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PSMD5 (NM_005047) Human Tagged ORF Clone

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SKU
RC204913
PSMD5 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 5 (PSMD5)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $469.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PSMD5
Synonyms S5B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204913 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCCCAGGCTTTGGCGCTGCTGAGAGAGGTAGCGAGGCTGGAAGCGCCGCTGGAGGAGCTACGCG
CGCTTCACTCCGTGCTGCAGGCAGTGCCGCTCAACGAGCTTCGCCAGCAAGCGGCGGAGCTGCGCCTCGG
CCCGCTCTTCTCCCTGCTTAACGAGAACCATAGGGAAAAGACTACTTTGTGTGTATCCATTCTGGAGAGA
TTGCTCCAAGCTATGGAACCGGTTCACGTGGCCCGGAACCTCAGGGTTGACCTGCAGAGGGGACTAATTC
ACCCTGATGATTCTGTAAAAATCCTCACTCTTTCCCAGATTGGAAGAATTGTTGAAAATTCTGATGCTGT
TACTGAGATTCTAAATAATGCTGAATTACTAAAACAAATTGTTTATTGCATTGGTGGAGAGAATCTATCT
GTAGCAAAAGCGGCTATCAAATCCCTGTCAAGAATATCACTAACCCAAGCTGGACTGGAGGCTTTATTTG
AAAGCAATCTGCTGGATGATTTGAAAAGTGTAATGAAAACAAATGACATTGTTCGATACAGGGTGTATGA
GCTAATTATAGAGATTTCTTCCGTGTCACCAGAATCTTTAAACTACTGTACCACAAGTGGATTGGTAACC
CAGCTCCTGAGAGAGCTGACTGGTGAGGATGTGTTGGTCAGAGCCACCTGTATAGAAATGGTGACATCAC
TGGCATATACTCATCATGGGCGACAATATCTTGCTCAAGAAGGAGTAATTGACCAAATTTCTAATATAAT
TGTTGGGGCAGATTCAGACCCTTTCTCTAGCTTCTATCTGCCAGGATTCGTGAAGTTTTTTGGAAACCTG
GCTGTCATGGATAGTCCTCAACAGATCTGTGAGCGTTATCCTATCTTTGTGGAAAAAGTCTTTGAAATGA
TAGAAAGTCAGGACCCCACTATGATTGGTGTAGCTGTAGACACAGTTGGAATCTTGGGATCCAATGTTGA
AGGAAAACAGGTTTTACAGAAAACAGGAACTCGCTTTGAACGCTTGCTTATGAGAATAGGACATCAATCA
AAGAATGCCCCAGTGGAGCTAAAAATTAGATGTTTGGATGCAATTTCATCTCTTCTGTACTTACCACCTG
AGCAGCAGACTGATGACCTTCTGAGGATGACAGAATCCTGGTTTTCTTCTTTATCTCGGGATCCACTGGA
GCTCTTCCGTGGCATTAGTAGTCAGCCCTTCCCTGAACTACACTGTGCTGCCTTAAAAGTGTTTACGGCC
ATTGCAAACCAACCCTGGGCTCAGAAACTTATGTTTAACAGTCCAGGTTTTGTAGAATATGTGGTGGACC
GGTCTGTGGAGCATGACAAAGCTTCAAAGGATGCCAAATATGAACTAGTGAAAGCACTTGCCAATTCCAA
GACAATTGCAGAAATCTTTGGGAACCCAAATTATTTGAGGCTCAGAACTTACCTGAGTGAAGGGCCATAC
TATGTGAAACCTGTTTCCACGACAGCAGTAGAAGGAGCCGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204913 protein sequence
Red=Cloning site Green=Tags(s)

MAAQALALLREVARLEAPLEELRALHSVLQAVPLNELRQQAAELRLGPLFSLLNENHREKTTLCVSILER
LLQAMEPVHVARNLRVDLQRGLIHPDDSVKILTLSQIGRIVENSDAVTEILNNAELLKQIVYCIGGENLS
VAKAAIKSLSRISLTQAGLEALFESNLLDDLKSVMKTNDIVRYRVYELIIEISSVSPESLNYCTTSGLVT
QLLRELTGEDVLVRATCIEMVTSLAYTHHGRQYLAQEGVIDQISNIIVGADSDPFSSFYLPGFVKFFGNL
AVMDSPQQICERYPIFVEKVFEMIESQDPTMIGVAVDTVGILGSNVEGKQVLQKTGTRFERLLMRIGHQS
KNAPVELKIRCLDAISSLLYLPPEQQTDDLLRMTESWFSSLSRDPLELFRGISSQPFPELHCAALKVFTA
IANQPWAQKLMFNSPGFVEYVVDRSVEHDKASKDAKYELVKALANSKTIAEIFGNPNYLRLRTYLSEGPY
YVKPVSTTAVEGAE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005047
ORF Size 1512 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005047.4
RefSeq Size 3504 bp
RefSeq ORF 1515 bp
Locus ID 5711
UniProt ID Q16401
Cytogenetics 9q33.2
MW 56.2 kDa
Summary The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a non-ATPase subunit of the 19S regulator base that functions as a chaperone protein during 26S proteasome assembly. provided by RefSeq, Jul 2012
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Documents
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"NM_005047" in other vectors (6)
SKU Description Size Price
RC204913L1 Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 5 (PSMD5), Myc-DDK-tagged 10 ug
$769.00
RC204913L2 Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 5 (PSMD5), mGFP tagged 10 ug
$769.00
RC204913L3 Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 5 (PSMD5), Myc-DDK-tagged 10 ug
$769.00
RC204913L4 Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 5 (PSMD5), mGFP tagged 10 ug
$769.00
RG204913 PSMD5 (tGFP-tagged) - Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 5 (PSMD5) 10 ug
$489.00 $669.00
SC321629 PSMD5 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 5 (PSMD5) 10 ug
$470.00

Plasmid Map for RC204913

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204913
Circular map for RC204913

file-alt Citations

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