ACTRT1 (NM_138289) Human Tagged ORF Clone

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SKU
RC205402
ACTRT1 (Myc-DDK-tagged)-Human actin-related protein T1 (ACTRT1)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ACTRT1
Synonyms AIP1; ARIP1; ARPT1; HSD27
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205402 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTAATCCACATGCATTAGATGTTCCTGCTGTAATTTTTGACAATGGTTCAGGACTCTGCAAAGCAG
GCCTGTCTGGAGAGATTGGACCCCGCCATGTCATCAGCTCCGTCTTGGGACATTGTAAATTCAATGTGCC
TTTAGCAAGACTTAATCAGAAGTACTTCGTGGGGCAAGAAGCCCTGTACAAGTATGAGGCCCTACATTTG
CACTACCCCATTGAGCGTGGACTGGTAACAGGATGGGATGACATGGAGAAACTCTGGAAACATCTCTTTG
AGCGGGAGCTTGGAGTAAAACCCAGCCAACAGCCTGTACTTATGACCGAGCCCTCTTTGAATCCTAGGGA
AATTCGAGAAAAGCTAGCAGAAATGATGTTTGAGACCTTCAGTGTGCCTGGTTTCTACCTGTCTAATCAT
GCGGTGGCAGCGCTCTATGCCTCTGCCTGTGTCACAGGCCTGGTGGTGGACAGTGGAGATGGGGTCACTT
GCACTGTCCCCATCTTTGAGGGTTACTCCCTGCCTCACGCAGTCACCAAACTCTGTATGGCAGGGAGGGA
CATCACAGAGCACCTCACCCGGCTCCTCTTTGCTAGCGGGTTTAACTTCCCTTGCATACTCAACAAGGCC
GTGGTAAATAACATCAAAGAGAAGTTGTGCTACATCGCCTTGGAGCCAGAGAAAGAGCTACGCAAGAGCC
GGGGAGAGGTCCTGGGAGCATACAGACTGCCAGATGGACATGTCATCCACTTTGGGGATGAGCTGTACCA
AGTGCCCGAGGTTCTTTTTGCACCTGACCAGCTGGGCATCCACAGCCCAGGACTCTCAAAAATGGTCTCC
AGCAGCATCATGAAGTGTGACACTGACATCCAGAATAAACTTTATGCAGACATTGTACTCTCCGGGGGCA
CCACTCTCCTCCCTGGGCTGGAGGAAAGGCTCATGAAGGAAGTGGAACAGCTGGCTTCCAAAGGTACTCC
CATCAAGATCACAGCTTCTCCTGATAGATGCTTCTCTGCATGGATTGGTGCATCCATCATGACCTCTATG
AGCAGTTTCAAGCAGATGTGGGTCACCTCGGCAGACTTCAAGGAGTATGGGACATCTGTGGTTCAAAGAA
GGTGCTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC205402 protein sequence
Red=Cloning site Green=Tags(s)

MFNPHALDVPAVIFDNGSGLCKAGLSGEIGPRHVISSVLGHCKFNVPLARLNQKYFVGQEALYKYEALHL
HYPIERGLVTGWDDMEKLWKHLFERELGVKPSQQPVLMTEPSLNPREIREKLAEMMFETFSVPGFYLSNH
AVAALYASACVTGLVVDSGDGVTCTVPIFEGYSLPHAVTKLCMAGRDITEHLTRLLFASGFNFPCILNKA
VVNNIKEKLCYIALEPEKELRKSRGEVLGAYRLPDGHVIHFGDELYQVPEVLFAPDQLGIHSPGLSKMVS
SSIMKCDTDIQNKLYADIVLSGGTTLLPGLEERLMKEVEQLASKGTPIKITASPDRCFSAWIGASIMTSM
SSFKQMWVTSADFKEYGTSVVQRRCF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138289
ORF Size 1128 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138289.4
RefSeq Size 1452 bp
RefSeq ORF 1131 bp
Locus ID 139741
UniProt ID Q8TDG2
Cytogenetics Xq25
MW 41.7 kDa
Summary This gene encodes a protein related to the cytoskeletal protein beta-actin. This protein is a major component of the calyx in the perinuclear theca of mammalian sperm heads, and it therefore likely functions in spermatid formation. This gene is intronless and is similar to a related gene located on chromosome 1. A related pseudogene has also been identified approximately 75 kb downstream of this gene on chromosome X. provided by RefSeq, May 2010
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Documents
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"NM_138289" in other vectors (6)
SKU Description Size Price
RC205402L1 Lenti ORF clone of Human actin-related protein T1 (ACTRT1), Myc-DDK-tagged 10 ug
$986.00
RC205402L2 Lenti ORF clone of Human actin-related protein T1 (ACTRT1), mGFP tagged 10 ug
$986.00
RC205402L3 Lenti ORF clone of Human actin-related protein T1 (ACTRT1), Myc-DDK-tagged 10 ug
$986.00
RC205402L4 Lenti ORF clone of Human actin-related protein T1 (ACTRT1), mGFP tagged 10 ug
$986.00
RG205402 ACTRT1 (tGFP-tagged) - Human actin-related protein T1 (ACTRT1) 10 ug
$886.00
SC123127 ACTRT1 (untagged)-Human actin-related protein T1 (ACTRT1) 10 ug
$686.00

Plasmid Map for RC205402

Download sequence for

Cloning scheme for RC205402

Circular map for RC205402

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