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DPP8 (NM_197961) Human Tagged ORF Clone

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SKU
RC205236
DPP8 (Myc-DDK-tagged)-Human dipeptidyl-peptidase 8 (DPP8), transcript variant 4
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $776.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DPP8
Synonyms DP8; DPRP-1; DPRP1; MST097; MSTP097; MSTP135; MSTP141
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205236 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGAAGAGATCTGAGCAGATGAAAATAAAATCAGGAAAATGCAACATGGCAGCAGCAATGGAAACAG
AACAGCTGGGTGTTGAGATATTTGAAACTGCGGACTGTGAGGAGAATATTGAATCACAGGATCGGCCTAA
ATTGGAGCCTTTTTATGTTGAGCGGTATTCCTGGAGTCAGCTTAAAAAGCTGCTTGCCGATACCAGAAAA
TATCATGGCTACATGATGGCTAAGGCACCACATGATTTCATGTTTGTGAAGAGGAATGATCCAGATGGAC
CTCATTCAGACAGAATCTATTACCTTGCCATGTCTGGTGAGAACAGAGAAAATACACTGTTTTATTCTGA
AATTCCCAAAACTATCAATAGAGCAGCAGTCTTAATGCTCTCTTGGAAGCCTCTTTTGGATCTTTTTCAG
GCAACACTGGACTATGGAATGTATTCTCGAGAAGAAGAACTATTAAGAGAAAGAAAACGCATTGGAACAG
TCGGAATTGCTTCTTACGATTATCACCAAGGAAGTGGAACATTTCTGTTTCAAGCCGGTAGTGGAATTTA
TCACGTAAAAGATGGAGGGCCACAAGGATTTACGCAACAACCTTTAAGGCCCAATCTAGTGGAAACTAGT
TGTCCCAACATACGGATGGATCCAAAATTATGCCCTGCTGATCCAGACTGGATTGCTTTTATACATAGCA
ACGATATTTGGATATCTAACATCGTAACCAGAGAAGAAAGGAGACTCACTTATGTGCACAATGAGCTAGC
CAACATGGAAGAAGATGCCAGATCAGCTGGAGTCGCTACCTTTGTTCTCCAAGAAGAATTTGATAGATAT
TCTGGCTATTGGTGGTGTCCAAAAGCTGAAACAACTCCCAGTGGTGGTAAAATTCTTAGAATTCTATATG
AAGAAAATGATGAATCTGAGGTGGAAATTATTCATGTTACATCCCCTATGTTGGAAACAAGGAGGGCAGA
TTCATTCCGTTATCCTAAAACAGGTACAGCAAATCCTAAAGTCACTTTTAAGATGTCAGAAATAATGATT
GATGCTGAAGGAAGGATCATAGATGTCATAGATAAGGAACTAATTCAACCTTTTGAGATTCTATTTGAAG
GAGTTGAATATATTGCCAGAGCTGGATGGACTCCTGAGGGAAAATATGCTTGGTCCATCCTACTAGATCG
CTCCCAGACTCGCCTACAGATAGTGTTGATCTCACCTGAATTATTTATCCCAGTAGAAGATGATGTTATG
GAAAGGCAGAGACTCATTGAGTCAGTGCCTGATTCTGTGACGCCACTAATTATCTATGAAGAAACAACAG
ACATCTGGATAAATATCCATGACATCTTTCATGTTTTTCCCCAAAGTCACGAAGAGGAAATTGAGTTTAT
TTTTGCCTCTGAATGCAAAACAGGTTTCCGTCATTTATACAAAATTACATCTATTTTAAAGGAAAGCAAA
TATAAACGATCCAGTGGTGGGCTGCCTGCTCCAAGTGATTTCAAGTGTCCTATCAAAGAGGAGATAGCAA
TTACCAGTGGTGAATGGGAAGTTCTTGGCCGGCATGGATCTAATATCCAAGTTGATGAAGTCAGAAGGCT
GGTATATTTTGAAGGCACCAAAGACTCCCCTTTAGAGCATCACCTGTACGTAGTCAGTTACGTAAATCCT
GGAGAGGTGACAAGGCTGACTGACCGTGGCTACTCACATTCTTGCTGCATCAGTCAGCACTGTGACTTCT
TTATAAGTAAGTATAGTAACCAGAAGAATCCACACTGTGTGTCCCTTTACAAGCTATCAAGTCCTGAAGA
TGACCCAACTTGCAAAACAAAGGAATTTTGGGCCACCATTTTGGATTCAGCAGGTCCTCTTCCTGACTAT
ACTCCTCCAGAAATTTTCTCTTTTGAAAGTACTACTGGATTTACATTGTATGGGATGCTCTACAAGCCTC
ATGATCTACAGCCTGGAAAGAAATATCCTACTGTGCTGTTCATATATGGTGGTCCTCAGGTGCAGTTGGT
GAATAATCGGTTTAAAGGAGTCAAGTATTTCCGCTTGAATACCCTAGCCTCTCTAGGTTATGTGGTTGTA
GTGATAGACAACAGGGGATCCTGTCACCGAGGGCTTAAATTTGAAGGCGCCTTTAAATATAAAATGGTTG
CTATTGCTGGGGCCCCAGTCACTCTGTGGATCTTCTATGATACAGGATACACGGAACGTTATATGGGTCA
CCCTGACCAGAATGAACAGGGCTATTACTTAGGATCTGTGGCCATGCAAGCAGAAAAGTTCCCCTCTGAA
CCAAATCGTTTACTGCTCTTACATGGTTTCCTGGATGAGAATGTCCATTTTGCACATACCAGTATATTAC
TGAGTTTTTTAGTGAGGGCTGGAAAGCCATATGATTTACAGATCTATCCTCAGGAGAGACACAGCATAAG
AGTTCCTGAATCGGGAGAACATTATGAACTGCATCTTTTGCACTACCTTCAAGAAAACCTTGGATCACGT
ATTGCTGCTCTAAAAGTGATA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205236 protein sequence
Red=Cloning site Green=Tags(s)

