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PIWIL2 (NM_018068) Human Tagged ORF Clone

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SKU
RC205464
PIWIL2 (Myc-DDK-tagged)-Human piwi-like 2 (Drosophila) (PIWIL2), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $891.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PIWIL2
Synonyms CT80; HILI; mili; PIWIL1L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205464 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCCTTTCCGACCATCGTTCAGGGGCCAGTCTCCTATCCACCCATCCCAGTGCCAGGCTGTACGGA
TGCCAGGCTGTTGGCCACAAGCTTCTAAACCTTTGGACCCAGCTCTGGGCAGGGGAGCACCTGCAGGCAG
AGGCCATGTATTTGGAAAGCCAGAGGAACCAAGCACACAGAGGGGGCCAGCACAAAGGGAGTCTGTGGGT
TTGGTCTCCATGTTCCGAGGCCTGGGCATTGAAACAGTTTCTAAGACCCCTCTGAAACGGGAAATGCTTC
CATCAGGTAGAGGCATTTTAGGTCGAGGCTTGTCTGCTAATCTGGTACGCAAGGACAGGGAGGAACTCTC
TCCCACTTTTTGGGATCCAAAAGTGTTGGCGGCTGGGGACAGCAAGATGGCAGAGACCTCCGTTGGTTGG
AGTAGGACGCTTGGAAGAGGGAGTTCAGATGCGTCTTTATTACCACTGGGAAGAGCAGCAGGTGGTATCA
GCAGAGAAGTGGACAAGCCTCCCTGTACCTTCAGCACACCGTCCCGGGGTCCCCCGCAGCTGTCATCACC
ACCAGCTCTGCCCCAGTCTCCCCTGCACTCTCCAGATCGCCCTCTGGTCCTGACTGTGGAACACAAGGAA
AAAGAGCTTATTGTGAAGCAAGGATCAAAAGGAACACCTCAGTCTTTGGGACTGAACCTCGTCAAAATAC
AGTGTCATAATGAAGCAGTTTATCAATATCATGTGACTTTCAGCCCCAATGTGGAGTGCAAAAGCATGAG
GTTCGGCATGTTGAAGGACCATCAAGCTGTCACCGGCAACGTCACTGCGTTTGATGGATCTATTCTCTAT
CTGCCTGTTAAGCTTCAACAAGTTCTTGAGTTAAAAAGTCAAAGGAAAACAGACAGTGCTGAAATCAGCA
TTAAGATTCAGATGACAAAGATCCTGGAGCCCTGCTCTGACCTGTGCATTCCCTTCTACAATGTTGTTTT
CCGTCGGGTAATGAAACTTTTAGATATGAAGCTTGTGGGGAGAAACTTTTATGACCCTACAAGTGCTATG
GTACTACAGCAACACAGATTGCAGATCTGGCCAGGCTATGCAGCTAGCATCCGAAGGACAGATGGAGGGC
TCTTCCTGCTAGCTGATGTCTCCCATAAGGTCATTCGGAATGACTGTGTGCTGGATGTCATGCATGCCAT
TTATCAGCAGAATAAAGAACACTTCCAGGATGAGTGTACTAAGCTTCTGGTTGGCAATATTGTTATCACC
CGATATAACAATCGTACCTATCGTATTGATGATGTGGATTGGAATAAGACTCCAAAGGATAGCTTCACGA
TGTCTGATGGGAAAGAGATCACATTCTTGGAATACTACAGCAAAAATTATGGGATCACAGTTAAGGAAGA
GGACCAGCCATTGCTGATTCACAGGCCCAGTGAGAGACAGGATAATCATGGGATGCTGCTAAAAGGGGAA
ATCCTGCTGCTGCCTGAGCTTTCTTTTATGACCGGAATCCCAGAGAAGATGAAGAAGGACTTCAGAGCCA
TGAAGGATTTGGCTCAGCAAATCAATCTGAGCCCCAAGCAACACCATAGTGCTTTGGAATGCTTGCTGCA
AAGAATTGCAAAGAACGAGGCAGCCACCAATGAACTGATGCGTTGGGGGCTCCGTCTGCAAAAGGATGTA
CATAAGATTGAAGGACGTGTTCTGCCAATGGAAAGAATTAACTTAAAAAATACTTCGTTTATCACATCTC
AGGAACTAAACTGGGTTAAGGAAGTAACCAGAGACCCTTCCATCTTGACTATCCCCATGCATTTCTGGGC
ACTTTTTTACCCAAAGAGAGCAATGGACCAGGCTCGAGAACTGGTCAACATGTTGGAGAAGATAGCCGGC
CCCATTGGCATGCGTATGAGCCCACCGGCCTGGGTTGAACTAAAGGATGACCGAATAGAGACTTATGTCA
GAACCATTCAATCCACGTTAGGAGCTGAGGGGAAGATACAGATGGTTGTTTGCATCATCATGGGCCCACG
TGATGATCTCTATGGGGCCATCAAGAAGCTGTGCTGTGTGCAGTCCCCAGTGCCCTCCCAGGTTGTCAAT
GTTCGAACCATTGGTCAGCCCACCAGGCTTCGGAGTGTGGCCCAGAAGATTTTACTTCAGATTAACTGTA
AATTGGGTGGTGAGCTCTGGGGAGTGGATATTCCTCTGAAACAGTTAATGGTGATCGGGATGGATGTTTA
CCATGACCCCAGTAGAGGCATGCGCTCCGTGGTTGGCTTCGTGGCAAGCATCAATCTCACCCTCACAAAA
TGGTATTCCCGGGTGGTGTTCCAGATGCCGCATCAGGAGATTGTGGACAGCCTGAAGCTATGCCTCGTGG
GCTCCTTAAAAAAGTTTTATGAGGTGAACCACTGTCTACCAGAGAAGATTGTGGTGTACCGTGATGGAGT
GTCTGATGGCCAACTGAAGACAGTTGCCAACTATGAGATTCCTCAACTACAGAAGTGTTTTGAAGCTTTT
GAGAATTATCAGCCCAAGATGGTGGTGTTTGTAGTTCAGAAGAAAATCAGTACTAATCTATATCTGGCTG
CTCCTCAGAACTTTGTAACTCCCACTCCTGGAACTGTGGTAGATCATACAATAACAAGCTGTGAGTGGGT
GGATTTCTATCTTCTTGCCCATCATGTACGGCAGGGCTGTGGCATTCCTACGCATTATGTCTGTGTTCTC
AACACCGCAAACCTGAGCCCTGATCATATGCAGAGGCTGACTTTCAAACTGTGCCACATGTACTGGAATT
GGCCTGGCACCATCAGAGTTCCAGCTCCTTGCAAGTATGCCCACAAGCTAGCTTTCCTGTCAGGACACAT
CTTGCATCATGAACCAGCCATCCAGCTGTGCGAGAACCTGTTCTTCCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205464 protein sequence
Red=Cloning site Green=Tags(s)

