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TMC6 (NM_007267) Human Tagged ORF Clone

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SKU
RC204936
TMC6 (Myc-DDK-tagged)-Human transmembrane channel-like 6 (TMC6), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $737.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TMC6
Synonyms EV1; EVER1; EVIN1; LAK-4P; lnc; TNRC6C-AS1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204936 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCAGCCACTGGCCTTCATCCTCGATGTCCCTGAGACCCCAGGGGACCAGGGCCAGGGCCCCAGCC
CCTATGATGAAAGCGAAGTGCACGACTCCTTCCAGCAGCTCATCCAGGAGCAGAGCCAGTGCACGGCCCA
GGAGGGGCTGGAGCTGCAGCAGAGAGAGCGGGAGGTGACAGGAAGTAGCCAGCAGACACTCTGGCGGCCC
GAGGGCACCCAGAGCACGGCCACACTCCGCATCCTGGCCAGCATGCCCAGCCGCACCATTGGCCGCAGCC
GAGGTGCCATCATCTCCCAGTACTACAACCGCACGGTGCAGCTTCGGTGCAGGAGCAGCCGGCCCCTGCT
CGGGAACTTTGTCCGCTCCGCCTGGCCCAGCCTCCGCCTGTACGACCTGGAGCTGGACCCCACGGCCCTG
GAGGAGGAGGAGAAGCAGAGCCTCCTGGTGAAGGAGTTCCAGAGCCTGGCAGTGGCACAGCGGGACCACA
TGCTTCGCGGGATGCCCTTAAGCCTGGCTGAGAAACGCAGCCTGCGAGAGAAGAGCAGGACCCCGAGGGG
GAAGTGGAGGGGCCAGCCGGGCAGCGGCGGGGTCTGCTCCTGCTGTGGCCGGCTCAGATATGCCTGCGTG
CTGGCCTTGCACAGCCTGGGCCTGGCGCTGCTCTCCGCCCTGCAGGCCCTGATGCCGTGGCGCTACGCCC
TGAAGCGCATCGGGGGCCAGTTCGGCTCCAGCGTGCTCTCCTACTTCCTCTTTCTCAAGACCCTGCTGGC
TTTCAATGCCCTCCTGCTGCTGCTGCTGGTGGCCTTCATCATGGGCCCTCAGGTCGCCTTCCCACCCGCC
CTGCCGGGCCCTGCCCCCGTCTGCACAGGCCTGGAGCTCCTCACAGGCGCGGGTTGCTTCACCCACACCG
TCATGTACTACGGCCACTACAGTAACGCCACGCTGAACCAGCCGTGTGGCAGCCCCCTGGATGGCAGCCA
GTGCACACCCAGGGTGGGTGGCCTGCCCTACAACATGCCCCTGGCCTACCTCTCCACTGTGGGCGTGAGC
TTCTTTATCACCTGCATCACCCTGGTGTACAGCATGGCTCACTCTTTCGGGGAGAGCTACCGGGTGGGCA
GCACCTCTGGCATCCACGCCATCACCGTCTTCTGCTCCTGGGACTACAAGGTGACGCAGAAGCGGGCCTC
CCGCCTCCAGCAGGACAATATTCGCACCCGGCTGAAGGAGCTGCTGGCCGAGTGGCAGCTGCGGCACAGC
CCCAGGAGCGTGTGCGGGAGGCTGCGGCAGGCGGCTGTGCTGGGGCTTGTGTGGCTGCTGTGTCTGGGGA
CCGCGCTGGGCTGCGCCGTGGCCGTCCACGTCTTCTCGGAGTTCATGATCCAGAGTCCAGAGGCTGCTGG
CCAGGAGGCTGTGCTGCTGGTCCTGCCCCTGGTGGTTGGCCTCCTCAACCTGGGGGCCCCCTACCTGTGC
CGTGTCCTGGCCGCCCTGGAGCCGCATGACTCCCCGGTACTGGAGGTGTACGTGGCCATCTGCAGGAACC
TCATCCTCAAGCTGGCCATCCTGGGGACACTGTGCTACCACTGGCTGGGCCGCAGGGTGGGCGTCCTGCA
GGGCCAGTGCTGGGAGGATTTTGTGGGCCAGGAGCTGTACCGGTTCCTGGTGATGGACTTCGTCCTCATG
TTGCTGGACACGCTTTTTGGGGAACTGGTGTGGAGGATTATCTCCGAGAAGAAGCTGAAGAGGAGGCGGA
AGCCGGAGTTTGACATTGCCCGGAATGTCCTGGAGCTGATTTATGGGCAGACTCTGACCTGGCTGGGGGT
GCTCTTCTCGCCCCTCCTCCCCGCCGTGCAGATCATCAAGCTGCTGCTCGTCTTCTATGTCAAGAAGACC
AGCCTTCTGGCCAACTGCCAGGCGCCGCGCCGGCCCTGGCTGGCCTCACACATGAGCACCGTCTTCCTCA
CGCTGCTCTGCTTCCCCGCCTTCCTGGGCGCCGCTGTCTTCCTCTGCTACGCCGTCTGGCAGGTGAAGCC
CTCGAGCACCTGCGGCCCCTTCCGGACCCTGGACACCATGTACGAGGCCGGCAGGGTGTGGGTGCGCCAC
CTGGAGGCGGCAGGCCCCAGGGTCTCCTGGCTGCCCTGGGTGCACCGGTACCTGATGGAAAACACCTTCT
TTGTCTTCCTGGTGTCAGCCCTGCTGCTGGCCGTGATCTACCTCAACATCCAGGTGGTGCGGGGCCAGCG
CAAGGTCATCTGCCTGCTCAAGGAGCAGATCAGCAATGAGGGTGAGGACAAAATCTTCTTAATCAACAAG
CTTCACTCCATCTACGAGAGGAAGGAGAGGGAGGAGAGGAGCAGGGTTGGGACAACCGAGGAGGCTGCGG
CACCCCCTGCCCTGCTCACAGATGAACAGGATGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204936 protein sequence
Red=Cloning site Green=Tags(s)

