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ADH1B (NM_000668) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC205391L3
Lenti ORF clone of Human alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B), Myc-DDK-tagged
ORF Plasmid:
DDK DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $986.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ADH1B
Synonyms ADH2; HEL-S-117
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC205391).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000668
ORF Size 1125 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000668.3, BC033009.2
RefSeq Size 2707 bp
RefSeq ORF 1128 bp
Locus ID 125
UniProt ID P00325
Cytogenetics 4q23
Domains ADH_zinc_N
Protein Families Druggable Genome
Protein Pathways Drug metabolism - cytochrome P450, Fatty acid metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism, Tyrosine metabolism
MW 39.9 kDa
Summary The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Nov 2013
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"NM_000668" in other vectors (6)
SKU Description Size Price
RC205391 ADH1B (Myc-DDK-tagged)-Human alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B) 10 ug
$686.00
RC205391L1 Lenti ORF clone of Human alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B), Myc-DDK-tagged 10 ug
$986.00
RC205391L2 Lenti ORF clone of Human alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B), mGFP tagged 10 ug
$986.00
RC205391L4 Lenti ORF clone of Human alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B), mGFP tagged 10 ug
$986.00
RG205391 ADH1B (tGFP-tagged) - Human alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B) 10 ug
$886.00
SC119751 ADH1B (untagged)-Human alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B) 10 ug
$686.00

Plasmid Map for RC205391L3

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205391L3
Circular map for RC205391L3

file-alt Citations

ADH1B, the adipocyte-enriched alcohol dehydrogenase, plays an essential, cell-autonomous role in human adipogenesis
Gautheron, Elsayed, Pistorio et al
Proc Natl Acad Sci U S A (2024) 121 (24), e2319301121 DOI: 10.1073/pnas.2319301121
Cited by 17
AI Summary: In this study, OriGene products pCMV6-entry-GFP and pCMV-ADH1B WT-Flag were used as plasmid constructs to investigate the function of ADH1B in human adipocyte differentiation.
Snippet: ty Kit Thermo Fisher Scientific Cat# TR22421 TurboFect Thermo Fisher Scientific Cat# R0532 Recombinant DNA lentiCRISPR v2 Addgene Cat# 52961 pCMV6-entry-GFP Origene Cat# PS100026 pCMV-ADH1B WT-Flag Origene Cat# RC205391L3 pCMV-ADH1B c.937C>T-Flag Described in Methods N/A Software and Algorithms Multi-Experiment Viewer (MeV)
FigureFigureFigure
Further evidence supporting a potential role for ADH1B in obesity
Morales, Cromack, Tripathy et al
Sci Rep (2021) 11 (1), 1932 DOI: 10.1038/s41598-020-80563-z
Cited by 27
AI Summary: OriGene's anti-ADH1A antibody was used in this study to detect ADH1A protein levels, contributing to the analysis of alcohol dehydrogenase family members in relation to obesity and insulin resistance.
Snippet: pore (St. Louis, MO) and diluted in medium as needed. Anti-ADH1B, anti-ADH1C, anti-FABP4, and anti-adiponectin antibodies were purchased from Abcam Inc. (Cambridge, MA). Anti-ADH1A was purchased from Origene Technologies, Inc. (Rockville, MD). Anti-DDK, anti-Akt, anti-Phospho-Akt, and anti-PPARγ were purchased from Cell Signaling (Danvers, MA). Anti-GLUT4 was purchased form Bioss, Inc (Boston, MA
FigureFigureFigure
Showing 1-2 of 2 papers.
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