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CHAC2 (NM_001008708) Human Tagged ORF Clone

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SKU
RC205083
CHAC2 (Myc-DDK-tagged)-Human ChaC, cation transport regulator homolog 2 (E. coli) (CHAC2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "CHAC2 or NM_001008708"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CHAC2
Synonyms GCG1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205083 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGGTTTTTGGTTACGGGTCCCTGATCTGGAAGGTGGATTTCCCCTATCAGGACAAGCTGGTCGGAT
ACATCACCAACTACAGCAGGCGCTTCTGGCAGGGCAGCACGGACCACCGCGGGGTCCCCGGCAAGCCTGG
AAGAGTTGTGACTCTTGTTGAAGATCCTGCGGGATGTGTATGGGGTGTTGCTTACAGATTGCCAGTAGGA
AAGGAAGAAGAAGTAAAAGCATACCTTGACTTCAGAGAAAAAGGAGGCTACAGAACCACAACAGTCATTT
TTTATCCAAAAGATCCCACAACAAAACCATTCAGTGTATTGCTATATATTGGAACATGTGATAATCCTGA
TTATCTTGGTCCTGCACCTCTGGAAGACATTGCTGAACAAATTTTTAATGCAGCTGGTCCAAGTGGAAGA
AATACAGAATATCTTTTTGAACTTGCAAATTCTATTAGGAACCTTGTGCCAGAAGAAGCAGATGAGCATC
TTTTCGCTTTGGAAAAATTAGTAAAGGAACGTTTAGAAGGGAAACAGAACCTCAATTGCATA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205083 protein sequence
Red=Cloning site Green=Tags(s)

MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKPGRVVTLVEDPAGCVWGVAYRLPVG
KEEEVKAYLDFREKGGYRTTTVIFYPKDPTTKPFSVLLYIGTCDNPDYLGPAPLEDIAEQIFNAAGPSGR
NTEYLFELANSIRNLVPEEADEHLFALEKLVKERLEGKQNLNCI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001008708
ORF Size 552 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001008708.4
RefSeq Size 1305 bp
RefSeq ORF 555 bp
Locus ID 494143
UniProt ID Q8WUX2
Cytogenetics 2p16.2
MW 20.9 kDa
Summary The protein encoded by this gene is a gamma-glutamyl cyclotransferase that catalyzes the conversion of glutathione to 5-oxoproline and cysteinylglycine. It is thought that this gene is upregulated in response to endoplasmic reticulum stress and that the glutathione depletion enhances apoptosis. provided by RefSeq, Sep 2016
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"NM_001008708" in other vectors (4)
SKU Description Size Price
RC205083L3 Lenti ORF clone of Human ChaC, cation transport regulator homolog 2 (E. coli) (CHAC2), Myc-DDK-tagged 10 ug
$600.00
RC205083L4 Lenti ORF clone of Human ChaC, cation transport regulator homolog 2 (E. coli) (CHAC2), mGFP tagged 10 ug
$600.00
RG205083 CHAC2 (tGFP-tagged) - Human ChaC, cation transport regulator homolog 2 (E. coli) (CHAC2) 10 ug
$500.00
SC301349 CHAC2 (untagged)-Human ChaC, cation transport regulator homolog 2 (E. coli) (CHAC2) 10 ug
$330.00

Plasmid Map for RC205083

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205083
Circular map for RC205083

file-alt Citations

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