PHF22 (INTS12) (NM_020395) Human Tagged ORF Clone

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SKU
RC204838
INTS12 (Myc-DDK-tagged)-Human integrator complex subunit 12 (INTS12), transcript variant 1
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PHF22
Synonyms INT12; PHF22; SBBI22
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204838 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCTACTGTGAACTTGGAACTTGATCCCATTTTTTTGAAAGCACTAGGTTTCTTGCATTCAAAGA
GTAAAGATTCTGCTGAAAAGCTAAAAGCACTGCTTGATGAATCTTTGGCTCGGGGCATTGATTCCAGTTA
CCGTCCATCTCAAAAGGATGTGGAGCCACCCAAAATTTCAAGCACAAAAAACATTTCCATTAAGCAAGAG
CCCAAAATATCATCCAGTCTTCCTTCTGGTAATAATAATGGCAAGGTCCTCACAACTGAAAAGGTAAAGA
AGGAAGCTGAAAAGAGACCTGCTGATAAAATGAAATCAGACATCACTGAAGGAGTTGATATTCCAAAGAA
ACCTAGATTGGAGAAACCAGAAACACAGTCATCTCCCATTACTGTCCAAAGTAGCAAGGATTTACCTATG
GCTGACCTTTCCAGTTTTGAGGAGACCAGTGCTGATGATTTTGCCATGGAGATGGGATTGGCCTGCGTTG
TTTGTAGGCAAATGATGGTGGCATCTGGCAATCAATTAGTAGAATGTCAGGAGTGCCATAATCTCTACCA
CCGAGATTGTCATAAACCCCAGGTGACAGACAAGGAAGCGAATGACCCTCGCCTGGTGTGGTATTGTGCC
CGATGTACCAGACAAATGAAAAGAATGGCTCAAAAAACTCAGAAACCACCGCAGAAACCAGCCCCTGCAG
TTGTTTCTGTAACTCCAGCTGTCAAAGATCCATTGGTTAAGAAACCAGAAACTAAACTGAAACAAGAGAC
AACTTTTCTAGCGTTTAAGAGAACAGAAGTCAAGACATCCACAGTTATTTCAGGAAATTCTTCTAGTGCC
AGCGTTTCCTCGTCAGTAACTAGTGGCTTAACTGGATGGGCAGCTTTTGCAGCCAAAACTTCCTCTGCTG
GTCCTTCAACAGCAAAATTGAGTTCAACAACACAAAACAATACTGGGAAACCTGCTACTTCGTCAGCTAA
CCAGAAACCTGTGGGTTTGACTGGTCTGGCAACATCATCCAAAGGTGGAATAGGTTCCAAAATAGGTTCC
AATAACAGCACTACGCCCACTGTACCTTTAAAACCACCTCCACCTCTAACCTTGGGTAAAACTGGCCTTA
GTCGCTCAGTTAGTTGTGACAATGTCAGCAAAGTAGGTCTTCCTAGTCCAAGTAGTTTAGTTCCAGGAAG
CAGCAGCCAACTAAGTGGGAATGGAAATAGTGGAACATCAGGACCTAGTGGAAGTACTACCAGCAAAACT
ACTTCAGAATCCAGCAGCTCTCCCTCAGCATCCCTTAAAGGCCCAACTTCACAAGAATCACAGCTCAATG
CTATGAAGCGATTACAGATGGTCAAGAAGAAAGCTGCCCAAAAGAAACTCAAGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC204838 protein sequence
Red=Cloning site Green=Tags(s)

MAATVNLELDPIFLKALGFLHSKSKDSAEKLKALLDESLARGIDSSYRPSQKDVEPPKISSTKNISIKQE
PKISSSLPSGNNNGKVLTTEKVKKEAEKRPADKMKSDITEGVDIPKKPRLEKPETQSSPITVQSSKDLPM
ADLSSFEETSADDFAMEMGLACVVCRQMMVASGNQLVECQECHNLYHRDCHKPQVTDKEANDPRLVWYCA
RCTRQMKRMAQKTQKPPQKPAPAVVSVTPAVKDPLVKKPETKLKQETTFLAFKRTEVKTSTVISGNSSSA
SVSSSVTSGLTGWAAFAAKTSSAGPSTAKLSSTTQNNTGKPATSSANQKPVGLTGLATSSKGGIGSKIGS
NNSTTPTVPLKPPPPLTLGKTGLSRSVSCDNVSKVGLPSPSSLVPGSSSQLSGNGNSGTSGPSGSTTSKT
TSESSSSPSASLKGPTSQESQLNAMKRLQMVKKKAAQKKLKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020395
ORF Size 1386 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020395.4
RefSeq Size 1768 bp
RefSeq ORF 1389 bp
Locus ID 57117
UniProt ID Q96CB8, A0A024RDK4
Cytogenetics 4q24
Domains PHD
Protein Families Druggable Genome, Transcription Factors
MW 48.8 kDa
Summary INTS12 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 PubMed 16239144).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_020395" in other vectors (4)
SKU Description Size Price
RC204838L3 Lenti ORF clone of Human integrator complex subunit 12 (INTS12), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC204838L4 Lenti ORF clone of Human integrator complex subunit 12 (INTS12), transcript variant 1, mGFP tagged 10 ug
$757.00
RG204838 INTS12 (tGFP-tagged) - Human integrator complex subunit 12 (INTS12), transcript variant 1 10 ug
$489.00 $657.00
SC319540 INTS12 (untagged)-Human integrator complex subunit 12 (INTS12), transcript variant 1 10 ug
$457.00

Plasmid Map for RC204838

Download sequence for

Cloning scheme for RC204838

Circular map for RC204838

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