MWKRSEQMKIKSGKCNMAAAMETEQLGVEIFETADCEENIESQDRPKLEPFYVERYSWSQLKKLLADTRK
YHGYMMAKAPHDFMFVKRNDPDGPHSDRIYYLAMSGENRENTLFYSEIPKTINRAAVLMLSWKPLLDLFQ
ATLDYGMYSREEELLRERKRIGTVGIASYDYHQGSGTFLFQAGSGIYHVKDGGPQGFTQQPLRPNLVETS
CPNIRMDPKLCPADPDWIAFIHSNDIWISNIVTREERRLTYVHNELANMEEDARSAGVATFVLQEEFDRY
SGYWWCPKAETTPSGGKILRILYEENDESEVEIIHVTSPMLETRRADSFRYPKTGTANPKVTFKMSEIMI
DAEGRIIDVIDKELIQPFEILFEGVEYIARAGWTPEGKYAWSILLDRSQTRLQIVLISPELFIPVEDDVM
ERQRLIESVPDSVTPLIIYEETTDIWINIHDIFHVFPQSHEEEIEFIFASECKTGFRHLYKITSILKESK
YKRSSGGLPAPSDFKCPIKEEIAITSGEWEVLGRHGSNIQVDEVRRLVYFEGTKDSPLEHHLYVVSYVNP
GEVTRLTDRGYSHSCCISQHCDFFISKYSNQKNPHCVSLYKLSSPEDDPTCKTKEFWATILDSAGPLPDY
TPPEIFSFESTTGFTLYGMLYKPHDLQPGKKYPTVLFIYGGPQVQLVNNRFKGVKYFRLNTLASLGYVVV
VIDNRGSCHRGLKFEGAFKYKMVAIAGAPVTLWIFYDTGYTERYMGHPDQNEQGYYLGSVAMQAEKFPSE
PNRLLLLHGFLDENVHFAHTSILLSFLVRAGKPYDLQIYPQERHSIRVPESGEHYELHLLHYLQENLGSR
IAALKVI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_197961
ORF Size 2541 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_197961.4
RefSeq Size 4079 bp
RefSeq ORF 2544 bp
Locus ID 54878
UniProt ID Q6V1X1
Cytogenetics 15q22.31
Protein Families Druggable Genome, Protease, Transmembrane
MW 97.5 kDa
Summary This gene encodes a member of the peptidase S9B family, a small family of dipeptidyl peptidases that are able to cleave peptide substrates at a prolyl bond. The encoded protein shares similarity with dipeptidyl peptidase IV in that it is ubiquitously expressed, and hydrolyzes the same substrates. These similarities suggest that, like dipeptidyl peptidase IV, this protein may play a role in T-cell activation and immune function. Alternatively spliced transcript variants encoding different isoforms have been described. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_197961" in other vectors (6)
SKU Description Size Price
RC205236L1 Lenti ORF clone of Human dipeptidyl-peptidase 8 (DPP8), transcript variant 4, Myc-DDK-tagged 10 ug
$1,076.00
RC205236L2 Lenti ORF clone of Human dipeptidyl-peptidase 8 (DPP8), transcript variant 4, mGFP tagged 10 ug
$1,076.00
RC205236L3 Lenti ORF clone of Human dipeptidyl-peptidase 8 (DPP8), transcript variant 4, Myc-DDK-tagged 10 ug
$1,076.00
RC205236L4 Lenti ORF clone of Human dipeptidyl-peptidase 8 (DPP8), transcript variant 4, mGFP tagged 10 ug
$1,076.00
RG205236 DPP8 (tGFP-tagged) - Human dipeptidyl-peptidase 8 (DPP8), transcript variant 4 10 ug
$789.00 $976.00
SC110497 DPP8 (untagged)-Human dipeptidyl-peptidase 8 (DPP8), transcript variant 4 10 ug
$776.00

Plasmid Map for RC205236

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205236
Circular map for RC205236

file-alt Citations

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