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PHPT1 (NM_014172) Human Tagged ORF Clone

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SKU
RC205124
PHPT1 (Myc-DDK-tagged)-Human phosphohistidine phosphatase 1 (PHPT1), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "PHPT1 or NM_014172"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PHPT1
Synonyms CGI-202; HEL-S-132P; HSPC141; PHP; PHP14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205124 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGTGGCGGACCTCGCTCTCATTCCTGATGTGGACATCGACTCCGACGGCGTCTTCAAGTATGTGC
TGATCCGAGTCCACTCGGCTCCCCGCTCCGGGGCTCCGGCTGCAGAGAGCAAGGAGATCGTGCGCGGCTA
CAAGTGGGCTGAGTACCATGCGGACATCTACGACAAAGTGTCGGGCGACATGCAGAAGCAAGGCTGCGAC
TGTGAGTGTCTGGGCGGCGGGCGCATCTCCCACCAGAGTCAGGACAAGAAGATTCACGTGTACGGCTATT
CCATGGCCTATGGTCCTGCCCAGCACGCCATTTCAACTGAGAAAATCAAAGCCAAGTACCCCGACTACGA
GGTCACCTGGGCTAACGACGGCTAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205124 protein sequence
Red=Cloning site Green=Tags(s)

MAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDMQKQGCD
CECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTWANDGY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014172
ORF Size 375 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014172.6
RefSeq Size 1199 bp
RefSeq ORF 378 bp
Locus ID 29085
UniProt ID Q9NRX4, V9HWC4
Cytogenetics 9q34.3
Domains Ocnus
Protein Families Druggable Genome
Protein Pathways Fructose and mannose metabolism, Metabolic pathways, Riboflavin metabolism, Thiamine metabolism
MW 13.8 kDa
Summary This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. provided by RefSeq, Dec 2013
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Documents
Resources
"NM_014172" in other vectors (6)
SKU Description Size Price
RC205124L1 Lenti ORF clone of Human phosphohistidine phosphatase 1 (PHPT1), transcript variant 3, Myc-DDK-tagged 10 ug
$450.00
RC205124L2 Lenti ORF clone of Human phosphohistidine phosphatase 1 (PHPT1), transcript variant 3, mGFP tagged 10 ug
$450.00
RC205124L3 Lenti ORF clone of Human phosphohistidine phosphatase 1 (PHPT1), transcript variant 3, Myc-DDK-tagged 10 ug
$450.00
RC205124L4 Lenti ORF clone of Human phosphohistidine phosphatase 1 (PHPT1), transcript variant 3, mGFP tagged 10 ug
$450.00
RG205124 PHPT1 (tGFP-tagged) - Human phosphohistidine phosphatase 1 (PHPT1), transcript variant 3 10 ug
$489.00
SC108149 PHPT1 (untagged)-Human phosphohistidine phosphatase 1 (PHPT1), transcript variant 3 10 ug
$150.00

Plasmid Map for RC205124

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205124
Circular map for RC205124

file-alt Citations

Histidine dephosphorylation of the Gβ protein GPB-1 promotes axon regeneration in C. elegans
Sakai, Hanafusa, Hisamoto et al
EMBO Rep (2022) 23 (12), e55076 DOI: 10.15252/embr.202255076
Cited by 8
AI Summary: In this study, the Myc-FLAG-PHPT1 plasmid (OriGene, RC205124) was used to investigate the role of protein histidine phosphatase in axon regeneration, enabling the authors to analyze the effects of PHPT1 overexpression on histidine dephosphorylation and neuronal repair mechanisms.
Snippet: …In this study, we show that PHIP‐1, a C. elegans ortholog of the mammalian PHPT1 protein, promotes axon regenerat;- Histidine phosphorylation is an emerging noncanonical protein phosphorylation in animals, yet its physiological role remains largely unexplored. …The Myc-FLAG-PHPT1 plasmid was purchased from OriGene (RC205124). …
Showing 1-1 of 1 papers.
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