SLC22A8 (NM_004254) Human Tagged ORF Clone
SKU
RC205056
SLC22A8 (Myc-DDK-tagged)-Human solute carrier family 22 (organic anion transporter), member 8 (SLC22A8), transcript variant 1
$757.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | SLC22A8 |
| Synonyms | OAT3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004254 |
| ORF Size | 1626 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004254.4 |
| RefSeq Size | 2207 bp |
| RefSeq ORF | 1629 bp |
| Locus ID | 9376 |
| UniProt ID | Q8TCC7 |
| Cytogenetics | 11q12.3 |
| Protein Families | Transmembrane |
| MW | 59.7 kDa |
| Summary | This gene encodes a protein involved in the sodium-independent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and appears to be localized to the basolateral membrane of the kidney. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. provided by RefSeq, May 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004254" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205056L1 | Lenti ORF clone of Human solute carrier family 22 (organic anion transporter), member 8 (SLC22A8), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,057.00
|
|
| RC205056L2 | Lenti ORF clone of Human solute carrier family 22 (organic anion transporter), member 8 (SLC22A8), transcript variant 1, mGFP tagged | 10 ug |
$1,057.00
|
|
| RC205056L3 | Lenti ORF clone of Human solute carrier family 22 (organic anion transporter), member 8 (SLC22A8), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,057.00
|
|
| RC205056L4 | Lenti ORF clone of Human solute carrier family 22 (organic anion transporter), member 8 (SLC22A8), transcript variant 1, mGFP tagged | 10 ug |
$1,057.00
|
|
| RG205056 | SLC22A8 (tGFP-tagged) - Human solute carrier family 22 (organic anion transporter), member 8 (SLC22A8), transcript variant 1 | 10 ug |
$957.00
|
|
| SC122673 | SLC22A8 (untagged)-Human solute carrier family 22 (organic anion transporter), member 8 (SLC22A8), transcript variant 1 | 10 ug |
$808.00
|
|
Citations
Estrone sulphate uptake by the microvillous membrane of placental syncytiotrophoblast is coupled to glutamate efflux
Lofthouse, Cleal, O'Kelly et al
Biochem. Biophys. Res. Commun. (2018)
Lofthouse, Cleal, O'Kelly et al
Biochem. Biophys. Res. Commun. (2018)
Identification of OAT1/OAT3 as Contributors to Cisplatin Toxicity
Hu, Leblanc, Gibson et al
Clin Transl Sci (2017)
Hu, Leblanc, Gibson et al
Clin Transl Sci (2017)
AI Summary: OriGene products, specifically full-length human OCT2, OAT1, OAT3, and mouse Oat1/Oat3 cDNA clones, were used to assess transporter function and elucidate the role of OAT1/OAT3 in cisplatin-induced nephrotoxicity in this study.
Snippet: Assessment of transporter function Full-length human OCT2, OAT1/OAT3, and mouse Oat1/Oat3 were obtained from Origene (Rockville, MD). Fusion-flag fragments were amplified by high-fidelity polymerase chain reaction (PCR). ... All 2 versions Cite SaveSaving
Interactions of 172 plant extracts with human organic anion transporter 1 (SLC22A6) and 3 (SLC22A8): a study on herb-drug interactions
Lu, Lu, Li et al
PeerJ (2017) 5, e3333 DOI: 10.7717/peerj.3333
Lu, Lu, Li et al
PeerJ (2017) 5, e3333 DOI: 10.7717/peerj.3333
AI Summary: OriGene contributed to this study by providing the cDNAs of human OAT1 (NM_004790) and OAT3 (NM_004254), which were essential for establishing HEK293 cell lines stably expressing these transporters to assess herb-drug interactions.
Snippet: In brief, the cDNAs of OAT1 (GenBank accession number NM_004790) and OAT3 (GenBank accession number NM_004254) were obtained from OriGene (Rockville, MD, USA), and sub-cloned into pcDNA5/FRT (Invitrogen). ... All 2 versions Cite SaveSaving
Stronger Uricosuric Effects of the Novel Selective URAT1 Inhibitor UR-1102 Lowered Plasma Urate in Tufted Capuchin Monkeys to a Greater Extent than Benzbromarone
Ahn, Ohtomo, Kiyokawa et al
J. Pharmacol. Exp. Ther. (2016) 357 (1), 157-66 DOI: 10.1124/jpet.115.231647
Ahn, Ohtomo, Kiyokawa et al
J. Pharmacol. Exp. Ther. (2016) 357 (1), 157-66 DOI: 10.1124/jpet.115.231647
Cited by 62
Sex-, Species-, and Tissue-Specific Metabolism of Empagliflozin in Male Mouse Kidney Forms an Unstable Hemiacetal Metabolite (M466/2) That Degrades to 4-Hydroxycrotonaldehyde, a Reactive and Cytotoxic Species
Taub, Ludwig-Schwellinger, Ishiguro et al
Chem. Res. Toxicol. (2015)
Taub, Ludwig-Schwellinger, Ishiguro et al
Chem. Res. Toxicol. (2015)
Cited by 34
AI Summary: OriGene contributed to this study by providing plasmid vectors (pCMV6-ENTRY) containing cDNA for various transporters—including oat1, oat3, oct1, oct2, oatp1a1, sglt1, and sglt2—which were used to investigate empagliflozin transport in transfected HEK293 cells.
Snippet: commercial suppliers. Plasmid vectors (pCMV6-ENTRY) containing cDNA for target transporters (oat1, oat3, oct1, oct2, oatp1a1, sglt1and sglt2) were obtained from Origene (Rockville, MD, USA). The plasmid vector, pSPORT1, containing either Oct1 or Oct2 were ... Cite SaveSaving
Showing 1-5 of 5 papers.
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