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Cbl c (CBLC) (NM_012116) Human Tagged ORF Clone

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SKU
RC205130
CBLC (Myc-DDK-tagged)-Human Cas-Br-M (murine) ecotropic retroviral transforming sequence c (CBLC), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Cbl c
Synonyms CBL-3; CBL-SL; RNF57
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205130 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCTGGCGGTGGCCCCGTGGGGGCGACAGTGGGAAGAGGCCCGCGCCCTGGGCCGGGCAGTCAGGA
TGCTGCAGCGCCTAGAAGAGCAATGCGTCGACCCCCGGCTGTCCGTGAGTCCCCCTTCGCTGCGGGACCT
GCTGCCCCGCACAGCGCAGCTGCTTCGAGAGGTGGCCCATTCTCGGCGGGCGGCCGGCGGAGGCGGCCCC
GGGGGTCCCGGCGGCTCTGGGGACTTTCTACTCATCTACCTGGCCAATCTGGAGGCCAAGAGCAGGCAGG
TGGCCGCGCTGCTGCCTCCCCGGGGCCGAAGGAGTGCCAACGACGAGCTCTTCCGGGCGGGCTCCAGACT
CAGGCGACAGCTGGCCAAGCTGGCCATCATCTTCAGCCACATGCACGCAGAGCTGCACGCACTCTTCCCC
GGGGGAAAGTACTGTGGACACATGTACCAGCTCACCAAGGCCCCCGCCCACACCTTCTGGAGGGAAAGTT
GCGGAGCCCGGTGTGTGCTGCCCTGGGCTGAGTTTGAGTCCCTCCTGGGCACCTGCCACCCTGTGGAACC
AGGCTGCACAGCCCTGGCCTTGCGCACCACCATTGACCTCACCTGCAGCGGGCACGTGTCCATCTTCGAG
TTCGACGTCTTCACCAGGCTCTTTCAGCCATGGCCAACACTCCTCAAGAACTGGCAGCTCCTGGCAGTCA
ACCACCCAGGCTACATGGCCTTCCTCACCTATGATGAGGTCCAAGAGCGTCTGCAGGCCTGCAGGGACAA
GCCAGGCAGTTACATCTTCCGGCCCAGCTGTACTCGCCTGGGGCAGTGGGCCATCGGCTATGTGAGCTCA
GATGGCAGCATCCTGCAGACCATCCCTGCCAACAAACCCCTGTCCCAGGTGCTCCTGGAGGGACAGAAGG
ACGGCTTCTACCTCTACCCAGATGGAAAGACCCACAACCCAGACCTGACTGAGCTCGGCCAGGCAGAACC
CCAGCAGCGCATCCACGTGTCAGAGGAGCAGCTGCAGCTCTACTGGGCCATGGACTCCACATTTGAGCTC
TGCAAGATCTGTGCTGAGAGCAACAAGGATGTGAAGATTGAGCCGTGCGGGCACCTGCTCTGCAGCTGCT
GCCTGGCTGCCTGGCAGCACTCGGACAGCCAGACCTGCCCCTTCTGCCGCTGCGAGATCAAGGGCTGGGA
GGCCGTGAGTATCTACCAGTTCCACGGTCAGGCTACTGCTGAGGACCCAGGGAACAGCAGTGACCAGGAA
GGCAGGGAGTTGGAGCTGGGGCAGGTGCCCCTTTCGGCTCCTCCATTGCCCCCACGGCCAGATCTGCCCC
CCAGGAAGCCCAGAAATGCCCAGCCGAAAGTGAGACTCCTAAAGGGGAACTCCCCTCCAGCTGCGCTGGG
ACCCCAGGACCCTGCCCCGGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205130 protein sequence
Red=Cloning site Green=Tags(s)

MALAVAPWGRQWEEARALGRAVRMLQRLEEQCVDPRLSVSPPSLRDLLPRTAQLLREVAHSRRAAGGGGP
GGPGGSGDFLLIYLANLEAKSRQVAALLPPRGRRSANDELFRAGSRLRRQLAKLAIIFSHMHAELHALFP
GGKYCGHMYQLTKAPAHTFWRESCGARCVLPWAEFESLLGTCHPVEPGCTALALRTTIDLTCSGHVSIFE
FDVFTRLFQPWPTLLKNWQLLAVNHPGYMAFLTYDEVQERLQACRDKPGSYIFRPSCTRLGQWAIGYVSS
DGSILQTIPANKPLSQVLLEGQKDGFYLYPDGKTHNPDLTELGQAEPQQRIHVSEEQLQLYWAMDSTFEL
CKICAESNKDVKIEPCGHLLCSCCLAAWQHSDSQTCPFCRCEIKGWEAVSIYQFHGQATAEDPGNSSDQE
GRELELGQVPLSAPPLPPRPDLPPRKPRNAQPKVRLLKGNSPPAALGPQDPAPA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012116
ORF Size 1422 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012116.4
RefSeq Size 1591 bp
RefSeq ORF 1425 bp
Locus ID 23624
UniProt ID Q9ULV8
Cytogenetics 19q13.32
Protein Families Druggable Genome
Protein Pathways Chronic myeloid leukemia, Endocytosis, ErbB signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, Pathways in cancer, T cell receptor signaling pathway, Ubiquitin mediated proteolysis
MW 52.5 kDa
Summary This gene encodes a member of the Cbl family of E3 ubiquitin ligases. Cbl proteins play important roles in cell signaling through the ubiquitination and subsequent downregulation of tyrosine kinases. Expression of this gene may be restricted to epithelial cells, and alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Feb 2012
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Documents
Resources
"NM_012116" in other vectors (6)
SKU Description Size Price
RC205130L1 Lenti ORF clone of Human Cas-Br-M (murine) ecotropic retroviral transforming sequence c (CBLC), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC205130L2 Lenti ORF clone of Human Cas-Br-M (murine) ecotropic retroviral transforming sequence c (CBLC), transcript variant 1, mGFP tagged 10 ug
$757.00
RC205130L3 Lenti ORF clone of Human Cas-Br-M (murine) ecotropic retroviral transforming sequence c (CBLC), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC205130L4 Lenti ORF clone of Human Cas-Br-M (murine) ecotropic retroviral transforming sequence c (CBLC), transcript variant 1, mGFP tagged 10 ug
$757.00
RG205130 CBLC (tGFP-tagged) - Human Cas-Br-M (murine) ecotropic retroviral transforming sequence c (CBLC), transcript variant 1 10 ug
$489.00 $657.00
SC122781 CBLC (untagged)-Human Cas-Br-M (murine) ecotropic retroviral transforming sequence c (CBLC), transcript variant 1 10 ug
$457.00

Plasmid Map for RC205130

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205130
Circular map for RC205130

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