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GKAP1 (NM_025211) Human Tagged ORF Clone

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SKU
RC204911
GKAP1 (Myc-DDK-tagged)-Human G kinase anchoring protein 1 (GKAP1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "GKAP1 or NM_025211"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GKAP1
Synonyms FKSG21; GKAP42
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204911 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCAGCAGTACTTAGTTCTGTTCCCACCACCGCTTCTCGTTTTGCCCTGTTACAAGTGGATAGTG
GCAGTGGCTCTGATTCTGAACCTGGAAAAGGTAAAGGTCGAAATACTGGAAAGTCTCAAACTTTAGGAAG
CAAGTCAACTACAAATGAGAAAAAAAGAGAGAAAAGAAGAAAAAAGAAGGAACAGCAACAGAGTGAAGCA
AATGAGCTCAGGAATCTTGCTTTTAAGAAAATTCCCCAGAAATCCTCCCATGCTGTTTGTAACGCTCAAC
ATGATCTTCCATTGTCAAACCCAGTACAGAAGGATTCACGAGAAGAAAATTGGCAAGAGTGGAGACAAAG
AGATGAGCAGCTGACATCTGAAATGTTTGAAGCAGATCTTGAGAAGGCATTGTTACTAAGTAAACTAGAA
TATGAAGAGCACAAAAAGGAGTATGAAGATGCTGAAAATACTTCAACTCAGTCCAAAGTTATGAATAAAA
AAGATAAAAGAAAGAATCATCAGGGAAAAGACAGACCTCTCACAGTATCACTAAAAGATTTTCATTCGGA
AGATCACATTAGTAAAAAGACTGAGGAATTGAGTTCTTCTCAGACTTTATCACATGATGGAGGATTCTTC
AATAGACTGGAAGATGATGTTCATAAAATTCTTATTAGAGAAAAACGAAGAGAACAGCTTACAGAATATA
ATGGAACAGATAATTGTACAGCTCATGAACACAACCAGGAAGTGGTTCTGAAAGATGGAAGAATTGAAAG
ACTAAAGTTAGAGCTTGAAAGGAAAGATGCTGAAATCCAGAAGCTGAAAAATGTAATCACTCAATGGGAG
GCAAAGTATAAGGAAGTAAAGGCAAGAAATGCACAATTATTGAAAATGCTTCAGGAAGGTGAAATGAAAG
ATAAGGCAGAAATACTTCTGCAAGTTGATGAATCACAAAGTATCAAGAATGAGCTCACTATTCAGGTGAC
TTCACTTCATGCTGCATTAGAACAAGAAAGATCTAAAGTGAAAGTATTACAAGCAGAGTTAGCCAAATAC
CAGGGTGGCAGAAAAGGGAAAAGAAACTCTGAATCCGACCAGTGTAGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204911 protein sequence
Red=Cloning site Green=Tags(s)

MASAVLSSVPTTASRFALLQVDSGSGSDSEPGKGKGRNTGKSQTLGSKSTTNEKKREKRRKKKEQQQSEA
NELRNLAFKKIPQKSSHAVCNAQHDLPLSNPVQKDSREENWQEWRQRDEQLTSEMFEADLEKALLLSKLE
YEEHKKEYEDAENTSTQSKVMNKKDKRKNHQGKDRPLTVSLKDFHSEDHISKKTEELSSSQTLSHDGGFF
NRLEDDVHKILIREKRREQLTEYNGTDNCTAHEHNQEVVLKDGRIERLKLELERKDAEIQKLKNVITQWE
AKYKEVKARNAQLLKMLQEGEMKDKAEILLQVDESQSIKNELTIQVTSLHAALEQERSKVKVLQAELAKY
QGGRKGKRNSESDQCR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025211
ORF Size 1098 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025211.4
RefSeq Size 1891 bp
RefSeq ORF 1101 bp
Locus ID 80318
UniProt ID Q5VSY0
Cytogenetics 9q21.32
Protein Families Druggable Genome
MW 42.1 kDa
Summary This gene encodes a protein that is highly similar to the mouse cGMP-dependent protein kinase anchoring protein 42kDa. The mouse protein has been found to localize with the Golgi and recruit cGMP-dependent protein kinase I alpha to the Golgi in mouse testes. It is thought to play a role in germ cell development. Transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Oct 2008
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"NM_025211" in other vectors (6)
SKU Description Size Price
RC204911L1 Lenti ORF clone of Human G kinase anchoring protein 1 (GKAP1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC204911L2 Lenti ORF clone of Human G kinase anchoring protein 1 (GKAP1), transcript variant 1, mGFP tagged 10 ug
$757.00
RC204911L3 Lenti ORF clone of Human G kinase anchoring protein 1 (GKAP1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC204911L4 Lenti ORF clone of Human G kinase anchoring protein 1 (GKAP1), transcript variant 1, mGFP tagged 10 ug
$757.00
RG204911 GKAP1 (tGFP-tagged) - Human G kinase anchoring protein 1 (GKAP1), transcript variant 1 10 ug
$489.00 $657.00
SC122982 GKAP1 (untagged)-Human G kinase anchoring protein 1 (GKAP1), transcript variant 1 10 ug
$457.00

Plasmid Map for RC204911

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204911
Circular map for RC204911

file-alt Citations

USP8 Deubiquitinates SHANK3 to Control Synapse Density and SHANK3 Activity-dependent Protein Levels
Kerrisk Campbell, Sheng
J. Neurosci. (2018) 38 (23), 5289-5301 DOI: 10.1523/JNEUROSCI.3305-17.2018
AI Summary: OriGene products, including Myc-tagged rat USP8 cDNA and rat USP8 shRNA constructs, were used in this study to manipulate USP8 expression and investigate its role in regulating SHANK3 protein levels and synapse density.
Snippet: Human SHANK2 Myc-tagged pCMV, human GKAP pCMV, and Myc-tagged rat 119 USP8 cDNAs were purchased (OriGene Technologies) Rat USP8 shRNA constructs were purchased 121 (OriGene Technologies) and targeted the following sequences: USP8_A 122
Showing 1-1 of 1 papers.
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