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PHAPI2 / APRIL (ANP32B) (NM_006401) Human Tagged ORF Clone

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SKU
RC205158
ANP32B (Myc-DDK-tagged)-Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member B (ANP32B)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PHAPI2 / APRIL
Synonyms APRIL; PHAPI2; SSP29
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205158 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACATGAAGAGGAGGATCCACCTGGAGCTGAGGAACCGGACCCCGGCAGCTGTTCGAGAACTTGTCT
TGGACAATTGCAAATCAAATGATGGAAAAATTGAGGGCTTAACAGCTGAATTTGTGAACTTAGAGTTCCT
CAGTTTAATAAATGTAGGCTTGATCTCAGTTTCAAATCTCCCCAAGCTGCCTAAATTGAAAAAGCTTGAA
CTCAGTGAAAATAGAATCTTTGGAGGTCTGGACATGTTAGCTGAAAAACTTCCAAATCTCACACATCTAA
ACTTAAGTGGAAATAAACTGAAAGATATCAGCACCTTGGAACCTTTGAAAAAGTTAGAATGTCTGAAAAG
CCTGGACCTCTTTAACTGTGAGGTTACCAACCTGAATGACTACCGAGAGAGTGTCTTCAAGCTCCTGCCC
CAGCTTACCTACTTGGATGGCTATGACCGAGAGGACCAGGAAGCACCTGACTCAGATGCCGAGGTGGATG
GTGTGGATGAAGAGGAGGAGGACGAAGAAGGAGAAGATGAGGAAGACGAGGACGATGAGGATGGTGAAGA
AGAGGAGTTTGATGAAGAAGATGATGAAGATGAAGATGTAGAAGGGGATGAGGACGACGATGAAGTCAGT
GAGGAGGAAGAAGAATTTGGACTTGATGAAGAAGATGAAGATGAGGATGAGGATGAAGAGGAGGAAGAAG
GTGGGAAAGGTGAAAAGAGGAAGAGAGAAACAGATGATGAAGGAGAAGATGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205158 protein sequence
Red=Cloning site Green=Tags(s)

MDMKRRIHLELRNRTPAAVRELVLDNCKSNDGKIEGLTAEFVNLEFLSLINVGLISVSNLPKLPKLKKLE
LSENRIFGGLDMLAEKLPNLTHLNLSGNKLKDISTLEPLKKLECLKSLDLFNCEVTNLNDYRESVFKLLP
QLTYLDGYDREDQEAPDSDAEVDGVDEEEEDEEGEDEEDEDDEDGEEEEFDEEDDEDEDVEGDEDDDEVS
EEEEEFGLDEEDEDEDEDEEEEEGGKGEKRKRETDDEGEDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006401
ORF Size 753 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006401.3
RefSeq Size 1617 bp
RefSeq ORF 756 bp
Locus ID 10541
UniProt ID Q92688
Cytogenetics 9q22.33
Domains LRR, LRRcap
MW 28.8 kDa
Summary Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome.UniProtKB/Swiss-Prot Function
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Documents
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"NM_006401" in other vectors (6)
SKU Description Size Price
RC205158L1 Lenti ORF clone of Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member B (ANP32B), Myc-DDK-tagged 10 ug
$600.00
RC205158L2 Lenti ORF clone of Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member B (ANP32B), mGFP tagged 10 ug
$600.00
RC205158L3 Lenti ORF clone of Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member B (ANP32B), Myc-DDK-tagged 10 ug
$600.00
RC205158L4 Lenti ORF clone of Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member B (ANP32B), mGFP tagged 10 ug
$600.00
RG205158 ANP32B (tGFP-tagged) - Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member B (ANP32B) 10 ug
$489.00 $500.00
SC116090 ANP32B (untagged)-Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member B (ANP32B) 10 ug
$300.00

Plasmid Map for RC205158

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205158
Circular map for RC205158

file-alt Citations

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