MDPFRPSFRGQSPIHPSQCQAVRMPGCWPQASKPLDPALGRGAPAGRGHVFGKPEEPSTQRGPAQRESVG
LVSMFRGLGIETVSKTPLKREMLPSGRGILGRGLSANLVRKDREELSPTFWDPKVLAAGDSKMAETSVGW
SRTLGRGSSDASLLPLGRAAGGISREVDKPPCTFSTPSRGPPQLSSPPALPQSPLHSPDRPLVLTVEHKE
KELIVKQGSKGTPQSLGLNLVKIQCHNEAVYQYHVTFSPNVECKSMRFGMLKDHQAVTGNVTAFDGSILY
LPVKLQQVLELKSQRKTDSAEISIKIQMTKILEPCSDLCIPFYNVVFRRVMKLLDMKLVGRNFYDPTSAM
VLQQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDCVLDVMHAIYQQNKEHFQDECTKLLVGNIVIT
RYNNRTYRIDDVDWNKTPKDSFTMSDGKEITFLEYYSKNYGITVKEEDQPLLIHRPSERQDNHGMLLKGE
ILLLPELSFMTGIPEKMKKDFRAMKDLAQQINLSPKQHHSALECLLQRIAKNEAATNELMRWGLRLQKDV
HKIEGRVLPMERINLKNTSFITSQELNWVKEVTRDPSILTIPMHFWALFYPKRAMDQARELVNMLEKIAG
PIGMRMSPPAWVELKDDRIETYVRTIQSTLGAEGKIQMVVCIIMGPRDDLYGAIKKLCCVQSPVPSQVVN
VRTIGQPTRLRSVAQKILLQINCKLGGELWGVDIPLKQLMVIGMDVYHDPSRGMRSVVGFVASINLTLTK
WYSRVVFQMPHQEIVDSLKLCLVGSLKKFYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAF
ENYQPKMVVFVVQKKISTNLYLAAPQNFVTPTPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYVCVL
NTANLSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGHILHHEPAIQLCENLFFL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018068
ORF Size 2919 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018068.5
RefSeq Size 3636 bp
RefSeq ORF 2922 bp
Locus ID 55124
UniProt ID Q8TC59, W0HK13
Cytogenetics 8p21.3
Domains PAZ, Piwi
Protein Pathways Dorso-ventral axis formation
MW 109.8 kDa
Summary PIWIL2 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 PubMed 12906857).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_018068" in other vectors (6)
SKU Description Size Price
RC205464L1 Lenti ORF clone of Human piwi-like 2 (Drosophila) (PIWIL2), transcript variant 2, Myc-DDK-tagged 10 ug
$1,191.00
RC205464L2 Lenti ORF clone of Human piwi-like 2 (Drosophila) (PIWIL2), transcript variant 2, mGFP tagged 10 ug
$1,191.00
RC205464L3 Lenti ORF clone of Human piwi-like 2 (Drosophila) (PIWIL2), transcript variant 2, Myc-DDK-tagged 10 ug
$1,191.00
RC205464L4 Lenti ORF clone of Human piwi-like 2 (Drosophila) (PIWIL2), transcript variant 2, mGFP tagged 10 ug
$1,191.00
RG205464 PIWIL2 (tGFP-tagged) - Human piwi-like 2 (Drosophila) (PIWIL2), transcript variant 2 10 ug
$789.00 $1,091.00
SC125782 PIWIL2 (untagged)-Human piwi-like 2 (Drosophila) (PIWIL2), transcript variant 2 10 ug
$892.00

Plasmid Map for RC205464

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205464
Circular map for RC205464

file-alt Citations

Dysregulation of a family of short noncoding RNAs, tsRNAs, in human cancer
Pekarsky, Balatti, Palamarchuk et al
PNAS (2016)
Cited by 265
AI Summary: OriGene products contributed to this paper by providing the pCMV6-Entry-mycDDK tagged TrueORF gold expression vectors for PiwiL2 (SKU: RC205464), Ago1 (SKU: RC209163), and Ago2 (SKU: RC218078), which were used to generate constructs for studying protein interactions with tsRNAs in cancer research.
Snippet: For pMAX-piwiL2, pMAX-ago1, and pMAX-ago2, the ORFs of PiwiL2, Ago1, and Ago2 were subcloned in pcDNA4/HisMax (Invitrogen) vector from pCMV6-Entry-mycDDK tagged TrueORF gold expression vector purchased from OriGene [stock-keeping unit (SKU) nos. RC205464, RC209163, andRC218078, respectively]
Showing 1-1 of 1 papers.
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