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BTBD14A (NACC2) (NM_144653) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC204959
NACC2 (Myc-DDK-tagged)-Human NACC family member 2, BEN and BTB (POZ) domain containing (NACC2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $546.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BTBD14A
Synonyms BEND9; BTBD14; BTBD14A; BTBD31; NAC-2; RBB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204959 representing NM_144653.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTCTCAGATGCTGCACATCGAGATCCCCAACTTCGGGAACACAGTGCTGGGCTGCCTGAACGAGCAG
CGCCTGCTGGGCCTCTACTGCGATGTGTCCATCGTGGTCAAGGGCCAGGCCTTCAAGGCCCACCGGGCG
GTGCTGGCCGCCAGCAGCCTCTACTTCCGCGACCTGTTCAGCGGCAACAGCAAGAGCGCCTTCGAGCTG
CCCGGCTCCGTGCCGCCCGCCTGCTTCCAGCAGATCCTGTCCTTCTGCTACACGGGCAGGCTCACCATG
ACGGCCAGCGAGCAGCTCGTGGTCATGTACACGGCCGGCTTCCTGCAGATCCAGCACATCGTGGAGCGC
GGCACCGACCTCATGTTCAAGGTGAGCTCGCCCCACTGCGACTCGCAGACCGCTGTGATCGAGGACGCC
GGCTCCGAGCCCCAGAGCCCCTGCAACCAGCTGCAGCCGGCCGCCGCCGCCGCGGCCCCCTACGTCGTG
TCCCCCTCGGTGCCCATCCCGCTGCTGACCCGAGTAAAGCATGAAGCCATGGAGCTGCCGCCGGCCGGC
CCAGGCCTGGACCCCAAGCGCCCGCTGGAGACGGGGCCCCGGGACGGCGTGGCGGTGGCCGCGGGGGCT
GCGGTGGCGGCGGGCACAGCCCCTCTCAAACTGCCCCGTGTCTCCTACTACGGGGTGCCCAGCCTGGCC
ACCCTCATCCCCGGCATCCAGCAGATGCCCTACCCCCAGGGGGAGCGGACCAGTCCAGGCGCCAGCAGC
CTGCCCACCACCGACAGCCCCACCTCGTACCACAACGAGGAGGACGAGGAGGACGACGAGGCCTACGAC
ACCATGGTGGAGGAGCAGTACGGCCAGATGTACATCAAGGCCTCCGGCAGCTATGCAGTGCAAGAGAAG
CCTGAGCCAGTGCCGCTGGAGAGCCGCTCCTGCGTCCTCATCCGCCGCGACCTCGTGGCCCTACCTGCC
AGCCTCATCAGCCAGATCGGATACCGCTGCCATCCCAAGCTCTACTCGGAAGGGGACCCCGGGGAGAAG
CTGGAGCTGGTGGCAGGCTCTGGGGTCTACATCACACGCGGCCAGCTGATGAACTGCCACCTGTGTGCA
GGGGTGAAGCATAAGGTCTTGCTGCGGAGGCTCCTGGCCACCTTCTTTGACAGGAACACGCTGGCCAAC
AGCTGCGGGACTGGCATCCGCTCGTCCACCAGCGACCCCAGCCGGAAGCCGCTGGACAGCCGGGTCCTG
AACGCTGTGAAATTGTACTGTCAGAACTTCGCCCCCAGCTTCAAGGAGAGCGAGATGAACGTGATCGCC
GCGGACATGTGCACCAACGCCCGCCGCGTTCGCAAGCGCTGGCTGCCCAAGATCAAGTCCATGCTGCCG
GAGGGCGTGGAGATGTACCGCACGGTCATGGGCTCCGCCGCCGCCAGCGTGCCCCTCGACCCCGAGTTC
CCGCCTGCCGCGGCACAGGTGTTCGAGCAACGCATCTACGCCGAGCGGCGGGGCGACGCCGCCACCATC
GTGGCTCTGAGAACTGACGCCGTGAATGTTGACCTGAGTGCCGCCGCCAACCCCGCCTTCGACGCCGGC
GAGGAGGTGGACGGGGCTGGCTCGGTCATCCAGGAGGTGGCCGCCCCCGAGCCGCTGCCCGCCGATGGC
CAGAGCCCCCCACAGCCCTTTGAGCAGGGCGGGGGCGGCCCCAGCAGGCCCCAGACGCCGGCGGCCGCG
GCCCGGAGGCCGGAGGGCACCTATGCAGGGACCTTG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Protein Sequence
>Peptide sequence encoded by RC204959
Blue=ORF Red=Cloning site Green=Tag(s)

MSQMLHIEIPNFGNTVLGCLNEQRLLGLYCDVSIVVKGQAFKAHRAVLAASSLYFRDLFSGNSKSAFEL
PGSVPPACFQQILSFCYTGRLTMTASEQLVVMYTAGFLQIQHIVERGTDLMFKVSSPHCDSQTAVIEDA
GSEPQSPCNQLQPAAAAAAPYVVSPSVPIPLLTRVKHEAMELPPAGPGLDPKRPLETGPRDGVAVAAGA
AVAAGTAPLKLPRVSYYGVPSLATLIPGIQQMPYPQGERTSPGASSLPTTDSPTSYHNEEDEEDDEAYD
TMVEEQYGQMYIKASGSYAVQEKPEPVPLESRSCVLIRRDLVALPASLISQIGYRCHPKLYSEGDPGEK
LELVAGSGVYITRGQLMNCHLCAGVKHKVLLRRLLATFFDRNTLANSCGTGIRSSTSDPSRKPLDSRVL
NAVKLYCQNFAPSFKESEMNVIAADMCTNARRVRKRWLPKIKSMLPEGVEMYRTVMGSAAASVPLDPEF
PPAAAQVFEQRIYAERRGDAATIVALRTDAVNVDLSAAANPAFDAGEEVDGAGSVIQEVAAPEPLPADG
QSPPQPFEQGGGGPSRPQTPAAAARRPEGTYAGTL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144653
ORF Size 1761 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq Size 6899 bp
RefSeq ORF 1764 bp
Locus ID 138151
UniProt ID Q96BF6
Cytogenetics 9q34.3
Domains BTB
MW 62.9 kDa
Summary Functions as a transcriptional repressor through its association with the NuRD complex. Recruits the NuRD complex to the promoter of MDM2, leading to the repression of MDM2 transcription and subsequent stability of p53/TP53.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_144653" in other vectors (4)
SKU Description Size Price
RC204959L3 Lenti-ORF clone of NACC2 (Myc-DDK-tagged)-Human NACC family member 2, BEN and BTB (POZ) domain containing (NACC2) 10 ug
$846.00
RC204959L4 Lenti-ORF clone of NACC2 (mGFP-tagged)-Human NACC family member 2, BEN and BTB (POZ) domain containing (NACC2) 10 ug
$846.00
RG204959 NACC2 (tGFP-tagged) - Human NACC family member 2, BEN and BTB (POZ) domain containing (NACC2) 10 ug
$746.00
SC319661 NACC2 (untagged)-Human NACC family member 2, BEN and BTB (POZ) domain containing (NACC2) 10 ug
$547.00

Plasmid Map for RC204959

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204959
Circular map for RC204959

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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