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SERPINB8 (NM_001031848) Human Tagged ORF Clone

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SKU
RC205135
SERPINB8 (Myc-DDK-tagged)-Human serpin peptidase inhibitor, clade B (ovalbumin), member 8 (SERPINB8), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SERPINB8
Synonyms C18orf53; CAP2; PI-8; PI8; PSS5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205135 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGACCTCTGTGAAGCAAATGGCACTTTTGCCATCAGCTTATTTAAAATATTGGGGGAAGAGGACA
ACTCAAGAAACGTATTCTTCTCTCCCATGAGCATCTCCTCTGCCCTGGCCATGGTCTTCATGGGGGCAAA
GGGAAGCACTGCAGCCCAGATGTCCCAGGCACTTTGTTTATACAAAGACGGAGATATTCACCGAGGTTTC
CAGTCACTTCTCAGTGAAGTTAACAGAACTGGCACTCAGTACTTGCTTAGAACTGCCAACAGACTCTTTG
GAGAAAAGACGTGTGATTTCCTTCCAGACTTTAAAGAATACTGTCAGAAGTTCTATCAGGCAGAGCTGGA
GGAGTTGTCCTTTGCTGAAGACACTGAAGAGTGCAGGAAGCATATAAATGACTGGGTGGCAGAGAAGACT
GAAGGTAAGATTTCAGAGGTACTGGATGCTGGGACAGTCGATCCCCTGACAAAGCTAGTCCTTGTGAATG
CCATTTATTTCAAGGGAAAGTGGAATGAGCAATTTGACAGAAAGTACACAAGGGGAATGCTCTTTAAAAC
CAACGAGGAAAAAAAGACAGTGCAGATGATGTTTAAGGAAGCTAAGTTTAAAATGGGGTATGCGGATGAG
GTACACACCCAGGTCCTGGAGCTGCCCTATGTGGAAGAGGAGCTGAGCATGGTCATTCTGCTTCCCGATG
ACAACACGGACCTCGCCGTGAAAGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205135 protein sequence
Red=Cloning site Green=Tags(s)

MDDLCEANGTFAISLFKILGEEDNSRNVFFSPMSISSALAMVFMGAKGSTAAQMSQALCLYKDGDIHRGF
QSLLSEVNRTGTQYLLRTANRLFGEKTCDFLPDFKEYCQKFYQAELEELSFAEDTEECRKHINDWVAEKT
EGKISEVLDAGTVDPLTKLVLVNAIYFKGKWNEQFDRKYTRGMLFKTNEEKKTVQMMFKEAKFKMGYADE
VHTQVLELPYVEEELSMVILLPDDNTDLAVKE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001031848
ORF Size 726 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001031848.1, NP_001027018.1
RefSeq Size 1409 bp
RefSeq ORF 729 bp
Locus ID 5271
UniProt ID P50452, Q8N178
Cytogenetics 18q22.1
Protein Families Druggable Genome
MW 27.7 kDa
Summary The protein encoded by this gene is a member of the ov-serpin family of serine protease inhibitors. The encoded protein is produced by platelets and can bind to and inhibit the function of furin, a serine protease involved in platelet functions. In addition, this protein has been found to enhance the mechanical stability of cell-cell adhesion in the skin, and defects in this gene have been associated with an autosomal-recessive form of exfoliative ichthyosis. provided by RefSeq, Jan 2017
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"NM_001031848" in other vectors (4)
SKU Description Size Price
RC205135L3 Lenti ORF clone of Human serpin peptidase inhibitor, clade B (ovalbumin), member 8 (SERPINB8), transcript variant 3, Myc-DDK-tagged 10 ug
$600.00
RC205135L4 Lenti ORF clone of Human serpin peptidase inhibitor, clade B (ovalbumin), member 8 (SERPINB8), transcript variant 3, mGFP tagged 10 ug
$600.00
RG205135 SERPINB8 (tGFP-tagged) - Human serpin peptidase inhibitor, clade B (ovalbumin), member 8 (SERPINB8), transcript variant 3 10 ug
$489.00 $500.00
SC321661 SERPINB8 (untagged)-Human serpin peptidase inhibitor, clade B (ovalbumin), member 8 (SERPINB8), transcript variant 3 10 ug
$300.00

Plasmid Map for RC205135

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205135
Circular map for RC205135

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