MAQPLAFILDVPETPGDQGQGPSPYDESEVHDSFQQLIQEQSQCTAQEGLELQQREREVTGSSQQTLWRP
EGTQSTATLRILASMPSRTIGRSRGAIISQYYNRTVQLRCRSSRPLLGNFVRSAWPSLRLYDLELDPTAL
EEEEKQSLLVKEFQSLAVAQRDHMLRGMPLSLAEKRSLREKSRTPRGKWRGQPGSGGVCSCCGRLRYACV
LALHSLGLALLSALQALMPWRYALKRIGGQFGSSVLSYFLFLKTLLAFNALLLLLLVAFIMGPQVAFPPA
LPGPAPVCTGLELLTGAGCFTHTVMYYGHYSNATLNQPCGSPLDGSQCTPRVGGLPYNMPLAYLSTVGVS
FFITCITLVYSMAHSFGESYRVGSTSGIHAITVFCSWDYKVTQKRASRLQQDNIRTRLKELLAEWQLRHS
PRSVCGRLRQAAVLGLVWLLCLGTALGCAVAVHVFSEFMIQSPEAAGQEAVLLVLPLVVGLLNLGAPYLC
RVLAALEPHDSPVLEVYVAICRNLILKLAILGTLCYHWLGRRVGVLQGQCWEDFVGQELYRFLVMDFVLM
LLDTLFGELVWRIISEKKLKRRRKPEFDIARNVLELIYGQTLTWLGVLFSPLLPAVQIIKLLLVFYVKKT
SLLANCQAPRRPWLASHMSTVFLTLLCFPAFLGAAVFLCYAVWQVKPSSTCGPFRTLDTMYEAGRVWVRH
LEAAGPRVSWLPWVHRYLMENTFFVFLVSALLLAVIYLNIQVVRGQRKVICLLKEQISNEGEDKIFLINK
LHSIYERKEREERSRVGTTEEAAAPPALLTDEQDA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007267
ORF Size 2415 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007267.5, NP_009198.3
RefSeq Size 2789 bp
RefSeq ORF 2418 bp
Locus ID 11322
UniProt ID Q7Z403, A0A024R8V2
Cytogenetics 17q25.3
Protein Families Transmembrane
MW 90.1 kDa
Summary Epidermodysplasia verruciformis (EV) is an autosomal recessive dermatosis characterized by abnormal susceptibility to human papillomaviruses (HPVs) and a high rate of progression to squamous cell carcinoma on sun-exposed skin. EV is caused by mutations in either of two adjacent genes located on chromosome 17q25.3. Both of these genes encode integral membrane proteins that localize to the endoplasmic reticulum and are predicted to form transmembrane channels. This gene encodes a transmembrane channel-like protein with 10 transmembrane domains and 2 leucine zipper motifs. provided by RefSeq, Jul 2008
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"NM_007267" in other vectors (6)
SKU Description Size Price
RC204936L3 Lenti ORF clone of Human transmembrane channel-like 6 (TMC6), transcript variant 2, Myc-DDK-tagged 10 ug
$1,037.00
RC204936L4 Lenti ORF clone of Human transmembrane channel-like 6 (TMC6), transcript variant 2, mGFP tagged 10 ug
$1,037.00
RG204936 TMC6 (tGFP-tagged) - Human transmembrane channel-like 6 (TMC6), transcript variant 2 10 ug
$937.00
SC115626 TMC6 (untagged)-Human transmembrane channel-like 6 (TMC6), transcript variant 2 10 ug
$457.00
SC318289 TMC6 (untagged)-Human transmembrane channel-like 6 (TMC6), transcript variant 2 10 ug
$812.00
SC320504 TMC6 (untagged)-Human transmembrane channel-like 6 (TMC6), transcript variant 2 10 ug
$738.00

Plasmid Map for RC204936

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204936
Circular map for RC204936

file-alt Citations

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