCGGGTGTACCCCGAGGGGACCCTGGAGCTGCGGAGGGTGACAGCAGAA
GAGGCGGGGCTATACACCTGTGTGGCCCAGAACCTGGTGGGGGCTGACACTAAGACGGTTAGTGTGGTTG
TGGGCCGTGCTCTCCTCCAGCCAGGCAGGGACGAAGGACAGGGGCTGGAGCTCCGGGTGCAGGAGACCCA
CCCCTATCACATCCTGCTATCTTGGGTCACCCCACCCAACACAGTGTCCACCAACCTCACCTGGTCCAGT
GCCTCCTCCCTCCGGGGCCAGGGGGCCACAGCTCTGGCCCGCCTGCCTCGGGGAACCCACAGCTACAACA
TTACCCGCCTCCTTCAGGCCACGGAGTACTGGGCCTGCCTGCAAGTGGCCTTTGCTGATGCCCACACCCA
GTTGGCTTGTGTATGGGCCAGGACCAAAGAGGCCACTTCTTGCCACAGAGCCTTAGGGGACCGTCCTGGG
CTCATTGCCATCCTGGCTCTCGCTGTCCTTCTCCTGGCAGCTGGGCTAGCGGCCCACCTTGGCACAGGCC
AACCCAGGAAGGGTGTGGGTGGGAGGCGGCCTCTCCCTCCAGCCTGGGCTTTCTGGGGCTGGAGTCCCCC
TTCTGTCCGGGTTGTGTCTGCTCCCCTCGTCCTGCCCTGGAATCCAGGGAGGAAGCTGCCCAGATCCTCA
GAAGGGGAGACACTGTTGCCACCATTGTCTCAAAATTCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205493 protein sequence
Red=Cloning site Green=Tags(s)

MRLLVAPLLLAWVAGATAAVPVVPWHVPCPPQCACQIRPWYTPRSSYREATTVDCNDLFLTAVPPALPAG
TQTLLLQSNSIVRVDQSELGYLANLTELDLSQNSFSDARDCDFHALPQLLSLHLEENQLTRLEDHSFAGL
ASLQELYLNHNQLYRIAPRAFSGLSNLLRLHLNSNLLRAIDSRWFEMLPNLEILMIGGNKVDAILDMNFR
PLANLRSLVLAGMNLREISDYALEGLQSLESLSFYDNQLARVPRRALEQVPGLKFLDLNKNPLQRVGPGD
FANMLHLKELGLNNMEELVSIDKFALVNLPELTKLDITNNPRLSFIHPRAFHHLPQMETLMLNNNALSAL
HQQTAESLPNLQEVGLHGNPIRCDCVIRWANATGTRVRFIEPQSTLCAEPPDLQRLPVREVPFREMTDHC
LPLISPRSFPPSLQVASGESMVLHCRALAEPEPEIYWVTPAGLRLTPAHAGRRYRVYPEGTLELRRVTAE
EAGLYTCVAQNLVGADTKTVSVVVGRALLQPGRDEGQGLELRVQETHPYHILLSWVTPPNTVSTNLTWSS
ASSLRGQGATALARLPRGTHSYNITRLLQATEYWACLQVAFADAHTQLACVWARTKEATSCHRALGDRPG
LIAILALAVLLLAAGLAAHLGTGQPRKGVGGRRPLPPAWAFWGWSPPSVRVVSAPLVLPWNPGRKLPRSS
EGETLLPPLSQNS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_201630
ORF Size 2139 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_201630.1, NP_963924.1
RefSeq Size 3207 bp
RefSeq ORF 2142 bp
Locus ID 10446
UniProt ID O75325, A0A024R993
Cytogenetics 1q32.1
Protein Families Druggable Genome, Transmembrane
MW 78.9 kDa
Summary The protein encoded by this gene belongs to the leucine-rich repeat superfamily. This gene was found to be amplified and overexpressed in malignant gliomas. The encoded protein has homology with other proteins that function as cell-adhesion molecules or as signal transduction receptors and is a candidate for the target gene in the 1q32.1 amplicon in malignant gliomas. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. provided by RefSeq, Jul 2008
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"NM_201630" in other vectors (4)
SKU Description Size Price
RC205493L3 Lenti ORF clone of Human leucine rich repeat neuronal 2 (LRRN2), transcript variant 2, Myc-DDK-tagged 10 ug
$953.00
RC205493L4 Lenti ORF clone of Human leucine rich repeat neuronal 2 (LRRN2), transcript variant 2, mGFP tagged 10 ug
$953.00
RG205493 LRRN2 (tGFP-tagged) - Human leucine rich repeat neuronal 2 (LRRN2), transcript variant 2 10 ug
$789.00 $853.00
SC308077 LRRN2 (untagged)-Human leucine rich repeat neuronal 2 (LRRN2), transcript variant 2 10 ug
$719.00

Plasmid Map for RC205493

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205493
Circular map for RC205493

file-alt Citations

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